Mousa TAVASSOLI, Bahram DALIR-NAGHADEH, Sima VALIPOUR, Mina MAGHSOUDLO

Prevalence of Gastrointestinal Parasites in Water Buffalo (Bubalus bubalis) Calves Raised with Cattle in Smallholder Farming System in the Northwest of Iran

The objective of this study was to investigate the prevalence of gastrointestinal parasitic infection in buffalo calves in Northwest of Iran. Fecal samples of 317 buffalo calves under 9 months of age (155 male and 162 female) were collected from villages around Urmia, Northwest of Iran. Centrifugal flotation technique using saturated zinc sulphate solution and sedimentation methods was used to detect protozoa and helminthes infections. The modified Ziehl-Neelsen technique was used for determining Cryptosporidium spp., infection. Eight Eimeria species oocysts were identified in 112 (35.3%) out of the 317 buffalo calves. Eimeria zuernii had the highest prevalence (23.3 %) followed by E. bovis (15.1%), E. ellipsoidalis (7.3%), E. bareillyi (2.5%), E. bukidnonensis (2.2%), E. ovoidalis (1.3%), E. cylindrical (0.9%), E. auburnensis (0.6%). No cysts of Giardia spp. were detected. The oocysts of Cryptosporidium spp. were detected in 8 (2.5%) buffalo calves. Twenty-three buffalo calves (7.3%) were positive for Toxocara vitulorum eggs. No clinical finding was observed in infected buffalo. In conclusion, Eimeria spp., Cryptosporidium spp and T. vitulorum are relatively common in buffalo calves and buffalo should be considered as a potential source of infection to other animal species.

İran'ın Kuzeybatı Bölgesindeki Küçük Çaplı Yetiştiricilik Sisteminde Sığırlar ile Birlikte Yetiştirilen Manda (Bubalus Bubalis) Yavrularında Gastrointesinal Parazitlerin Prevalansı

Bu çalışmanın amacı, İran’ın kuzeybatı bölgesindeki manda yavrularında gastrointestinal paraziter enfeksiyon prevalansını araştırmaktır. 9 aylıktan küçük 317 manda yavrusuna (155 erkek ve 162 dişi) ait dışkı örnekleri, İran’ın kuzeybatısında bulunan Urmia bölgesi etrafındaki köylerden toplanmıştır. Protozoon ve helmint enfeksiyonlarını belirlemek için; doymuş çinko sülfat çözeltisinden yararlanılarak santrifüj flotasyon tekniği ve sedimentasyon yöntemleri kullanılmıştır. Cryptosporidium spp. enfeksiyonunu belirlemek için modifiye Ziehl-Neelsen tekniği uygulanmıştır. 317 manda yavrusundan 112’sinde (%35,3) 8 Eimeria türü ookist tespit edilmiştir. Eimeria zuernii en yüksek prevalansa (%23,3) sahipken; bunu E. bovis (%15,1), E. ellipsoidalis (%7,3), E. bareillyi (%2,5), E. bukidnonensis (%2,2), E. ovoidalis (%1,3), E. cylindrical (%0,9), E. auburnensis (%0,6) takip etmiştir. Hiçbir Giardia spp. kisti tespit edilmemiştir. Cryptosporidium spp. ookistleri 8 (%2,5) manda yavrusunda tespit edilmiştir. 23 manda yavrusu (%7,3) Toxocara vitulorum yumurtaları yönünden pozitif bulunmuştur. Enfekte mandalarda hiçbir klinik bulgu gözlenmemiştir. Sonuç olarak, Eimeria spp., Cryptosporidium spp. ve T. vitulorum, manda yavrularında kısmen yaygındır ve mandalar, diğer hayvan türleri için potansiyel bir enfeksiyon kaynağı olarak düşünülmelidir.

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Appelbee, A.J., Thompson, R.C., Olson, M.E., 2005. Giardia and Cryptosporidium in mammalian wildlife-current status and future needs. Trends Parasitology 21, 370-376. [CrossRef]

Azami, M., 2007. Prevalence of Cryptosporidium infection in cattle in Isfahan, Iran. Journal of Eukaryot Microbiology 54(1), 100-102. [CrossRef]

Baruah, P.K., Singh, R.P., Bali, M.K., 1979. Studies on some of the biochemical and haematological changes in buffalo calves infected with Neoascaris vitulorum. Haryana Veterinary 18, 107-110.

Borgsteede, F.H.M., Holzhauer, M., Herder, F.L., Veldhuis-Wolterbeek, E.G., Hegeman, C., 2012. Toxocara vitulorum in suckling calves in the Netherlands. Research in Veterinary Science 92, 254- 256. [CrossRef]

Caccio, S.M., Rinaldi, L., Cringoli, G., Condoleo, R., Pozio, E., 2007. Molecular identification of Cryptosporidium parvum and Giardia duodenalis in the Italian water buffalo (Bubalus bubalis). Veterinary Parasitology 150(1-2), 146-149. [CrossRef]

Chibunda, R.T., Muhairwa, A.P., Kambarage, D.M., Mtambo, M.M.A., Kusiluka, L.J.M., Kazwala, R.R., 1996. Eimeriosis in dairy cattle farms in Morogoro municipality of Tanzania. Preventive Veterinary Medicine 31, 191-197. [CrossRef]

Dalir-Naghadeh, B., Tavassoli, M., Hatefnia, A.Z., 2008. Study of epidemiologic measures of association between Giardia Sp. infection with occurrence of diarrheain calves. Journal of Veterinary Research 62(6), 363-366.

Davila, G., Max, I., Ellis, C., 2010. Toxocara vitulorum in beef calves in North Central Florida, Veterinary Parasitology 168(3-4), 261- 263. [CrossRef]

Eslami, A., Hosseini, H, 1989. Analysis of parasitic infections in animal husbandry around Tehran, Journal of Veterinary Research 44(3), 35-40.

Fayer, R., 2010. Taxonomy and species delimitation in Cryptosporidium. Experimental Parasitology 124, 90-97. [CrossRef]

Fotouhi Ardakani, R., 2008. Epidemiology of Cryptosporidium Infection of Cattle in Kerman/Iran and Molecular Genotyping of some Isolates, Journal of Kerman Univesity Medical Science 15(4), 313-320.

Gundrans, R.S., More, S.J., 1999. Health and growth of water-buffalo calves in Nueva Ecija, the Philippines. Preventive Veterinary Medicine 40, 87-100. [CrossRef]

Gupta, P.P., Singh, B., Mandal, P.C., Gill, B.S., Grewal, G.S., 1978. A Postmortem study of mortality pattern in adult buffaloes in Punjab. Indian Journal of Animal Science 48, 69.

Huetink, R.E.C., Giessen, J.W.B., Noordhuizen, J.P.T.M., Ploeger, H.W., 2001. Epidemiology of Cryptosporidium spp. and Giardia duodenalis on a dairy farm. Veterinary Parasitology 102, 53-67. [CrossRef]

Lau, H.D., 1987. Principal disease of un-weaned buffalo calves in Parastate, Brazil. (Portuguese). Boletim de Pesquisa, Centro de Pesquisa Agropecuaria do Tropico Umido, Embrapa, Brazil 83, 12.

Levine, N.D., 1985. Veterinary Protozology. Iowa State University Press, Ames, p. 414.

Matjila, P.T., Penzhorn, B.L. 2002. Occurrence and diversity of bovine coccidian at three localities in South Africa. Veterinary Parasitology 104, 93-102. [CrossRef]

Mtambo, M.M., Sebatwale, J.B., Kambarage, D.M., Muhairwa, A.P., Maeda, G.E., Kusiluka, L.J., Kazwala, R.R. 1997. Prevalence of Cryptosporidium spp. Oocysts in cattle and wild life in Morogoro region, Tanzania. Preventive Veterinary Medicine 31(3-4), 185-900. [CrossRef]

Munyua, W.K., Ngotho, J.W., 1989. Prevalence of Eimeria species in cattle in Kenya. Veterinary Parasitology 35, 163-168. [CrossRef]

Nalbantoglu, S., Sari, B., Cicek, H., Karaer, Z., 2007. Prevalence of Coccidian Species in the Water Buffalo (Bubalus Bubalis) in the Province of Afyon, Turkey. Acta Veterinary Brno 77, 111-116. [CrossRef]

O’Handley, R.M., 1999. Duration of naturally acquired giardiasis and cryptosporidiosis in dairy calves and their association with diarrhea. Journal of American Veterinary Medicine Association 214, 391-396.

Patnaik, M.M., Pande, B.P., 1965. Some observations on the endogenous stages of species of Eimeria and related lesions in naturally infected buffalo calves (Bubalus bubalis). Indian Journal of Veterinary Science 35, 33-46.

Penzhorn, B.L., 2000. Coccidian oocyst and nematode egg counts of free-ranging African buffalo (Synceruscaffer) in the Kruger National Park, South Africa. Journal of South African Veterinary Association 71, 106-108. [CrossRef]

Radfar, M.H., Molaei, M.M., Baghbannejad, A., 2006. Prevalence of Cryptosporidium spp. Oocyts in dairy calves in kerman, Southwestern Iran. Iranian Journal of Veterinay Research 7, 81-84.

Radostits, O.M., Gay, C.C., Hinchcliff, K.W., Constable, P.D., 2007. Veterinary Medicine: A textbook of the diseases of cattle, horses, sheep, pigs, and goats. 10th ed, USA, Vol 1-2, pp. 260-268.

Ribeiro, M.G., Langoni, H., Jerez, J.A., Leite, D., Ferreira, F., Gennari, S.M. 2000. Identification of enteropathogens from buffalo calves with and without diarrhoea in the Ribeira Valley, State of São Paulo, - Brazil. Brazilian Journal of Veterinary Research and Animal Science 37, 159-165. [CrossRef]

Rinaldi, L., Musella, V., Condoleo, R., Saralli, G., Veneziano, V., Bruni, G., Condoleo, R.U., Cringoli, G., 2007. Giardia and Cryptosporidium in water buffaloes (Bubalus bubalis). Parasitology Research 100(5), 1113-1118. [CrossRef]

Samizadeh-Yazd, A., Karimi, A., Ahmadi, M., 1981. Neoascariasis in buffalo calves of North-West of Iran. Journal of Veterinay Faculty University of Tehran 37, 43-49.

Santin, M., Trout, J.M., Xiao, L., Zhou, L., Greiner, E., Fayer, R., 2004. Prevalence and age-related variation of Cryptosporidium species and genotypes in dairy calves. Veterinary Parasitology 122, 103-117. [CrossRef]

Sayin, F., 1968, The sporulated oocysts of Eimeria ankarensisn. sp. and of other species of Eimeria of buffalo in Turkey and transmission of four species of Eimeria from buffalo to cow calves. Ankara University Veterinary Fakulty Dergici 15, 282-300.

Sayin, F., 1973. The presence of Eimeria bareillyi (Gill, Chhabra and Lal, 1963) in buffalo in Turkey. Ankara University Veterinary Fakulty Dergici 20, 38-42.

Selim, M.K., Tawfik, M.A.A., 1966. Incidence of Ascaris vitulotum in the Egyptian buffaloes during the late autumn and early winter in the United Arab republic. Indian Veterinary Journal 43, 965-968.

Shastri, U.V., Krishnamurthi, R., 1975. A note on pathological lesions in clinical bubaline coccidiosis due to Eimeria bareillyi. Indian Journal of Animal Science 45(1), 46-47.

Srikitjakaran, L., Lohr, D.R., Leidl, K., Horchner, F., 1987. Metaphylactic deworming program for buffalo calves in North- East Thailand. Tropical Medicine Parasitology 38, 191-193.

Soulsby, E.L.J., 1982. Helminths, Artropods and Protozoa of Domesticated Animals. 7th ed, BailliereTindall, London, p. 809.

Tavassoli, M., 1999. Studies on Toxocara vitulorum infection f buffalo calves in Urmia, Iran. Journal Veterinary Parasitology 13(2), 159-160.

Thienpont, D., Dekeyser, H., 1981. Toxocariasis of cattle in Belgium: Epidemiology and control of nematodiasis of cattle. Current Tropical Veterinary Medicine and Animal Science 9, 581-596. [CrossRef]

Thompson, R.C., Monis, P.T., 2004. Variation in Giardia: implications for taxonomy and epidemiology. Advanced Parasitology 58, 69- 137. [CrossRef]