HASAN PINAR, Übeyit SEDAY, Aydın UZUN, MUSTAFA ÜNLÜ, MEHMET YAMAN

Bazı Önemli Turunçgil Anaçları ile Melezlerinde Citrus Tristeza Virus (CTV)'ne Dayanıklılığın SCAR Markırları ile Belirlenmesi

Citrus tristeza virus (CTV), dünyada turunçgillerde özellikle turunç anacı (Citrus aurantium L.)üzerine aşılı ağaçlarda hızlı bir şekilde ölüme sebep olan en önemli viral patojendir. CTV'yedayanıklı çeşitlerin geliştirilmesi turunçgil ıslah programlarının önceliği haline gelmiştir. Genetikçalışmalarda, Üç Yapraklı'da (Poncirus trifoliata), dayanıklılığı kontrol eden dominant tek bir genbelirlenmiştir. Ayrıca önceki çalışmalarla CTV'ye dayanıklılık için tek bir dominant gen ile ilişkili 11dominant RAPD (Randomly Amplified Polymorphic DNA) fragmenti tanımlanmıştır. Bunların yedisiklonlanmış ve SCAR (Sequence-Characterized Amplified Region)markırına dönüştürülmüştür.Bunlardan üçü SC007, SCAD08 ve SCTO8 markırlarıdır. Bu çalışmada 14 turunçgil anacı(Volkameriana, Kabalimon, Kleopatra, Citrumelo, Troyer, Taiwanica, Carrizo, Brezilya Turuncu,Yerli Turunç, Üç Yapraklı, Yuzu, Rangpur lime, Şadok ve Fortunella spp.) ile 48 adet Turunç X ÜçYapraklı ve 21 adet Kabalimon X Üç Yapraklı melezleri CTV dayanımı bakımından 3 SCAR markırı(SCAD08, SC007, SCTO8) ile taranmıştır. SCAD08 ve SCTO8 markırları ile polimorfizm eldeedilirken SCO07 markırı ile melez genotiplerde polimorfizm elde edilememiştir. Çalışmadan eldeedilen bulgulur söz konusu SCAR markırlarının büyük populasyonlarda kullanılarak dayanıklılıkbandı elde edilen bireylerde klasik testlemeyle teyit edilmesi durumunda kullanılabileceğinigöstermektedir. Bu yolla hastalıkla ilgili ıslah çalışmalarında sürenin kısaltılması mümkünolabilecektir

Resistance of Some Important Citrus Rootstocks and Their Hybrids to Citrus Tristeza Virus (CTV) through SCAR Markers

Citrus tristeza virus (CTV) is the most significant viral pathogen leading rapid deaths in citrus treesgrafted on Sour orange (Citrus aurantium L.) rootstocks. Therefore, development of CTV-resistantcultivars has become the primary target of citrus breeding programs. In previous genetic work carriedout upto now, a single dominant gene controlling the resistance in Poncirus (Poncirus trifoliata) hasbeen identified. Together with a single dominant gene for resistance to CTV, 11 related dominantRAPD (Randomly Amplified Polymorphic DNA) fragments have been defined. Of these fragments,seven were cloned and transformed into SCAR (Sequence-Characterized Amplified Region) markers(three of which are SC007, SCAD08 and SCTO8). In this study, 14 citrus rootstocks (Volkameriana,Kabalimon, Kleopatra, Citrumelo, Troyer, Taiwanica, Carrizo, Brezillian Sour orange, Local SourOrange, P. trifoliata, Yuzu, Rangpur lime, Şadok and Fortunella spp.) and 48 Sour Orange X P.Trifoliata and 21 Rough lemon X P. trifoliata hybrids were screened for resistance to CTV withpreviously developed 3 SCAR markers (SCAD08, SC007, SCTO8). While polymorphism wasobserved with SCAD08 and SCTO8 markers, polymorphism was not observed with SCO07 markersin hybrid genotypes. Current findings revealed that these SCAR primers could be used only providedthat they were used in large populations and verified through classical testing over individuals withresistance bands. In this way, it is possible to shorten the durations of breeding works for the diseases

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Arumuganathan, K., Earle E.D., 1991. Nuclear DNA content of some important plant species. Plant Molecular Biology Reporter, 9(3):208-219.
Ayazpour, K., Sijam, K., Vadamalai, G., Jaafar, H., 2011. Pomelo, a resistance variety to Citrus tristeza virus in peninsular Malaysia.2nd International Conference on Biotechnology and Food Science, IPCBEE vol.7.
Baloglu, S., Birisik, N., 2009. Historical review ofCitrus tristeza virüs (CTV) and its vectors in Turkey. Méditerranéennes, Series B.65 Citrus tristeza virus and Toxoptera citricidus: a serious threat to the Mediterranean citrus industry. CIHEAM-IAMB; Bari, Italy: pp. 85-88.
Bar-Joseph, M., Yang, G., Gafny, R., Mawassi, M., 1987. Subgenomic RNAs: the possible building blocks for modular recombination of closterovirus genomes Semin. Virol, 8 (1997), pp. 113-119.
Bernet, G.P. , Bretó M. P. , Asins M.J., 2004. Expressed sequence enrichment for candidate gene analysis of citrus tristeza virus resistance.Theoretical and Applied Genetics February 2004,Volume 108,Issue 4,pp 592-602.
Dambier, D., Benyahia, H., Pensabene-Bellavia, G., Kaçar, Y.A., Froelicher, Y., Belfalah, Z., Yesiloglu, T., 2011. Somatic hybridization for citrus rootstock breeding: an effective tool to solve some important issues of the Mediterranean citrus industry. Plant cell reports,30(5), 883-900.
Deng, L., Wang, C., Spencer, E., Yang, L., Braun, A., You, J., Slaughter, C., Pickart, C., Chen, Z.J., 2000. Activation of the I?B kinase complex by TRAF6 requires a dimeric ubiquitin-conjugating enzyme complex and a unique polyubiquitin chain.Cell,103:351- 361.
Deng, Z., Huang, S., Xiao, S.Y., Gmitter F.G., 1997. Development and characterization of SCAR markers linked to the citrus tristeza virus resistance gene from Poncirus trifoliata. Genome,40:697-704.
Fang, D.Q., Federici, C.T., Roose, M.L., 1998. A high resolution linkage map of the citrus tristeza virus resistance gene region in Poncirus trifoliata (L.) Raf. Genetics, 150:883- 890.
Fang, D.Q., Roose, M.L., 1999. Inheritance of intersimple sequence repeat markers in citrus. Journal of Heredity, 90, 247-249.
Garnsey, S.M., Barrett, H.C., Hutchinson, D.J., 1987. Identification of citrus tristeza virus resistance in citrus relatives & its potential applications. Phytophylactica, Vol. 19, pp. 187-191.
Garnsey, S.M., Su, H.J., Tsai, M.C., 1997. Differential susceptibility of pummelo and Swingle citrumelo to isolates of citrus tristeza virüs.P,138-146. In: Da Graca, J.V., P. Moreno and R.K. Yokomi (eds.). Proc. 13th Conf. Intl. Organ. Citrus Virologists, Univ. California Press, Riverside, Calif.
Gmitter, F.G., Xiao, S.Y., Huang, S., Hu, X.L., Garnsey, S.M., Deng, Z., 1996. A localized linkage map of the citrus tristeza virus resistance gene region. Theor. Appl. Genet,92:688-695.
Grill, E., Somerville, C., 1991.Construction and characterization of a yeast artificial chromosome library of Arabidopsis which is suitable for chromosome walking. Mo/. Gen. Genet, 226, 484-490.
Kim, E., Hammond-Kosack Jonathan D., Jones, G., 1997. Resistance gene-dependent plant defense responses. The Plant Cell, Vol. 8, 1773-1791.
Lande,R.,Thompson, R., 1990. Efficiency of marker-assisted selection in the improvement of quantitative traits.GENETICS,vol. 124 no. 3 743-756.
Martin, G.B., Brommonschenkel, S.H., Chunwongse, J., Frary, A., Ganal, M.W., Spivey, R., Wu, T., Earle, E.D., Tanksley, S.D., 1993. Map-based cloning of a protein kinase gene conferring disease resistance in tomato. Science www.plant.org, e 262: 1432-1436.
Martin, G.B., Frary, A., Wu, T., Brommonschenkel, S.H., Earle, E.D., Tanksley, S.D., 1994. A member of the tomato Pto gene family confers sensitivity to fenthion resulting in rapid cell death. Plant Cell, 6 1543-1552.
Mestre, P.F., Asins, M.J., Pina, J.A., Navarro, L., 1997. Efficient search for new resistant genotypes to the citrus tristeza closterovirus in the orange subfamily Aurantioideae. Theor. Appl. Genet., 95:1282-1288.
Michelmore, R.W., Meyers, B.C., 1998.Clusters of resistance genes in plants evolve by divergent selection and a birth-and-death process. Genome Research,8:1113-1130.
Moreno, P., Ambrós, S., Albiach-Martí, M.R., Guerri, J., Peña, L., 2008. Citrus tristeza virus: a pathogen that changed the course of the citrus industry. Molecular Plant Pathology, 9(2), 251-268.
Nelson, C.J.,Wang, S., Wu, Y., Li, X., Antony, G., White, F.F., Yu, J., 2011. Single-nucleotide polymorphism discovery by high-throughput sequencing in sorghum.BMC Genomics, 12:352.
Roistacher, C.N., Da Graça, J., 2006. Difficult times. In: IOCV Newsletter, April 2006, 1.
Roistacher, C.N., 1991. Techniques for biological detection of specific citrus graft-transmissible diseases. In: Roistacher C.N. (ed.), Graft-transmissible Diseases of Citrus. Handbook for Detection and Diagnosis. Rome, FAO: 35-45.
Yang, P., Mlynczak, M.G., Wei, H., Kratz, D.P., Baum, B.A., Hu, Y.X., Wiscombe, W.J., Heidinger, A., Mishchenko, M.I., 2003. Spectral signature of ice clouds in the far- infrared region: Single-scattering calculations and radiative sensitivity study.J. Geophys. Re, 108, No. D18, 4569.
Yokomi, R.K., Lastra, R., Stoetzel, M.B., Damsteegt, V.C., Lee, R.F., Garnsey, S.M., Gottwald, T.R., Rocha Peaa, M.A., Niblett, C.L., 1994. Establishment of the brown citrus aphid (Homoptera: Aphididae) in Central America and the Caribbean Basin and transmission of citrus tristeza virus. Journal of Economic Entomology, 87: 1078-1085.
Yoshida, T., 1985. Inheritance of susceptibility to citrus tristeza virus in trifoliate orange (in Japanese with English summary). Bul. Fruit Tree Res. Sta. (Okitsu) B, No.12. p. 17-26.