Effect of Solvents on Phytoconstituents and Antimicrobial Activities of Ocimum gratissimum and Eugenia caryophyllata Extracts on Listeria monocytogenes

This study examined the effect of different solvents on active ingredients and antimicrobial performance of fresh leaves of Ocimum gratissimum and dried buds of Eugenia caryophyllata. Plant parts were extracted in a soxhlet apparatus using dietyl-ether, ethyl acetate, and ethanol. Phytoconstituents of the plants was determined using gas chromatography-mass spectrometer (GC-MS). The extract with high eugenol concentrations was prepared in seven treatments (160, 80, 40, 20, 10, 5, and 0 mg/mL) and tested against Listeria monocytogenes using agar well diffusion methods in triplicates. Minimum inhibitory concentration (MIC) and zone of inhibition were determined using standard technique. The data was subjected to descriptive statistics and one-way analysis of variance (ANOVA) at α0.05. The results showed that the phytochemical concentration of the extract varied significantly (p

Solventlerin fitobileşenler ve Ocimum gratissimum ve Eugenia caryophyllata Ekstraktlarının Listeria monocytogenes’e Karşı Antimikrobial aktiviteleri Üzerine Etkileri

Bu çalışmada farklı solventlerin aktif maddeler ile Ocimum gratissimum’un taze yaprakları ve Eugenia caryophyllata’nın kuru tomurcuklarının antimikrobial performansları üzerindeki etkileri araştırıldı. Bitki parçacıkları soxhlet cihazı içerisinde dietil eter, etil asetat ve etanol kullanılarak ekstrakte edildi. Bitkilerin fito-bileşenleri, gaz kromatografisi- kütle spektrometresi (GC-MS) kullanılarak saptandı. Yüksek öjenol konsantrasyonlu ekstrakt 7 işlemde hazırlandı (160, 80, 40, 20, 10, 5, and 0 mg/ mL) ve Listeria monocytogenes’e karşı agar kuyucuk difüzyon metodu ile 3 kez tekrar edilerek test edildi. Minimum inhibitör konsantrasyonu (MIC) ve inhibisyon sahası standart teknik kullanılarak tespit edildi. Veriler istatistiki olarak hesaplandı ve α0.05 düzeyinde tek yönlü varyans analizi (ANOVA) yapıldı. Sonuçlara göre ekstraktın fito-kimyasal konsantrasyonu solventler ile ilişkili olarak anlamlı ölçüde (p


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