Farklı Zenginleştirici Emülsiyonların Rotifer (Brachionus plicatilis) Yığın Kültüründe Kullanımı

Bu çalışmada, rotifer Brachionus plicatilis kültüründe 2 formüle emülsiyon (Emülsiyon-I ve Emülsiyon-II) ve 3 farklı ticari ürün (Olio w-3, Red Pepper ve (n-3) Top Rich) kullanımının popülasyon artışı üzerine etkisi araştırılmıştır. Rotiferler için oluşturulan deneme grupları sırasıyla; DHA-Gold (Kontrol), DHA-Gold + Emülsiyon-I (1. Grup), DHA-Gold + Emülsiyon-II (2. Grup), DHA-Gold + Olio w-3 (3. Grup), DHA-Gold + Red Pepper (4. Grup), DHA-Gold + (n-3) Top Rich (5. Grup) olarak belirlenmiştir. Farklı besleme rejimine göre rotiferlerin populasyon artışının belirlenmesi için 1 L kültür hacminde yığın kültür, başlangıç yoğunluğu 100 birey/ml olacak şekilde 7 gün süre ile gerçekleştirilmiş ve günlük olarak birey sayımları yapılmıştır. Deneme sonunda rotiferlerde en yüksek birey sayısı, büyüme hızı ve fekondite oranı DHA-Gold + Emülsiyon-II ile zenginleştirilen grupta sırasıyla 1250 ± 11,54 birey/ml, 0,37 ± 0,00 bölünme/gün ve 0,38 ± 0,01 yumurta/birey olarak tespit edilmiş olup, söz konusu değerler kontrol grubundan istatistiksel olarak önemli derecede farlı bulunmuştur (P<0.05). Emülsiyon ilavesinin rotiferin popülayon artışı, büyüme hızı ve fekondite oranını olumlu yönde etkilediği belirlenmiştir.

Anahtar Kelimeler:

Rotifer, Brachionus plicatilis, popülasyon artışı, emülsiyon

Use of Different Enrichment Emulsions in Rotifer (Brachionus plicatilis) Batch Culture

In this study, the effect of using 2 formulated emulsions (Emulsion-I and Emulsion-II) and 3 different commercial products (Olio w-3, Red Pepper, and (n-3) Top Rich) on population growth in rotifer Brachionus plicatilis culture was investigated. Experimental groups created for rotifers, respectively; DHA-Gold (Control), DHA-Gold + Emulsion-I (1st Group), DHA-Gold + Emulsion-II (2nd Group), DHA-Gold + Olio w-3 (3rd Group), DHA-Gold + Red Pepper (4th Group), DHA-Gold + (n-3) Top Rich (5th Group). To determine the population growth of rotifers according to different feeding regimes, bulk culture in 1 L culture volume was carried out with an initial density of 100 individuals/ml for 7 days and individual counts were made daily. At the end of the experiment, the highest individual number, growth rate, and fecundity ratio in the rotifers were determined as 1250 ± 11.54 individuals/ml, 0.37 ± 0.00 divisions/day, and 0.38 ± 0.01 eggs/individual in the group enriched with DHA-Gold + Emulsion-II, respectively, and these values were found to be statistically significantly different from the control group (P<0.05). It was determined that the addition of emulsion positively affected the population increase, growth rate, and fecundity rate of rotifer.

Keywords:

Rotifer, Brachionus plicatilis, population growth, emulsion,

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