İlyas DELİGÖZ, Abdullah BALTACI, Nejla ÇELİK, Sabriye ÖZDEMİR, Nesrin UZUNOĞULLARI, Nazlı KUTLUK YILMAZ

Türkiye’de Biberde Enfeksiyon Oluşturan Virüslerin Belirlenmesi

Biber (Capsicum annuum L.)’de çok sayıda virüs enfeksiyona neden olmakta ve önemli ürün kayıplarına yol açmaktadır. Bu çalışma kapsamında, 2018 yılında Türkiye biber üretiminin büyük bir bölümünün gerçekleştirildiği Antalya, Manisa, Çanakkale, Samsun ve Bursa illerinde biber yetiştirilen alanlardan virüs-benzeri simptom sergileyen 616 adet biber örneği toplanmıştır. Bu örnekler daha sonra Alfalfa mosaic virus (AMV), Cucumber mosaic virus (CMV), Impatiens necrotic spot virus (INSV), Pepper mild mottle virus (PMMoV), Potato virus Y (PVY), Tobacco etch virus (TEV), Tobacco mosaic virus (TMV), Tomato chlorotic spot virus (TCSV), Tomato mosaic virus (ToMV) ve Tomato spotted wilt virus (TSWV) poliklonal antiserumları kullanılarak Double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA) yöntemi ile test edilmiştir. Test sonuçlarına göre örneklerin % 55’inin TSWV, % 22.2’sinin CMV, %0.5’inin PMMoV, %0.5’inin PVY ve %0.3’ünün ise AMV ile tekli enfekteli olduğu belirlenmiştir. İncelenen örneklerin % 6.5’inin ise birden fazla virüs ile karışık enfekteli olduğu tespit edilmiştir. Karışık enfeksiyonlu örnekler içerisinde en yaygın olarak TSWV+CMV ikili enfeksiyonuna (% 4.5) rastlanılırken, bunu sırası ile; TSWV+PVY (% 0.6), TSWV+PMMoV (% 0.5), PMMoV+CMV (% 0.3) izlemiştir. Ayrıca, test edilen örneklerde TSWV+CMV+PVY (% 0.3) ve TSWV+CMV+PMMoV (% 0.2) üçlü karışık enfeksiyonu belirlenmiştir. Bununla birlikte, çalışmada test edilen biber örneklerinin hiçbirisinde ToMV, TMV, TEV, TCSV ve INSV tespit edilememiştir. Bu çalışmada tespit edilen PMMoV, Ege ve Marmara Bölgesi için ilk kayıt niteliğindedir.

Anahtar Kelimeler:

Capsicum annuum L, DAS-ELISA, Sürvey, Bulunma oranı, Capsicum annuum L., Survey, Incidence

Determination of Viruses Infecting Pepper in Turkey

Various viruses cause infection in pepper (Capsicum annuum L.) and can lead to significant crop losses. In this study, 616 pepper samples exhibiting virus-like symptoms were collected in 2018 from pepper production areas in Antalya, Manisa, Çanakkale Samsun and Bursa province, where most of Turkey's pepper production is carried out. Samples were tested using polyclonal antisera of Alfalfa mosaic virus (AMV), Cucumber mosaic virus (CMV), Impatiens necrotic spot virus (INSV), Pepper mild mottle virus (PMMoV), Potato virus Y (PVY), Tobacco etch virus (TEV), Tobacco mosaic virus (TMV), Tomato chlorotic spot virus (TSCV), Tomato mosaic virus (ToMV) and Tomato spotted wilt virus (TSWV) by Double antibody sandwich-enzyme linked immunosorbent assay DAS-ELISA. Based on the serological test results, TSWV, CMV, PMMoV, PVY and AMV were detected in 55, 22.2, 0.5, 0.5 and 0.3 % of the total tested samples, respectively. Mixed infections involving different combination of viruses were found in 6.5 % of samples. TSWV+CMV dual infection was the most common (4.5 %) among mixed-infected samples, followed by TSWV+PVY (0.6 %), TSWV+PMMoV (0.5 %), PMMoV+CMV (0.3 %). Also, TSWV+CMV+PVY (0.3 %) and TSWV+CMV+PMMoV (0.2 %) triple infections were determined in the tested samples. However, ToMV, TMV, TEV, TCSV and INSV were not detected in any of the pepper samples tested in this study. To our knowledge, this is the first report of PMMoV on pepper in the Aegean and Marmara Regions of Turkey.

Keywords:

Capsicum annuum L., Survey, Incidence, DAS-ELISA, ,

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