KOBALT KULLANIMININ STZ-DENEYSEL DİYABETİK SIÇANLARIN BÖBREK DOKULARINDA CAT, GSH-PX, SOD AKTİVİTELERİ VE LİPİD PEROKSİDASYONU DÜZEYLERİNE ETKİSİ

Serbest radikallerin diyabette çeşitli komplikasyonlara neden olduğu bildirilmektedir. Günümüze kadar yapılmış olan pekçok çalışma ile, serbest radikallerin oluşumunda geçiş metallerinin önemli rol oynadıkları gösterilmiştir. Öte yandan kobaltın kan şekerini düşürücü etkisi olduğu da öne sürülmektedir. Bu noktadan hareketle çalışmamızda streptozotozin (STZ) ile deneysel diyabet oluşturulan sıçanlara, kobalt (II) klorür uygulanmış (0,5 mM) ve 2, 4 ve 6. haftalarda böbrek doku örnekleri alınarak bu örneklerde katalaz, glutatyon peroksidaz, süperoksit dismutaz aktiviteleri ile TBARS düzeyleri ölçülmüştür. Diyabetik sıçanların böbrek dokularında, 2, 4 ve 6. haftalarda katalaz, glutatyon peroksidaz ve süperoksit dismutaz. aktivitelerinde kobalt uygulanışı ile birlikte azalma görülmektedir. Bunun yanında kobalt, diyabette artan TBARS düzeylerinde de düşüşe neden olmaktadır. Sonuç olarak, kobalt kullanımının diyabette böbrek dokularında, artan oksidan stresin azalmasında ve/veya önlenmesinde önemli rol oynayabileceği düşünülmektedir.

THE EFFECT OF COBALT SUPPLEMENTATION ON CAT, GSH-PX, SOD ACTIVITIES AND LIPID PEROXIDE LEVELS IN STZ-INDUCED EXPERIMENTAL DIABETIC RAT KIDNEY

It has been stated that free radicals cause several complications in diabetics. Several researches until now showed that, trace elements play an important role in formation of free radicals. On the other hand, it is claimed that cobalt has a blood glucose lowering effect. From this point of view, in streptozotocin (STZ) induced diabetic rats were given cobalt (II) chloride (0,5 mM), kidney samples were taken from each group at the 2nd, 4th and 6th weeks and the activities of catalase, glutathione peroxidase, superoxide dismu.tase and levels of TBARS were analysed. In STZ-induced diabetic rat kidney, it has been indicated that there was a decrease in the catalase, glutathione peroxidase and superoxide dismutase activities of the tissues upon administration. Moreover, cobalt also reduced TBARS levels which are increased in diabetes. As a result, we believe that at the early stage of diabetes, the cobalt dose which was used in this study can be considered as appropriate as regards regulation of oxidative and antioxidative systems.

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