Ufuk YILDIZ

Kükürt İçeren Ligand ve Bakır Kompleksine Ait Antioksidan ve Topoizomeraz I İnhibitör Aktivitelerin Karşılaştırılması

Bu çalışmada antikanser ve antioksidan ajan olarak kullanılabilecek dtb ligandı ve bu ligantın[Cu(dtb)(phen)]2+ kompleksi ile çalışılmıştır. Antioksidan aktivitelerinin belirlenmesi için DPPHradikali süpürme yöntemi kullanılmıştır. Dtb ligandı düşük antioksidan aktivite gösterirken buligantın Cu(II) kompleksinin iyi bilinen bir antioksidan olan C vitamini kadar yüksek antioksidanaktivite gösterdiği görülmüştür. Topoizomeraz-I inhibisyonu aktivitesi çalışmasında pBR322plazmid DNA’sının relaksasyonu agaroz jel elektroforez yöntemiyle takip edilmiştir. Antioksidanaktivitede olduğu gibi topoizomeraz-I inhibisyonu aktivitesinde de dtb ligandı düşük etkigösterirken bakır kompleksi μM gibi düşük derişimde bile inhibisyon gerçekleştirebilmiştir.[Cu(dtb)(phen)]2+ kompleksinin ileri in-vivo ve in-vitro antikanser çalışmalarının yapılması halindeantikanser ve antioksidan ajan olarak kullanılabilme potansiyelinin yüksek olduğu görülmüştür.

Comparison of Antioxidant and Topoisomerase I Inhibitor Activities of Sulfur Containing Ligand and Copper Complex

In this study, dtb ligand and its [Cu (dtb) (phen)]2+ complex which can be used as anticancer and antioxidant agent were studied. DPPH radical scavenging method was used to determine the antioxidant activities. The dtb ligand had low antioxidant activity, while the Cu (II) complex of this ligand had as high antioxidant activity as vitamin C, a well-known antioxidant. In the topoisomerase-I inhibition activity study, relaxation of pBR322 plasmid DNA was followed by agarose gel electrophoresis method. In addition to antioxidant activity, dtb ligand had a low effect on topoisomerase-I inhibition activity, while the copper complex was able to inhibit even at low concentrations such as 10 μM. The obtained results show [Cu (dtb) (phen)]2+ complex has a high potential to be used as an anticancer and antioxidant agent.

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