[99mTc]Tc-NAB-PAKLİTAKSEL'İN İNSAN KOLOREKTAL ADENOKARSİNOMA HÜCRE HATTI ÜZERİNDE HÜCRE BAĞLANMA ÇALIŞMASI

Amaç: Spesifik olarak kanseri görüntüleyebilen yeni radyofarmasötiklerin geliştirilmesi önemli bir araştırma alanıdır. Bu nedenle bu çalışmanın amacı, yeni geliştirilen nanoradyofarmasötiğin ([99mTc]Tc-nab-PTX) HT-29 hücre hattına (insan kolorektal adenokarsinom hücreleri) hücre bağlanmasını araştırmaktır. Materyal ve Metot: Bu çalışmada, nab-PTX [99mTc]Tc ile işaretlenmiş ve işaretleme etkinliği ve in vitro stabilitesi radyoaktif ince tabaka kromatografisi (RTLC) ile araştırılmıştır. Daha sonra, [99mTc]Tc-nab-PTX'in hücre bağlanması, HT-29 hücre hattı kullanılarak gerçekleştirilmiştir. Sonuç ve Tartışma: Elde edilen sonuçlara göre, nab-PTX yüksek radyokimyasal saflıkla (>%99) radyoişaretlenmiştir. [99mTc]Tc-nab-PTX'in oda sıcaklığında 6 saate kadar ve hücre ortamında 2 saate kadar stabil olduğu bulunmuştur. [99mTc]Tc-nab-PTX'in HT-29 hücreleri üzerinde 30. dakikada R/H-[99mTc]NaTcO4'ten daha yüksek bir hücre bağlama aktivitesine sahip olduğu belirlenmiştir. Sonuçların [99mTc]Tc-nab-PTX ile gelecek in vivo çalışmalar için umut verici olduğu bulunmuştur.

Anahtar Kelimeler:

HT29 hücre hattı, nab-paklitaksel, radyoişaretleme, teknesyum-99m

CELL INCORPORATION STUDY OF [99mTc]Tc-NAB-PACLITAXEL ON HUMAN COLORECTAL ADENOCARCINOMA CELL LINE

Objective: The development of new radiopharmaceuticals capable of specifically imaging cancer is an important research area. For this reason, the aim of this study is to investigate cell binding of novel developed nanoradiopharmaceutical ([99mTc]Tc-nab-PTX) on HT-29 cell line (human colorectal adenocarcinoma cells). Material and Method: In this study, nab-PTX was labeled with [99mTc]Tc, and labeling efficiency and in vitro stability were investigated by radioactive thin layer chromatography (RTLC). Then, cell incorporation of [99mTc]Tc-nab-PTX was performed using HT-29 cell line. Result and Discussion: According to obtained results, nab-PTX was labeled with high radiochemical purity (>99%). The [99mTc]Tc-nab-PTX was found to be stable in saline for 6-h, and in cell medium up to 2-h. It was determined that [99mTc]Tc-nab-PTX had a greater cell binding activity on HT-29 cells than R/H-[99mTc]NaTcO4 at 30 min. The results were found to be promising for future in vivo studies with [99mTc]Tc-nab-PTX.

Keywords:

HT29 cell line, nab-paclitaxel, radiolabeling, technetium-99m,

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