Yüksek Yağlı Diyet Ve Fibronektin Tip III Domein İçeren Protein 5 mRNA Ekspresyonu Arasındaki İlişki

Amaç: Hem kahverengi yağ dokusunda hemde kas dokusunda fibronektin tip III domein içeren protein 5 (FNDC5) bol bulunur. FNDC5 ve FNDC5’in fragmenti olan irisinin çeşitli etkilerinden dolayı obezite ile ilişkili metabolik hastalıkların tedavisinde tedavi edici molekül olarak kullanılabileceği ifade edilmektedir. Membran proteini olan FNDC5 ve irisin yakın zamanda bulunduğu için literatürde sınırlı sayıda çalışma bulunmaktadır. Bu çalışmanın amacı kas ve kahverengi yağ dokusunda yüksek yağlı diyet ve metabolik hastalıklarla ilişkili FNDC5 mRNA ekspresyonu arasındaki ilişkiyi incelemektir.Gereç ve Yöntemler: Çalışmada 18 adet 1,5-2 aylık erkek Sprague-Dawley sıçanlar rastgele her grupta 6 tane olacak şekilde 3 gruba ayrıldı. Sıçanlar gruplarına özgü yemle 85 gün beslendi. Beslenme süresinin bitiminde kas ve kahverengi yağ dokuları toplandı ve bu dokularda FNDC5 mRNA ekspresyon ölçümleri yapıldı.Bulgular: Kontrol grubu ile obez grubun FNDC5 mRNA seviyesi karşılaştırıldığında, kahverengi yağ dokusunda obez grupta kontrol grubuna göre daha yüksekti (p=0.016). Obez grup ile antioksidan grup karşılaştırıldığında ise, aynı dokuda obez grubun FNDC5 mRNA seviyesinin antioksidan gruptan daha yüksek olduğu tesbit edildi (p=0.010). Kas dokusuna gelince, bu dokuda antioksidan grupta kontrol grubuna göre daha düşük FNDC5 mRNA ekspresyonu ölçüldü (p=0.006). Tartışma ve Sonuç: Çalışmamız yüksek yağlı diyetin kahverengi yağ dokusundaki FNDC5 mRNA ekspresyonunu değiştirdiğini fakat kas dokusunda değiştirmediğini, bu nedenle yüksek yağlı diyetin kahverengi yağ dokusunda FNDC5 mRNA ekspresyonunun düzenlenmesinde etkili olabileceği anlaşılmaktadır.

Anahtar Kelimeler:

Kahverengi yağ dokusu, FNDC 5, yüksek yağlı diyet, obezite

The Relationship Between High-Fat Diet and Fibronectın Type III Domaın Containing Protein 5 mRNA Expression

Aim: Fibronectin type III domain containing protein 5 (FNDC5) is located in both muscle and brown adipose tissue. It is expressed that FNDC5 and irisin (fragment of FNDC5) may be use as a therapeutic molecule due to their effects for metabolic disease (related to obesity). Since irisin/FNDC5, a membrane protein, was recently revealed, there are limited number of studies about them. The purpose of this study was to assess the relationship between FNDC5 assosiated with metabolic disease and high-fat diet in muscle and brown adipose tissue.Materials and Methods: In the study, 18 male Sprague-Dawley rats, 1.5-2 months old were used. Rats were randomly chosen, and divided into 3 groups consisting of 6 rats. Rats were fed 85 days with low-fat standard diet or high-fat diet. In both the brown adipose tissue and muscle tissue, FNDC5 mRNA expressions were measured. Results: When the FNDC5 mRNA levels of control and obese groups were compared, it was determined that the FNDC5 mRNA levels of obese group was higher than that of the control group in the brown adipose tissue (p=0.016). When the FNDC5 mRNA levels of antioxidant and obese groups were examined, obese group’s levels was higher than that of the antioksidant group in the brown adipose tissue (p=0.010). As for the muscle tissue, FNDC5 mRNA levels were determined to be lower in the antioxidant group than in the control group (p=0.006). Discussion and Conclusion: Our study indicated that FNDC5 mRNA expression changed in the brown adipose tissue due to high-fat diet, but doesn’t change in the muscle tissue. Therefore, it is understood that high-fat diet may be effective in the regulation of FNDC5 mRNA expression in BAT.

Keywords:

Brown adipose tissue, FNDC 5, high-fat diet, obesity,

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