Elanur AYDIN, Fazıl HACIMÜFTÜOĞLU, Hasan TÜRKEZ

Silikon Nanopartikül Maruziyetine Bağlı Olarak Oluşan İn Vitro Sitotoksik ve Moleküler Etkiler

Silikon nanopartikülleri çevresel, biyolojik, kimyasal ve fiziksel amaçlarla çeşitli alanlarda yaygın olarak kullanılmaktadır. Bu nanopartiküllerin klinik ve endüstriyel alanlarda kullanılabilmesi için güvenilirlikleri özellikle genotoksisite ve sitotoksisite açısından doğrulanmalıdır. Bu yüzden mevcut çalışmada, yaygın olarak kullanılan silikon nanopartiküllerinin ( silikon karbid, silikon dioksit, silikon nitrit) insan alveolar epitel (HPAEpiC) ve farinks (HPPC) hücrelerindeki sitotoksisitesi ve gen ekspresyon profillerindeki değişimlerin araştırılması amaçlanmıştır. HPAEpiC ve HPPC hücreleri 72 saat boyunca silikon nanopartikülleriyle (0-100 µg/mL) muamele edildi. Nanopartiküllerin sitotoksisite değerlendirmeleri için 3-(4,5 dimetylthiazol -2-yl) - 2,5 diphenltetrazolium bromide (MTT) ve laktat dehidrogenaz salınım (LDH) yöntemleri kullanılırken; genotoksisite analizi için cDNA array - RT-PCR yöntemi kullanıldı. MTT ve LDH yöntemi sonuçlarına göre, uygulanan bütün test nanopartikülleri hem HPAEpiC hem de HPPC hücre hatlarında doza bağlı olarak sitotoksisiteyi indüklemiştir. HPAEpiC ve HPPC hücrelerinin ilgili genler açısından (apoptozis, DNA fasarı ve tamiri, oksidatif stres) gen ekspresyon profilleri incelendiğinde silikon naopartiküllerinin ekspresyonu değiştirdiği gözlenmiştir. Bu çalışmadan elde edilen nanotoksisiteye bağlı olarak oluşan gen ekspresyon profilleri, silikon nanopartiküllerin klinik ve çevresel uygulamalarda kullanılabilmesi için önemli bir kaynak oluşturmaktadır

In Vitro Cytotoxicity and Molecular Effects Related to Silicon Nanoparticles Exposures,

Silicon nanoparticles are widely used for various applications including environmental, biological, chemical and physical. And, to translate these nanomaterials to the clinic and industrial domains, their safety needs to be verified, particularly in terms of genotoxicity and cytotoxicity. Therefore, in this study, we aimed to investigate of cytotoxicity and changes in gene expression profiles influenced by commonly silicon (as silicon carbide, silicon dioxide, silicon nitride) nanoparticles in human alveolar epithelial (HPAEpiC) and pharynx (HPPC) cell lines in vitro since inhalation is an important pathway for exposure to these nanoparticles. HPAEpiC and HPPC cells were treated with silicon (0-100 µg/mL), nanoparticles for 72 h, and then cytotoxicity was detected by, [3-(4,5-dimethyl-thiazol-2-yl) 2,5diphenyltetrazolium bromide] (MTT) and lactate dehydrogenase (LDH) release assays, while genotoxicity was also analyzed by cDNA array - RT-PCR assay. According to the results of MTT and LDH assays, all tested nanoparticles induced cytotoxicity on both HPAEpiC and HPPC cells in dose-dependent manner. Determining and analyzing the gene expression profiles of HPAEpiC and HPPC cells, silicon nanoparticles showed changes in genes related to apoptosis, DNA damage or repair and oxidative stress. This study of gene expression profiles affected by nanotoxicity provides critical information for the clinical and environmental applications of silicon nanoparticles

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