Fundagül EREM, Muharrem CERTEL, Barçın KARAKAŞ BUDAK

Sünme (Rope) Problemi Olan Ekmeklerden İzole Edilen Bacillus Türlerinin Enterotoksin Üretme Potansiyeli

Bu çalışmada, daha önceki bir çalışmamızda sünme (rope) hastalığı oluşmuş ekmek içlerinden izole edilen Bacillustürleri, hemolitik (Hbl) ve hemolitik olmayan (Nhe) enterotoksin üretimleri açısından incelenmiştir. İncelenen toplam 39izolattan sadece birinin hem Hbl hem de Nhe enterotoksinlerini, 22 izolatın sadece Hbl, 2 izolatın ise sadece Nheenterotoksinini üretme kapasitesinin olduğu, kalan 14 izolatın ise her iki enterotoksini de üretmedikleri tespit edilmiştir.Enterotoksin analizleri için pozitif kontrol suşu olarak, her iki enterotoksini de ürettiği bilinen Bacillus cereus 2248kullanılmıştır. Toksin analizleri, standart suşlar olan Bacillus subtilis PY22, B. subtilis RSK 244 ve B. subtilis RSK 246için de yapılmış, aralarından sadece B. subtilis RSK 246’nın Nhe enterotoksinini üretme potansiyelinin olduğubelirlenmiştir. Ayrıca emetik toksin ürettiği bilinen Bacillus cereus 2455/2 suşu da Nhe enterotoksini açısından pozitifsonuç vermiştir. Sonuçlar, ekmeklerde sünme (rope) hastalığından sorumlu olan Bacillus türlerinin enterotoksinüretebileceğini gösterir niteliktedir.

Enterotoxin Production Potential of Bacillus Species Isolated from Ropy Bread

In this study, Bacillus species isolated from ropy bread crumb in our previous study were investigated for the production of hemolytic (Hbl) and non-hemolytic (Nhe) enterotoxins. Of the 39 isolates, only one produced both Hbl and Nhe enterotoxins, 22 produced only Hbl, 2 produced only Nhe and the remaining 14 isolates were found to produce none of the two enterotoxins. Bacillus cereus 2248, which is known to produce both Hbl and Nhe, was used as the positive control strain for the enterotoxin assays. Toxin assays were also performed for the standard strains Bacillus subtilis PY22, B. subtilis RSK 244 and B. subtilis RSK 246. It was determined that amongst these strains only B. subtilis RSK 246 has the potential of producing the Nhe enterotoxin. Furthermore, the test results of Bacillus cereus 2455/2, which is known as an emetic strain, have shown that the strain was potentially positive in terms of Nhe enterotoxin. The results showed that Bacillus strains that are responsible for rope disease in bread could produce enterotoxins.

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