Ebru BATI AY, Şevket Metin KARA, Muhammed Akif AÇIKGÖZ, Sedanur ODABAŞ

Işığın Echinacea purpurea L. kallus kültüründe alkamide, kafeik asit türevleri ve echinacoside biyosentezi üzerine etkisi

Işık sadece fotosentez, büyüme ve gelişmede etkili değildir, aynı zamanda birincil ve ikincil metabolizma ürünlerinin biyosentezinde de önemli bir rol oynamaktadır. Bu araştırmanın amacı ışığın Echinacea purpurea L. hücre süspansiyon kültüründe alkamide, kafeik asit türevleri ve echinacoside biyosentezine etkisini tespit etmektir. Steril bitkilerin gövde eksplantlarından kalluslar elde edilmiş ve 1.0 mg/L BAP ve 2.0 mg/L NAA ilave edilmiş B5 ortamında kültüre alınmışlardır. Daha sonra, 8 günlük hücre kültürleri, aydınlık ve karanlık ortamlarda inkübe edilmiştir ve hücreler, üç günde bir olmak üzere, toplamda beş kez hasat edilmiştir. Alkamide, kafeik asit türevleri ve echinacoside içerikleri HPLC ile belirlenmiştir. Alkamide, kaftarik asit ve echinacoside içeriği ışık uygulamalarıyla düzenli olarak artmıştır. Işık uygulaması ile alkamide içeriği karanlık ortama göre ortalama olarak %57 artmıştır. En yüksek alkamide birikimi ışıkta 12 günlük inkübasyona maruz bırakılan hücre kültüründe tespit edilmiş ve karanlık ortama göre 2.11 katlık bir artış gerçekleşmiştir. Hücre kültürlerinin 12 gün süreyle ışığa maruz bırakılması, kaftarik asit ve echinacoside birikiminde %70’lik ve %63'lük bir artışa yol açmıştır. Bu araştırma, ışık uygulamasının Echinacea purpurea L. hücre süspansiyon kültüründe bazı önemli fitokim yasalların artırılmasında büyük bir potansiyele sahip olduğunu göstermiştir.

Effect of light on biosynthesis of alkamide, caffeic acid derivatives and echinacoside in Echinacea purpurea L. callus cultures

Light is not only effective in photosynthesis, growthand development, but also play an important role inbiosynthesis of primary and secondary metabolites.The present research was aimed to determine effectof light on biosynthesis of alkamide, caffeic acid derivatives and echinacoside in cell suspension cultures of Echinacea purpurea L. Stem explants derivedfrom sterile plants were subjected to callus culture ina B5 media supplemented with 1.0 mg/L BAP and2.0 mg/L NAA. Afterward, 8-day cell cultures wereincubated in light and dark environments and cellharvesting, with a three-day interval, was carried outfive times. The contents of alkamide, caffeic acidderivatives and echinacoside were determined usingHPLC. Alkamide, caftaric acid and echinacoside content increased regularly with light application. Lightapplication increased alkamide content by 57%, asan average, compared to darkness. The cell culturesexposed to 12 days of incubation in light producedthe highest alkamide content, with a 2.11-fold increase, compared to dark. Subjecting the cell culturesto light for 12 days resulted in 70% and 63% increases in caftaric acid and echinacoside. This studyshowed that light application in Echinacea purpureaL. cell suspension culture had a great potential forincreasing some important phytochemicals.

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