Akciğer Tüberkülozlu Hastaların Bronkoalveolar Lavajında İL-12, İFN-γ ve sİL-2 Düzeyleri
Amaç: Çalışmamızda lokal immün cevabı yansıttığı düşünülen sitokin seviyelerini (İL-12, İFN-γ vesİL-2) bronkoalveolar lavaj sıvısında ölçerek balgamda yayma negatif saptanan hastalar ile balgamyayması pozitif olan hastaları karşılaştırmayı amaçladık.Gereç ve Yöntemler: Çalışma retrospektif olarak dizayn edildi. Çalışmaya Ekim 1998-Şubat 1999arasında Yedikule Göğüs Hastalıkları ve Göğüs Cerrahisi Eğitim ve Araştırma Hastanesi’nde tetkik vetedavi edilen 24 tüberküloz hastası alındı. Kontrol grubu olarak diğer nedenlerle bronkoskopi yapılmışolan akciğer malignitesi veya tüberkülozu olmayan 15 hasta alındı.Bulgular: Hasta ve kontrol grupları İL-12, İFN-γ ve sİL-2 düzeyleri açısından karşılaştırıldığındaİL-12 ve İFN-γ düzeylerinin anlamlı şekilde farklı olduğu tespit edildi (sırasıyla p: 0,02 ve p
IL-12, IFN-γ and sIL-2 Levels in Bronchoalveolar Lavage of Patients with Pulmonary Tuberculosis
Objective: We aimed to compare cytokine levels (IL-12, IFN-γ and IL-2), thought to reflect local immunologic response, in sputum smear positive and negative patients by measuring them in bronchoalveolar lavage fluid in this study. Material and Methods: The study had a retrospective design. A total of 24 tuberculosis patients who were examined and treated at Yedikule Chest Diseases and Chest Surgery Training and Research Hospital between October 1998 and February 1999 were included. Patients without pulmonary malignancy and tuberculosis who had undergone bronchoscopy for other reasons were included as the control group. Results: When the IL-12, IFN-γ and sIL-2 levels were compared in the patient and control groups, the IL-12 and IFN-γ levels were found to show a significant difference (p: 0.02 and p
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- Wolf SF, Temple Pa, Kobayashi M, Young D, Dicig
M, Lowe L, Dzialo R, Fitz L, Ferenz C, Hewick RM.
Cloning of cDNA for natural killer cell stimulatory factor, a
heterodimeric cytokine with multiple biologic effects on T
and natural killer cells. J Immunol 1991; 146(9):3074-81.
- Gubler U, Chua AO, Schoenhaut DS, Dwyer CM,
McComas W, Motyka R, Nabavi N, Wolitzky AG,
Quinn PM, Familletti PC. Coexpression of two distinct
genes is required to generate secreted bioactive cytotoxic
lymphocyte maturation factor. Proc Natl Acad Sci USA.
1991; 88(10):4143-7.
- Boom WH, Toossi Z, Wolf SF. The modulation by IL2,
NKSF (IL-12/CLMF) and TGF-Bof CD4+ T cell
mediated cytotoxity for macrophages (abstract). In Proc
Twenty-Seventh Joint Conf Tuberc Lepr 1992;163-7.
- Zhang M, Gately MK, Wang E, Gong J, Wolf SF, Lu S,
Modlin RL, Barnes PF. Interleukin 12 at the site of disease
in tuberculosis. J Clin Invest 1994; 93(4):1733-9.
- Nikaido T, Shimizu N, Ishida N, Sabe H, Teshigawara
K, Maeda M, Uchiyama T, Yodoi J, Honjo T. Molecular
cloning of cDNA encoding human IL-2 receptor. Nature
1984; 311:631-5.
- Panek SJ, Boer JS. Diagnosis of pulmonary tuberculosis
by flexible fiberoptic bienchoscopy. Am Rev Respir Dis
1979; 119:677-9.
- Chan HS, Sun AJ, Hoheisel GB. Bronchoscopic aspiration
and Bronchoalveolar lavage in diagnosis of sputum smearnegative
pulmonary tuberculosis. Lung 1990; 168:215-20.
- Dalton DK, Pitts-Meek S, Keshav S, Figari IS, Bradley
A, Stewart TA. Multiple defect of immune cell function
in mice with disrupted γ-interferon genes. Science 1993;
259:1739-42.
- Huang S, Hendriks W, Althage A, Hemmi S, Bluethmann
H, Kamijo R, Vilcek J, Zinkernagel RM, Aguet M.
Immune response in mice that lack the interferon-γ
receptor. Science 1993; 259:1742-5.
- Robinson DS, Ying S, Taylor IK, Wangoo A, Mitchell
DM, Kay AB, Hamid Q, Shaw RJ. Evidence for a Th1-
like bronchoalveolar T-cell subset and predominance
of interferon-gamma gene activation in pulmonary
tuberculosis. Am J Respir Crit Care Med 1994; 149:989-
93.
- Condos R, Rom WN, Liu YM, Schluger NW. Local
immune responses correlate with presentation and
outcome in tuberculosis. Am J Respir Crit Care Med
1998; 157:729-35.
- Taha RA, Kotsimbos TC, Song YL, Menzies D, Hamid
Q. IFN-gamma and IL-12 are increased in active
compared with inactive tuberculosis. Am J Respir Crit
Care Med 1997; 155:1135-9.
- Liu X, Hou X-F, Gao L,Deng GF, Zhang MX, Deng QY,
Ye TS, Yang QT, Zhou BP, Wen ZH, Liu HY, Kornfeld
H, Chen XC. Indicators for prediction of Mycobacterium
tuberculosis positivity detected with bronchoalveolar
lavage fluid. Infectious Diseases of Poverty 2018; 7:22.
- Küpeli E, Karnak D, Beder S, Kayacan O, Tutkak H.
Diagnostic accuracy of cytokine levels (TNF-alpha,
IL-2 and IFN-gamma) in bronchoalveolar lavage fluid
of smear-negative pulmonary tuberculosis patients.
Respiration 2008; 75(1):73-8. Epub 2007 Nov 1.
- Taha RA, Minshall EM, Olivenstein R, Ihaku D, Wallaert
B, Tsicopoulos A, Tonnel AB, Damia R, Menzies D,
Hamid QA. Increased expression of IL-12 receptor
mRNA in active pulmonary tuberculosis and sarcoidosis.
Am J Respir Crit Care Med 1999; 160:1119-23.
- Nolan A, Fajardo E, Huie ML, Condos R, Pooran A,
Dawson R, Dheda K, Bateman E, Rom WN, Weiden
MD. Increased Production of IL-4 and IL-12p40
from bronchoalveolar lavage cells are biomarkers of
Mycobacterium tuberculosis in the Sputum. PLoS ONE 2013;
8(3):e59461.
- Somoskovi A, Zissel G, Zipfel PF, Ziegenhagen MW,
Klaucke J, Haas H, Schlaak M, Müller-Quernheim J.
Different cytokine patterns correlate with the extension
of disease in pulmonary tuberculosis. Eur Cytokine Netw
1999; 10:135-42.
- Pushpakom R, Ong-Ajyooth S, Bovornkiti S. The
association of adenosine deaminase activity with
T-lymphocytes and subsets in pulmonary tuberculosis and
bronchogenic carcinoma. J Med Assoc Thai 1990; 73:1-11.
- Tchou-Wong KM, Tanabe O, Chi C, Yie TA, Rom WN.
Activation of NK-kappaB in Mycobacterium tuberculosisinduced
interleukin-2receptor expression in mononuclear
phagocytes. Am J Respir Crit Care Med 1999; 159:1323-9.