Nur DOKUZEYLÜL GÜNGÖR, Tuğba GÜRBÜZ, Aynur ERŞAHİN

The effects of transfer day in intracytoplasmic sperm injection (ICSI) pregnancies on first trimester screening test results

Objects To compare first trimester screning serum markers free β-human chorionic gonodotropin (free β-hCG and pregnancy associated plasma protein A (PAPP-A) of blastocyst versus cleavage stage embryo transfers after assisted reproductive technologies (ART) . Methods Retrospective analysis of 123 women who conceived with ART undergone first-trimester screening between January 2017 and December 2017 at Bahçeşehir University Göztepe Medical Park Hospital IVF Unit. The age, gestational week, ultrasound markers and biochemistry markers (PAPP- A,free β-hCG) were collected. Results were considered significant when the p value was less than 0.05. Statistical Package For Social Sciences (SPSS) version 17.0 for Windows was used for statistical analysis. Results PAPP-A levels were significantly lower in day-3 (cleavage stage embryos) transfer intracytoplasmic sperm injection (ICSI) pregnancies compared to day-5 (blastocyst stage embryos) transfer ICSI pregnancies (p<0.05). Free β-hCG levels weren’t significantly differ in two groups (p=0.371). NT was unaffected by the day of transfer (p=0.21). Also CRL wasn’t affected by the day of transfer (p=0.693). No differences appeared between day-3 and day-5 embryo transfer with respect to maternal age ,(p=0.616) weight (p=0.693) and gestational age (p=0.742) at sampling. Conclusion Today, the data on the effect of ART on the components of first trimester combined screening for chromosomal analysis are still controversial. Day-3 ICSI pregnancies had significantly lower PAPP-A levels supporting the need to appropriately adjust the combined first trimester screening (cFTS) risk algoritm. These results may be due to alterations in the placentation of ART pregnancies.

Anahtar Kelimeler:

Assisted reproduction techniques, blastocyst, cleavage, first trimester screening

The effects of transfer day in intracytoplasmic sperm injection (ICSI) pregnancies on first trimester screening test results

Objects: To compare first trimesterscrening (FTS) serum markers free β-human chorionic gonodotropin (free β-hCG and pregnancy associated plasma protein A (PAPP-A) of blastocyst versus cleavage stage embryo transfers after assisted reproductive technologies (ART).Methods: Retrospective examinations of 123 women who conceived with ART were subjected to FTS from January 2017 to December 2017 at Bahçeşehir University Göztepe Medical Park Hospital In Vitro Fertilization (IVF) Unit.The age,gestational week,ultrasound (US) markers and biochemistry markers(PAPP-A,free β-hCG) were collected.Outcomes were regarded as meaningful when the p < 0.05. Statistical Package For Social Sciences (SPSS) v.17.0 for Windows was preferred to make statistical analysis.Results: PAPP-A amounts were remarkably low in day-3 (cleavage stage embryos) transfer intracytoplasmic sperm injection (ICSI) pregnancies compared to day-5 (blastocyst stage embryos) transfer ICSI pregnancies.Free β-hCG amounts weren’t significantly different in two groups (p=0.371). NT was unaffected by the transferenceday (p=0.21). Also CRL wasunaffected by the transferenceday (p=0.693). No differences appeared between 3rd and 5th day embryo transference according to maternal age (p=0.616), weight (p=0.693) and gestational age (p=0.742) at sampling. Conclusion: Today, the datas about the effects of ART over the ingredients of combined FTS for chromosomal analysis are contentious yet.Day-3 ICSI pregnancies had meaningfully low PAPP-A amounts which support the requirement to properly set the combined FTS risks algoritm. These outcomes are likely to be because of changings in the placenta of ART pregnancies.

Keywords:

Assisted reproduction techniques, blastocyst, cleavage, first trimester screening,

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