Süreyya CEYLAN, Gülay YEGİNOĞLU, Melda YARDIMOĞLU, Sibel KÖKTÜRK

İnsan Mide Mukozasının Erken ve Geç Fetal Gelişim Surecindeki Histokimyasal Özellikler

Bu çalışmada 7 fetal mide antrum biyopsi örneklerinde mide mukoza bezlerinin histoşimik özellikleri araştırıldı. PAS (Perriyodik Asid Schiff), AB (Alcian Blue) pH 2.5 ve HID (Yükseltgenmiş Demir Diamin Aktivitesi) histokimyasal reaksiyonları ile mide mukoza bezlerinde mukosubstansların gelişim sürecindeki diferensiasyon ve matürasyon ile ilişkisi araştırıldı. PAS (pH 7) nötr mukopolisakkaritlerin, HID ve AB (pH 2.5) asit mukopolisakkaritlerin bez epitel hücrelerinde ışık mikroskobunda gösterilebilmesi için kullanıldı. CEA (Carcinoembryonic Antigen) proliferasyon bildiren bir antigen olduğu ve bunun fetal gelişimdeki araştırma tekniği olarak değeri tartışıldı. Embriyonal proliferasyonun bir kriteri olması nedeniyle CEA fetal matürasyon sürecinde azalmaktadır.

Anahtar Kelimeler:

Fetal Mide Antrumu, Karsinoembryonik Antijen, Periyodik Asid Schiff, Alcian Blue, Yükseltgenmiş Demir Diamin Aktivitesi.

Histochemical Characteristics of Early and Late Fetal Period of Human Stomach Mucosa

In this study, histochemical characteristics of mucous glands of the seven fetal gastric antrum are investigated. The relation between the presence of gastric acid mucosubstances by PAS, AB (pH 2.5), I-/ ID staining techniques and differentiation and maturation is searched. PAS, HID and AB (p H 2.5) staining techniques as indicator on the neutral and acid mucopolysaccarides demonstration of gland epithelial are used. CEA is known as proliferation antigen and value of CEA as a research techniques is discussed in the fetal maturation. CEA as criterion of embryonic proliferation decreases on the fetal maturation.

Keywords:

AB (pH 2.5), CEA, HID, PAS, Fetal Stomach,

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