Farklı büyüme düzenleyicilerin Türk kır çeltiği rejenerasyonuna etkisi

Çeltik bitkisi, tüm Dünya’da ve özellikle Asya kıtasında yetişen temel bir üründür. Çeltik, kuraklık stresi gibi farklı abiyotik ve biyotik stres faktörleri ile karşı karşıyadır. Türk kır çeltiği, kuraklık stres koşullarında yetişen ve büyük bir potansiyele sahip olan üstün çeltik türlerinden biridir. Bu çalışmanın amacı, BAP + NAA ve KIN + 2.4-D kombinasyonunun farklı konsantrasyonlardaki in vitro koşullarda yetiştirilen çeltik üzerindeki etkilerini incelemektir. Bu amaçla, başlangıç materyali olarak olgun çeltik tohumları kullanılmış olup direkt ve indirekt rejenerasyon sonuçları kaydedilmiştir. Gövde rejenerasyonu indüksiyonu için en iyi ortam, 1.5 mg L-1 BAP ve 0.5 NAA mg L-1 ile desteklenmiş MS1 olmuştur. Sonuç olarak, gövde nodların 21 gün içinde 2.4-D (2.0 mg L-1) içeren MS ortamı üzerinde maksimum kallus oluşumunun (% 65.54) elde edildiğini kaydedilmiştir. Bu çalışma ile Türk kır çeltiği için doku kültürü optimizasyonu geliştirirmiştir ve çeltik biyoteknolojisi açısından pozitif metotlar sunulmuştur.

Effects of different growth regulators on regeneration of Turkish upland rice

Rice is a staple crop grown in the world especially in the Asian region. The rice faces with different biotics and abiotic stress factors, especially drought stress. Turkish upland rice is one of the special rice types grown on drought stress condition, having a large potential value. Our aim was to analyse the effects of different concentrations of BAP+NAA and KIN+2,4-D combination on rice grown in vitro condition. For this purpose, mature seeds of Turkish upland rice were selected as starting material for direct/indirect regeneration response in serial treatment. The best medium for induction of shoot regeneration was MS1 supplemented with 1.5 mg L-1 BAP and 0.5 NAA mg L-1. The results showed that the maximum callus formation (65.54 %) was obtained on MS medium containing 2.4-D (2.0 mg L-1) within 21 days from nodes. With this study, we have developed a plant tissue culture for Turkish upland rice, suggesting positive methods for rice biotechnology. Rice is a staple crop grown in the world especially in the Asian region. The rice faces with different biotics and abiotic stress factors, especially drought stress. Turkish upland rice is one of the special rice types grown on drought stress condition, having a large potential value. Our aim was to analyse the effects of different concentrations of BAP+NAA and KIN+2,4-D combination on rice grown in vitro condition. For this purpose, mature seeds of Turkish upland rice were selected as starting material for direct/indirect regeneration response in serial treatment. The best medium for induction of shoot regeneration was MS1 supplemented with 1.5 mg L-1 BAP and 0.5 NAA mg L-1. The results showed that the maximum callus formation (65.54 %) was obtained on MS medium containing 2.4-D (2.0 mg L-1) within 21 days from nodes. With this study, we have developed a plant tissue culture for Turkish upland rice, suggesting positive methods for rice biotechnology.

Keywords:

Kallus, Bitki rejenerasyonu, BAP, NAA KIN, 2.4-D,

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