Hülya Akdemir, Tuğçe Akbulak, Veysel Süzerer, Doğa Kayıhan, İbrahim Koç, Yelda Özden Çiftçi

Farklı sitokinin ve şeker türlerinin alev ağacı bitkisinin in vitro çoğaltımına olan etkileri

Dünyada süs bitkilerine olan talep gün geçtikçe artmaktadır. Ancak, ticari bir süs bitkisi olan alev ağacının vejetatif yollar ile çoğaltılmasında kullanılan çeliklerde köklenme sorunlarına rastlanılmaktadır. Vejetatif üretimde karşılaşılan bu zorlukların aşılmasında birçok türde bitki doku kültürü tekniklerinden yararlanılmaktadır. Bu nedenle bu çalışmanın amacı ekonomik değere sahip olan alev ağacı bitkisinin mikroçoğaltımı için uygun bitki büyüme düzenleyicisi içeriğinin yarı-katı kültür sisteminde belirlenmesidir. Çalışmada farklı sitokinin tipleri [BA (6-benziladenin), KIN (Kinetin); TDZ (Tidiazuron); 2-iP (2 –izopentiladenin)] ve miktarları (0, 1, 2, 4 mg l-1) ile birlikte çeşitli karbon kaynakları (sukroz ve glikoz) ve miktarları (0, 15, 30 g l-1) yarı-katı besi ortamında denenmiştir. Alev ağacına ait gövde uçlarından en yüksek çoğaltım (%100) ve eksplant başına en fazla gövde oluşumu (3.7), 2 mg l-1 BA ve 30 g l-1 sukroz ile desteklenen yarı-katı MS besi ortamında elde edilmiştir. Oluşan gövdelerde en yüksek köklenme (%40) 4 mg l-1 IBA ile desteklenen yarı-katı besi ortamında 1 hafta kültürleme ve sonrasında oksin içermeyen besi ortamına aktarılma ile sağlanmıştır. Köklenen gövdeler daha sonra in vivo koşullara başarıyla iklimlendirilmişlerdir.

Influences of different types of cytokinin and sugar on in vitro proliferation of fraser photinia

There is an increasing demand to ornamental plants in the World. However, problems faced with the rooting of cuttings in the propagation of ornamental fraser photinia plants. Plant tissue culture techniques are used in several plant species to overcome such difficulties of vegetative production. Therefore, the aim of this study concerns the identification of optimum plant growth regulator content in semi-solid culture for the micropropagation of economically important fraser photinia. Thus, different cytokinin types [BA (6-benzyladenine), KIN (Kinetin); TDZ (Thidiazuron); 2-iP (2 –isopentyl adenine)] and amounts (0, 1, 2, 4 mg l-1) together with various carbon sources (sucrose and glycose) and amounts (0, 15, 30 g l-1) were assessed in semi-solid medium. The highest proliferation rate (100%) and the maximum number of shoots proliferated per explant (3.7) were obtained in shoot tips of fraser photinia on semi-solid MS medium supplemented with 2 mg l-1 BA and 30 g l-1 sucrose. The highest rooting (40%) was achieved in proliferated shoots on semi-solid medium supplemented with 4 mg l-1 IBA for 1 week followed by transfer of the microshoots to auxin-free medium. Rooted shoots were then successfully acclimatized to in vivo conditions.

Keywords:

6-benzyladenine, 2-isopentyladenin; kinetin; Photinia fraseri; thidiazuron,

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