Uğur MERCAN, Yonca Betil KABAK, Akif TÜRER, Osman KELAHMETOĞLU, Deniz Gokce MERAL

The effects of a single high-dose injection of vitamin-D on healing of condylar fractures in osteoporotic rats

Aim: In this study we have tried to show the effects of a single high-dose ınjection of vitamin d on healing of condylar fractures in osteoporotic rats. Materials and method: This study included sixteen female wistar rats. All rats were overiectomized. Osteoporotic rats were randomly divided into two groups of eight animals/ group. Group D animals received a single high dose of vitamin D (50.000 IU/kg) intraperitoneally and group C (control) animals received saline (0.05 mL) intraperitoneally. All injections were given immediately after condylar fracture. All rats were euthanized after 28 days, examined histopathological and immunohistochemical. Results: Histopathological and immunohistochemical evaluations were done. Histopathological scores between group D and C were found statistically significant. TGFβ1 and osteopentin levels in group D were found higher than group C. Conclusion: We have determined that a single high-dose injection of vitamin D given immediately after surgery increased TGFβ and osteopontin levels and enhanced new bone formation at condylar fractures of osteoporotic rats.

Osteoporotik sıçanlarda tek yüksek doz D-vitamini enjeksiyonunun kondil kırığı iyileşmesine etkisi

Amaç: Bu çalışmada, osteoporotik sıçanlarda kondiler kırıkların iyileşmesinde tek yüksek doz verilen D vitamininin etkilerini göstermeye çalıştık. Gereç ve Yöntem: Bu çalışmada 16 dişi wistar sıçanı kullanıldı. Tüm sıçanlara overiektomi uygulandı. Osteoporotik sıçanlar rastgele her grupta sekiz hayvan olacak şekilde iki gruba ayrıldı. Grup D hayvanlarına intraperitoneal olarak tek doz D vitamini (50,000 IU / kg) verildi ve grup C (kontrol) hayvanlara intraperitoneal olarak serum fizyolojik (0,05 mL) verildi. Tüm enjeksiyonlar, kondiler kırıktan hemen sonra yapıldı. Bütün sıçanlar 28 gün sonra sakrifiye edildi, Histopatolojik ve immünohistokimyasal olarak incelendi. Bulgular: Histopatolojik ve immünohistokimyasal değerlendirmeler yapıldı. D ve C grubu arasındaki histopatolojik veriler istatistiksel olarak anlamlı bulundu. Grup D' de TGFβ1 ve osteopentin düzeyleri C grubundan daha yüksek bulundu. Sonuç: Cerrahi müdahaleden hemen sonra verilen tek yüksek doz D vitamini enjeksiyonunun, osteoporotik sıçanların kondiler kırıklarında TGFβ ve osteopontin seviyelerini ve yeni kemik oluşumunu arttırdığını tespit ettik.

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