Abdullah KAHRAMAN, Ümran Akgün YILDIRIM, Melike BAKIR

SSR Markörlerinin Mercimek Genom Haritalamasında Kullanılması

Bu çalışmada, mercimek Lens culinaris Medik. bitkisinin genomuna ait bağlantı linkaj haritasına SSR Simple Sequence Repeats markörlerinin katılımı amaçlanmıştır. Genom haritası oluşturulmasında WA8649041 ve Precoz ebeveynlerin çaprazlanmasıyla elde edilen 93 adet rekombinant saf hat Rekombinant Inbred Lines, RILs populasyonu kullanılmıştır. Çalışmada 175 SSR primeri kullanılmış, bu primerlerden 25 adedi polimorfik, 109 adedi monomorfik, 41 adedi çalışmamıştır. Polimorfik olduğu tespit edilen SSR markörlerinden, 13 adedi haritalanmış, haritalanan markörler, genetik bağlantı haritasında belirli bir kromozom veya grupta toplanmayıp farklı bağlantı gruplarına dağılmıştır. Hali hazırda mevcut 123 markörden oluşan genom haritasına 57 AFLP, 48 RAPD, 16 ISSR ve 2 morfolojik markör , bu çalışmayla 13 SSR markör ilave edilerek genom haritasındaki toplam markör sayısı 136 olmuştur. Genom haritası 11 bağlantı linkaj grubundan oluşmuş, toplam harita uzunluğu 1311.2 cM ve iki markör arası ortalama uzunluk 9.64 cM olarak belirlenmiştir

Anahtar Kelimeler:

Mercimek, Moleküler Markör, SSR Basit Dizi Tekrarları, RIL Rekombinant

The objective of this study was to integrate the SSR Simple sequence Repeats markers in lentil Lens culinaris Medik. genetic linkage map. To construct the linkage map, an F6 derived Recombinant inbreed line RIL population with 93 lines developed from the cross of WA8649041 x Precoz was used. Linkage map was comprised of RAPD, ISSR, AFLP, ISSR and morphological markers. One hundred seventy five 175 SSR markers were used in the study. 25 SSR markers were polymorphic, while 109 SSR markers were monomorphic and 41 SSR markers did not work. SSR markers were distributed in different linkage groups rather than clustering in some linkage groups. Of the 21 SSR markers, 13 of them were mapped in lentil genome and the current genetic linkage map is comprised of 136 markers including 57 AFLP, 48 RAPD, 16 ISSR, 2 morphological and 13 SSR markers. Genetic linkage map is comprised of 11 linkage groups with 1311.2 cM in length and average genetic distance between two markers is 9.64 cM

Keywords:

Lentil Molecular Marker, SSR Simple Sequence Repeats, RIL Recombinant inbreed line, Linkage Map,

PDF

___

Kumar, S., Hamwieh, A., Manickavelu, A., Kumar, J., Sharma, T., R., And Baum, M. 2014. Advances İn Lentil Genomics.6, 111 Springer Science Business Media
Tanyolac, B., Ozatay, Ş., Kahraman, A., And Fred Muehlbauer. 2010. Linkage mapping of lentil (Lens culinaris L.) genome using recombinant inbred lines revealed by AFLP, ISSR, RAPD and some morphologic markers. Journal of Agricultural Biotechnology and Sustainable Development. 2(1 ); 001- 006
Muehlbauer, F., J., Cho S, Sarker A, Ford R 2006. Application of modern technologies in lentil breeding for biotic and abiotic stress resistance. Euphytica 147:149–165
Eujayl, I., Baum, M., Powell, W., Erskıne, W., And Pehu, E., 1998. A Genetic Linkage Map of Lentil (Lens sp.) Based on RAPD and AFLP Markers Using Recombinant Inbred Lines. Theor. Appl. Genet. 97: 83-89.
Hamwıeh, A., Udupa, S. M., Choumane, W., Sarker, A., Dreyer, F., Jung, C., And Baum, M., 2005. A Genetic Linkage Map of Lens sp. Based on Microsatellite and AFLP Markers and the Localization of Fusarium Vascular Wilt Resistance. Theor. Appl. Genet. 110: 669-677.
Rubeena, R., Taylor, W. J., Ford, P. And Ades, P. K., 2006. QTL Mapping of Resistance in Lentil (Lens culinaris ssp. culinaris) to Ascochyta Blight (Ascochyta lentis), Plant Breeding 125, 506-512.
Havey, M. H., And Muehlbauer, F. J., 1989. Linkages Between Restriction Fragment Length, Isozyme and Morphological Markers in Lentil. Theor Appl Genet 77:395–401.
Tahır, M., Erksıne, W., Hussaın, A., Baksh, A., Ellıs, R. H., Summerfıeld, R. J., And Roberts, E. H., 1993. Field Evaluation of a Model of Photothermal Flowering Responses in a World Lentil Collection. Theor. Appl. Genet 88: 423-428
Tullu, A., Tar'an, B., Warkentın, T., And Vandenberg, A., 2008.Construction of an Intraspecific Linkage Map and QTL Analysis for Earlinessand Plant Height in Lentil. Crop Sci 48:2254-2264.
Duran, Y., Fratını, R., Garcıa, P., And Vega, M. P., 2004. An Intersubspecific Genetic Map of Lens. Theor Appl Genet. 108: 1265-1273.
Rajesh, P. N., Whıte, D., Saha, , G., Chen, W., And Muehlbauer, F.,2008. Development and genetic analysis of SSR markers in lentil. Plant &Animal Genomes XVI Conference, January 12-16, 2008, Town & Country Convention Center, San Diego, CA
Hamwıeh, A., Udupa, S. M., Choumane, W., Sarker, A., Dreyer, F.,Jung, C., And Baum, M., 2005. A Genetic Linkage Map of Lens sp. Based on Microsatellite and AFLP Markers and the Localization of Fusarium Vascular Wilt Resistance. Theor. Appl. Genet. 110: 669-677.
Kosambı D. D., 1944, The Estimation of map distances from recombination values, Eugene 12: 172-17 Kahraman, A., Kusmenoğlu, I., Aydın, N., Aydoğan, A., Erksıne, W. And Muehlbauer, F. J., 2004. QTL Mapping of Winter Hardiness Genes in Lentil, Crop Science, 13-22.5
Phan, H., T., T., Ellwood, S., R., Hane, J., K., Ford, R., Materne, M., and Oliver, R., P. (2007) Extensive macrosynteny between Medicago truncatula and Lens culinaris ssp. Culinaris. Theoretical and Applied Genetics 114, 549–558.
Verma, P.,Goyal, R., Chahota, R., K., Sharma, T., R., Abdin, M., Z., Bhatia, S. 2015.Construction of a Genetic Linkage Map and Identification of QTLs for Seed Weight and Seed Size Traits in Lentil (Lens culinaris Medik.). Plos One. 5;10 (10).
Andeden, E., E., Baloch, F., S., Çakır, E., Toklu, F., Özkan, H. 2015. Development, characterization
and mapping of microsatellite markers for lentil (Lens culinarisMedik.). Plant Breeding 134: 589- 598.
Arumuganathan, K., Earle, E., D. 1991. Nuclear DNA content of some important plant .species. Plant Mol Biol Rep 9:208–218.
Ates, D., Aldemir, S., Alsaleh, A., Erdogmus, S., Nemli, S., Kahriman, A., Ozkan, H., Vandenberg, A., Tanyolac, B. 2018. A consensus linkage map of lentil based on DArT markers from three RIL mapping populations. PLoS ONE 13(1): e0191375.
Saha, G., C., Sarker, A., Chen, W., Vandemark, G., J., Muehlbauer, F., J., 2013. Inheritance and Linkage Map Positions of GenesConferring Agromorphological Traits in Lens culinaris Medik. Int J Agron 618926, 9 pages
Hendre, P., S., Aggarwal, R., K., 2007. DNA Markers: Development And Application For Genetic Improvement Of Coffee. In: Varshney RK, Tuberosa R. (ed) Genomics-Assisted Crop Improvement. Springer, Dordrecht, p 399–434.
Idrissi, O., Udupa, S.,M., Houasli, C., De Keyser, E., Van Damme, P., De Riek, J. 2015. Genetic diversity analysis of Moroccan lentil (Lens culinaris Medik.) landraces using simple sequence repeat and amplified fragment length polymorphisms reveals functional adaptation towards agro-environmental origins. Plant Breed 134, 322–332.
Toklu, F., Karaköy, T., Hakle, I., Bicer, T., Brandolini, A., Kilian, B., Ozkan, H. 2009. Genetic variation among lentil (Lens culinaris Medik.) landraces from Southeast Turkey. Plant Breed. 128:178–186.
Tsanakas, G., Mylona, P., Koura, K., Gleridou, A., Polidoros, A. 2018. Genetic diversity analysis of the Greek lentil (Lens culinaris) landrace ‘Eglouvis’ using morphological and molecular markers. Plant Genetic Resources: Characterization and Utilization 1-9.
Tanyolac, B., Ozatay, S., Kahraman, A., Muehlbauer, F. 2010. Linkage mapping of lentil (Lens culinaris L.) genome using recombinant inbred lines revealed by AFLP, ISSR, RAPD and some morphologic markers. J Agric Biotechnol Sustain Dev 2:001—006.