Arda ACEMİ, Yonca Avcı DUMAN, Fazıl ÖZEN, Yonca YÜZÜGÜLLÜ KARAKUŞ, Halil İ. TOYGAR

UV-C Stresinin 6-Benzilaminopürin Varlığında Çoğaltılmış Amsonia orientalis’in In vitro Sürgün Gelişimi ve Antioksidan Enzimleri Üzerine Etkileri

Bu çalışmada abiyotik faktörlerden biri olan UV-C stresinin 6-benzilaminopürin varlığında çoğaltılmış Amsonia orientalis’in in vitro sürgünleri üzerindeki etkileri, sürgünlerin morfometrik özellikleri, H2O2 (hidrojen peroksit) ve MDA (malondialdehit) seviyeleri ile antioksidan enzimleri analiz edilerek irdelenmiştir. Test edilen 3,47 (15 dk), 6,94 (30 dk) ve 13,87 (60 dk) kJ m-2’lik UV-C radyasyon dozlarının hepsi sürgün uzunluğu ile sürgün sayısında kontrole (UV-C uygulanmamış bitki) oranla düşüşe sebep olmuştur. Radyasyon kaynaklı oluşan bu stres 6-benzilaminopürin varlığında sürgün gelişimini yavaşlatsa da tamamen durdurmamıştır. UV-C radyasyon uygulamaları antioksidan enzimler üzerine farklı etkiler göstermiştir. SOD (süperoksit dismutaz) aktivitesi artan radyasyon dozuna bağlı olarak kontrole göre önemli ölçüde azalırken, POD (peroksidaz) aktivitesinde artış gözlenmiştir. CAT (katalaz) aktivitesinin ise radyasyondan önemli ölçüde etkilenmediği görülmüştür. Artan UV-C dozlarında H2O2 ve MDA seviyelerinin benzer olarak arttığı bulunmuştur. Elektroforetik analizler sonucunda SOD enzimi için üç farklı izozim (Cu/Zn-SOD, Fe-SOD ve Mn-SOD) tespit edilmiş olup, artan UV-C stresinin bitkinin Cu/Zn-SOD aktivitesini tamamen inhibe ettiği gözlenmiştir. Sonuçlar bitkinin UV stresine karşı gösterdiği savunma mekanizmasında peroksidazların incelenen diğer antioksidan enzimlere göre öncelikli rol aldığına işaret etmektedir.

The Effects of UV-C Stress on In vitro Shoot Development and Antioxidant Enzymes of Amsonia orientalis Cultured in the Presence of 6-Benzylaminopurine

In this study, the effects of UV-C stress –being one of the abiotic stress factors– on in vitro shoot development and antioxidant enzymes of Amsonia orientalis cultured in the presence of 6benzylaminopuine were examined based on the analysis of morphometric parameters, antioxidant enzymes, MDA (malondialdehyde) and H2O2 contents of in vitro shoots. All tested UV-C radiation dosages; 3.47 (15 min), 6.94 (30 min) and 13.87 (60 min) kJ m-2 reduced shoot length and shoot number compared to the control (non UV-C-applied). In the presence of 6-benzyaminopurine, radiation-sourced stress decreased shoot development but did not totally inhibit. UV-C radiation exerted different effects on antioxidant enzymes. It was found that SOD (superoxide dismutase) activity was reduced with respect to increasing radiation doses while POD peroxidase activity was enhanced. CAT activity was not significantly affected by radiation. Long-term UV-C exposure has been shown to increase similarly both H2O2 and MDA levels. Electrophoretic analysis showed that there are three SOD isozymes (Cu/Zn-SOD, Fe-SOD ve Mn-SOD) found and Cu/Zn-SOD was totally inhibited under all UV-C treatments. The results indicate that peroxidases play more important role than the other antioxidant enzymes in the plant’s defense mechanism against UV stress.

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