Reassessment of Mentha Species from Kunhar River Catchment Using Morphological and Molecular Markers

Mentha specimens collected from Kunhar River catchment of Hazara region were analyzed through numerical and molecular markers. For numerical analysis twenty two traits were used. Dendrogram analysis of morphological traits assorted the 25 Mentha collections into 4 groups viz, Group-A, B, C and D. Group-A showed 98% similarity (M. longifolia). Group-B showed 98% similarity (M. spicata). Group-C showed 92% similarity (M. arvensis) and Group-D showed 89% similarity (M. royleana). Molecular analyses were carried out through 11 RAPD primers. Primers amplification revealed high level of genetic diversity (0-100%) existed among the Mentha genotypes. Dendrogram analyses based upon the genetic distance estimates conferred the cluster analysis of the morphological traits. Furthermore, the variations among populations of M. royleana need further elaboration through additional marker assisted discrimination for establishing their taxonomic status.

Kaynakça

Anonim. 2017. Bizim Bitkiler, Türkiye bitkileri listesi. Damarlı Bitkiler. http://www.bizimbitkiler.org.tr.

Xi-ven, L., and I. C. Hedge. 1994. Flora of China. Science Press Beijing 17: 237-239.

Weishing, K. H. Nybom, K. Wolff, and W. Meyer. 1995. DNA isolation and purification. pp. 44-59. In: DNA fingerprinting in plants and fungi. CRC Press Boca Raton, Florida.

Sultana, K., and R. A. Qureshi. 2007. Check list of Basidiomycetes (Aphyllo. and Phragmo.) of Kaghan Valley-11. Pak. J. Bot. 39: 2629-2649.

Stewart, R. R. 1972. An Annotated Catalogue of Vascular Plants of West Pakistan and Kashmir. Fakhri Printing Press, Karachi.

Singh, T. P. A., and K. Sharma. 1986. Mentha taxonomic status as interpreted through cytology, genetics and phytochemistry. Indian Journal of Genetics 46: 198- 208.

Sharma, A. D., G. P. Kaur, and S. Prabhjeet. 2002. DNA isolation from dry and fresh samples of polysacchariderich plants. Plant Molecular Biology Reporter 20: 415- 415.

Pichersky, E. J., P. Noel, and N. Dudareva. 2006. Biosynthesis of plant. Plant Cell 7: 1085-1097.

Nasir, E. and S. I. Ali. 1975. Flora of Pak. No. 87. Department of Botany, Karachi University, Karachi. Pakistan.

Masotti, V., F. Juteau, J. M. Bessie`re, and J. Viano. 2003. Seasonal and Phenological Variations of the Essential Oil from the Narrow Endemic Species Artemisia molinieri and Its Biological Activities. J. Agric. Food Chem. 51: 7115-7121.

Malinvaud, E. 1880. Simple aperçu des hybridesdans le genre Mentha. Bulletin de la Société Botanique de France 27: 332-347.

Lodhi, A. M., Ye. Guang-Ning, F. W. Norman, and I. R. Bruce. 1994. A simple and efficient method for DNA extraction from grapevine cultivars, Vitis species and Ampelopsis. Plant Molecular Biology Reporter 1: 6-13.

Lawrence, B. M. 1978. A study of the monoterpene interrelationships in the genus Mentha with special reference to the origin of pulegone and menthofuran. Ph.D. Dissertation. Groningen University, Groningen, Netherlands.

Kokkini, S., R. Karousou, and T. Lanaras.1995. Essential oils of Spearmint (carvone-rich) plants from the Island of Crete (Greece). Biochem. Syst. Ecol. 23: 287- 297.

Jan, S., M. A. Khan, S. U. din, W. Murad, M. Hussain, and A. Ghani. 2008. Herbal remidies used for gastrointestinal disorders in Kaghan Valley, N.W.F.P, Pakistan. Pak. J. Weed Sci. Res. 14: 169-200.

Hedge, I. C. 1994. Labiatae. In: S. I. Ali, and Y. J. Nasir (Eds.) Flora of Pakistan. Department of Botany, University of Karachi 192: 255-264.

Harley, R. M., and C. A. Brighton. 1977. Chromosome radioprotection: a radiation-induced conditioned taste aversion study. Phytotherapy Research 23: 293-296.

Haq, F. H. Ahmad, M. Alam, and Rahatullah. 2010. Species diversity of vascular plants of Nandyar Valley Western Hamalaya Pakistan. Pak. J. Bot. 42: 213-229.

Gobert, V., S. Moja, M. Colson, and P. Taberlet. 2002. Hybridization in the section Mentha (Lamiaceae) inferred from AFLP markers. American Journal of Botany 89: 2017-2023.

Doyle, J. J., and J. L. Doyle. 1990. Isolation of plant DNA from fresh tissue. Focus 12: 13-15.

Dorman, H. J., M. Kosar, K. Kahlos, Y. Holm, and R. Hiltunen. 2003. Antioxidant prosperities and composition of aqueous extracts from Mentha species, hybrids, varieties and cultivars. J. Agric. Food Chem. 51: 4563-4569.

Celenk, S., G. Tarimcilar, A. Bicakci, G. Kaynak, and H. Malyer. 2008. A palynological study of the genus Mentha (Lamiaceae). Botanical Journal of the Linnean Society 157: 141–154.

Broza, Š. K., F. Silvia, D. Christoph, O. Silvester, T. Daniela, G. Daniel, R. Gottfried, and S. Johannes. 2009. Multivariate Numerical Taxonomy of Mentha Species, Hybrids, Varieties and Cultivars. Sci. Pharm. 77: 851–876.

Bowles, E. J. 2003. The chemistry of aromatherapeutic oils, Allen and Unwin, Crow’s Nest, NSW, Australia, pp. 256.

Betts, T. J. 2001. Chemical characterization of the different types of volatile oil constituents by various solute retention ratios with the use of conventional and novel commercial gas chromatographic stationary phases. J. Chromt. A. 936: 33-46.

Anonymous. 1996. Computer package MINITAB. Minitab Inc. 13.5 for windows 1995. SPSS, Inc., Chicago.

Angioni, A., A. Barra, V. Coroneo, S. Dessi, and P. Cabras. 2006. Chemical composition, Antimicrobial and antioxidant activities of three Mentha species antioxidant activities of Tunisian halophytes. Compte Rendues antioxidant potential of pudina extract. J. Agric. Food Chem. 54: 4364-4370.

Ali, S. I., and Y. J. Nasir. 1990. Labiatae: Flora of West Pakistan. University of Karachi.

Abid, R., and M. Qaiser. 2006. Numerical Analysis of Inula and its Allied Genera from Pakistan and Kashmir. Pak. J. Bot. 38: 521-526

Abbott, L. A., F. A. Bisby, and D. J. Rogers. 1985. Taxonomic analysis in biology. Computers, models and databases. Columbia.

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