Aytül UÇAK KOÇ, Yusuf ATAKAN, Hüseyin KÜÇÜKER, Hüdai KÜÇÜKER

Denizli Kekik (Origanum onites) Balının Bazı Fiziko-Kimyasal Özellikleri

Bu çalışma ile, geniş kekik alanları ile gezginci arıcıların önemli konaklama merkezlerinden biri olan Denizli ili sınırları içinde konaklayan arıcıların sayısı ve konaklama yerleri belirlenmiş ve kekik balına ait bazı bilgiler elde edilmiştir. Özellikle Pamukkale ilçesine bağlı Gözler, Uzunpınar, Belenardıç ve Güzelpınar mahalleleri ile Güney ilçesine bağlı Yağcılar mahallelerinde kekik alanları yoğunlukta olup yaklaşık olarak 2021 yılında 24 bin arı kolonisi konaklamıştır. Bu alanlarda üretilen Denizli kekik balı hasadının yapıldığı Temmuz ayında toplanan, 2021 yılında 9 adet, 2022 yılında da 6 adet bal örneğinde bazı fizikokimyasal analizler yapılmıştır. Bal örneklerinde öncelikle polen analizi yapılmış ve kekik balı olduğu tespit edilmiştir. Daha sonra 15 bal örneğinde prolin, diastaz aktivitesi, nem, elektiriksel iletkenlik, pH, serbest asitlik, HMF, fruktoz, glukoz, toplam fenolik madde belirlenmiştir. Kekik balı örneklerinde ortalama prolin miktarı 2021 ve 2022 yılı sırasıyla; 663.5 ve 708.9 mg/kg, diastaz aktivitesi 10.9 ve 11.75; nem oranları %17.1 ve %17.3; EC 560 ve 540 µS cm-1 ; pH 3.8 ve 3.8; FA; 26.4 ve 28.8 meq.kg-1 ; HMF, 9.5 ve 12.4 mg/kg; F+G %69.6 ve %69; F/G 1.13 ve 1.10 olarak toplam fenolik madde 75.9 mg GAE/100g olarak tespit edilmiştir. Gelecekte, daha kapsamlı ve ileri tekniklerin uygulandığı analizlerin yapılması ile, Denizli kekik balını diğer ballardan ayırıcı özelliklerini ortaya koymak mümkün olabilecektir.

Anahtar Kelimeler:

bal arısı, koku, toplam fenolik içeriği, HMF, prolin, kekik

Some Physicochemical Characteristics of Denizli Thyme (Origanum onites) Honey

In this study, the number of beekeepers staying within the borders of Denizli province, which is one of the important accommodation centers of wandering beekeepers with its large thyme fields, and their accommodation places were determined and some information about thyme honey was obtained. Especially in Gözler, Uzunpınar, Belenardıç and Güzelpınar neighborhoods of Pamukkale district and Yağcılar neighborhoods of Güney district, thyme fields are dense and approximately 24 thousand beekeepers stayed in 2021. Some physicochemical analyzes were carried out, for example, 9 honeys in 2021 and 6 honeys in 2022, which were collected in July when the Denizli thyme honey produced in these areas was harvested. First, pollen analysis was made in honey samples, and it was determined that it was thyme honey. Then, proline, diastase activity (DA), moisture, electrical conductivity (EC), pH, free acidity (FA), HMF, fructose (F), glucose (G), total phenolic content (TPC) was determined in 15 honey samples. Average amount of proline in thyme honey samples in 2021 and 2022, respectively; 663.5 and 708.9 mg/kg, DA 10.9 and 11.75; humidity rates 17.1% and 17.3%; EC 560 and 540 µS cm-1 ; pH 3.8 and 3.8; FA; 26.4 and 28.8 meq.kg-1 ; HMF, 9.5 and 12.4 mg/kg; F+G 69.6% and 69%; F/G was determined as 1.13 and 1.10, and the TPC was determined as 75.9 mg GAE/100g. In the future, it will be possible to reveal the descriptive features of Denizli thyme honey from other honeys by conducting more comprehensive and advanced analyzes.

Keywords:

honey bee, smell, total phenolic content, HMF, proline, thyme,

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Andre J (1961) L’Alimentation et la cuisine a Rome. Paris.
Anonymous (2020) Turkish Food Codex (2020) Turkish Food Codex Communique on Honey (No: 2020/7). https:// www.resmigazete.gov.tr/eskiler/2020/04/20200422-13.htm.
Anonymous (2021) Denizli İl Tarım ve Orman Müdürlüğü, Denizli.
Akgün N, Çelik ÖF, Kelebekli L (2021) Physicochemical properties, total phenolic content, and antioxidant activity of chestnut, rhododendron, acacia and multifloral honey. Journal of Food Measurement and Characterization 15(4):3501-3508.
Balandier C (1993) Production et usages du miel dans l’antiquité gréco-romaine, Des hommes et des plantes, (Ed. M. C. Amouretti, G. Comet), Cahier d’histoire des techniques 2, 93-125.
Bouhlali EDT, Bammou M, Sellam K, El Midaoui A, Bourkhis B, Ennassir J, ... & Filali-Zegzouti Y (2019) Physicochemical properties of eleven monofloral honey samples produced in Morocco. Arab Journal of Basic and Applied Sciences 26(1): 476-487.
Can Z, Yıldız O, Sahin H, Akyuz Turumtay E, Silici S, Kolaylı S (2015) An investigation of Turkish honeys: their physico-chemical properties, antioxidant capacities and phenolic profiles. Food Chemistry 180, 133-141.
da Silva PM, Gauche C, Gonzaga LV, Costa ACO, Fett R (2016) Honey: Chemical composition, stability and authenticity. Food chemistry 196: 309-323.
Dobre I, Georgescu LA, Alexe P, Escuredo O, Seijo MC (2012) Rheological behavior of different honey types from Romania. Food Research International 49(1): 126-132.
Ecem Bayram N (2023) Nectar honey from Turkey: crystallization and physicochemical profile. European Food Research and Technology 249:1049–1057.
Güzel N, Bahçeci KS (2019) Çorum Yöresi Ballarının Fenolik Madde İçerikleri İle Renk Ve Antioksidan Kapasiteleri Arasındaki İlişki. Gıda, 44(6), 1148-1160.
Khan Z, Nanda V, Bhat MS, Khan A (2015) Kinetic studies of HMF formation and diastase activity in two different honeys of Kashmir. International Journal of Current Microbiology and Applied Sciences 4(4): 70-106.
Kıvrak Ş, Kıvrak I, Karababa E (2016) Characterization of Turkish honeys regarding of physicochemical properties, and their adulteration analysis. Food Science and Technology 37: 80-89.
Karacaoğlu M, Uçak Koç A (2007) Ege bölgesi arıcılığında kısıtlar ve fırsatlar. Ege Bölgesi Arıcılık Semineri, 15-16 Şubat 2007, İzmir, 25-32.
Karacaoğlu M, Gençer HV, Uçak Koç A, Kahya Y (2020) Arıcılık Sektöründe Mevcut Durum Kısıtlar Ve Fırsatlar. Türkiye Ziraat Mühendisliği IX. Teknik Kongresi 13-17 Ocak 2020, Ankara, 159-174.
Karatas S, Aktumsek A, Duru ME (2019) Investigation of Physicochemical Properties of Some Monofloral Honeys in South Western Anatolia. International Journal of Secondary Metabolite 6(3): 251-262.
Karataş Ş, Aktümsek A, Duru M E (2021) An investigation of the biological activity of monofloral honey produced in south-western Anatolia. International Journal of Secondary Metabolite 8(4): 300-311.
Laos K, Kirs E, Pall R, Martverk K (2011) The crystallization behaviour of Estonian honeys. Agronomy Research 9:427-432.
Lenger DS (2011) Antik Çağda Karia Bölgesinde Bal. Acta Turcica. Yıl III, Sayı 1/1, Ocak 2011 “Kültürümüzde Arıcılık”, Editörler: Emine Gürsoy Naskali, Hilal Oytun Altun s: 28-35.
Manzanares AB, Garcia H, Galdon BR, Rodriguez ER, Romero CD (2014) Physicochemical characteristics of minor monofloral honeys from Tenerife, Spain. Food Science & Technology 55: 572–578.
Meda A, Lamien CE, Romito M, Millogo J, Nacoulma OG (2005) Determination of the total phenolic, flavonoid and proline contents in Burkina Fasan honey, as well as their radical scavenging activity. Food chemistry 91(3): 571-577.
OAC (1997) Official methods of analysis (16th ed.). USA: Association of Official Analytical Chemist, Arlington, VA, Chapter 44, pp. 27-29, 1998., DIN 10758: Analysis of Honey - Determination of the content of saccharides fructose, glucose, saccharose, turanose and maltose-HPLC method.
Oddo LP, Piazza MG, Sabatini AG, Accorti M (2004a) Characterization of unifloral honeys. Apidologie 26: 453–485.
Oddo, L. P., Piro, R., Bruneau, É., Guyot-Declerck, C., Ivanov, T., Piskulová, J., ... & Ruoff, K. (2004b). Main European unifloral honeys: descriptive sheets. Apidologie, 35(Suppl. 1), S38-S81.
Özkök D, Silici S, Çam M (2010) Balda spesifikasyon ve kristalizasyonun (şekerlenme) balın kimyasal ve biyolojik özelliklerine etkisi. Erciyes Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi, FCD-2013-408 Nolu Proje raporu, 51 sayfa.
Özkök A, Koru Ö, Sorkun K (2016) Microbiological analysis and antibacterial effects of Turkish thyme honey. Bee World 93(4):98-101.
Rababah TM, Al-Omoush M, Brewer S, Alhamad M, Yang WD, Alrababah M, Al- Ghzawi AA, Al-U'Datt M, Ereifej K, Alsheyab F, Esoh R, Almajwal A (2014) Total phenol, antioxidant activity, flavonoids, anthocyanins and color of honey as affected by floral origin found in the Arid and Semiarid Mediterranean areas. J Food Process Preserv, 38(3): 1119-1128.
Serra Bonvehi J, Ventura Coll F (1995) Characterization of citrus honey (Citrus spp.) produced in Spain. Journal of Agricultural and Food Chemistry 43(8): 2053-2057.
Serrano S, Espejo R, Villarejo M, Jodral ML (2007) Diastase and invertase activities in Andalusian honeys. International Journal Of Food Science & Technology 42(1):76-79.
Singleton VL, Rossi JA (1965) Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American journal of Enology and Viticulture 16(3):144-158.
Terrab A, Diez MJ, Heredia FJ (2002) Characterisation of Moroccan unifloral honeys by their physicochemical characteristics. Food Chemistry 79(3): 373-379.
Terrab A, Recamales AF, Hernanz D, Heredia FJ (2004) Characterisation of Spanish thyme honeys by their physicochemical characteristics and mineral contents. Food Chemistry 88(4): 537-542.
TUIK, (2020). Türkiye İstatistik Kurumu, www.tuik.gov.tr
Venir E, Spaziani M, Maltini E (2010) Crystallization in “Tarassaco” Italian honey studied by DSC. Food Chemistry 122(2): 410-415.