Glioblastoma Multiformede Friedelinin Antikanser Aktivitesinin In Vitro İncelenmesi
Pek çok kanser hastalığında destek için kullanılan farklı bitkisel yöntemler vardır. Likenler benzersiz bitkisel bileşikler içeren önemli organizmalardır ve farklı antikanser aktivitelerine sahip oldukları bilinmektedir. Bu özelliklerden yola çıkarak, mevcut çalışma, beyin kanseri türleri içerisinde tehlikeli habis özellikler gösteren glioblastoma multiformeye (GBM) karşı bir liken bileşiği olan friedelinin (FRI) antikanser aktivitesini araştırmayı amaçlamıştır. FRI'nın yan etki düzeyini belirlemek için insan U87MG- GBM kanser hücre hatları ve Sprague-Dawley sıçanlarından izole edilen primer sıçan serebral korteks (PSSK) kanserli olmayan hücreler kullanılmıştır. Denemelerde, FRI'nın farklı konsantrasyonlarının sitotoksik (3-(4,5-dimetiltiazol-2-il)-2,5-difeniltetrazolyum bromür (MTT) ve laktat dehidrogenaz (LDH) testleri ile), antioksidan (toplam antioksidan kapasite (TAK) testi ile), pro-oksidan (toplam oksidatif stres (TOS) ile) ve genotoksik (8-hidroksi-2′-deoksiguanozin (8-OH-dG) testi ile) aktiviteleri test edilmiştir. Çalışma sonucunda MTT uygulaması, FRI'nın PSSK hücrelerine kıyasla U87MG hücreleri üzerinde daha yüksek sitotoksik aktivite gösterdiğini ortaya koymuştur (sırasıyla medyan inhibitör konsantrasyonu (IC 50 ): 1271.77 ve 46.38 mg/L). U87MG hücreleri temel alındığında, LDH ve TOS aktiviteleri arasında anlamlı bir pozitif korelasyon olduğu saptanmıştır. Sağlıklı PSSK hücrelerinde, TAK ve hücre canlılığı arasındaki yüksek pozitif korelasyon FRI'nın antioksidan kapasitesini sergilemiştir. Sonuç olarak, mevcut çalışmadan elde edilen sonuçlar, GBM'ye karşı antikanser bileşiği kaynağı olarak antioksidan kapasiteye sahip doğal ürün potansiyelini ortaya koymuştur.
In Vitro Investigation of the Anticancer Activity of Friedelin in Glioblastoma Multiforme
There are different herbal methods used for support in many cancer diseases. Lichens are importantorganisms containing unique herbal compounds and it is known that they have different anticanceractivities. Starting from these features, the present study was aimed to investigate anticancer activity offriedelin (FRI), a lichen compound against glioblastoma multiforme (GBM) showed dangerous malignantproperties within brain cancer species. It was used human U87MG-GBM cancer cell lines and primaryrat cerebral cortex (PRCC) non-cancerous cells isolated from Sprague-Dawley rats in order to determineside effect level of FRI. In the experiments, cytotoxic (via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) tests), antioxidant (via totalantioxidant capacity (TAC) test), pro-oxidant (via total oxidative stress (TOS) test) and genotoxic (via 8-hydroxy-2′-deoxyguanosine (8-OH-dG) test) activities of different concentrations of FRI were tested. Asa result of the study, MTT assay revealed that FRI showed higher cytotoxic activity on U87MG cellscompared to PRCC cells (median inhibitory concentration (IC 50 ): 46.38 and 1271.77 mg/L, respectively).Based on U87MG cells, it was determined a significant positive correlation between LDH and TOSactivities. High positive correlation between TAC and cell viability on healthy PRCC cells exhibitedantioxidant capacity of FRI. Consequently, the results obtained from the present study proved thepotential of natural product with an antioxidant capacity as a source for anticancer compound againstGBM.
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