Civa Klorid ve Kurşun Nitrat'ın Erkek Ratlarda Kardiyotoksik Etkisi

Amaç: Kurşun nitrat ve civa klorid insan ve hayvan dokularında çeşitli değişikliklere yol açan çevresel ve endüstriyel kirleticilerdir. Bu çalışmada, kurşun nitrat ve civa klorid'in erkek sıçanlardaki kardiyotoksik etkisi araştırılmıştır. Materyal ve metod: Kurşun nitrat (LN, 45 mg/kg vücut ağırlığı) ve civa klorid (MC, 0.02 mg/kg vücut ağırlığı) ve bunların kombinasyonları 4 hafta boyunca oral yoldan hayvanlara uygulanmıştır. Bu çalışmada 4 deney grubu bulunmaktadır: kontrol, kurşun nitrat, civa klorid, kurşun nitrat ve civa klorid. Deney bitiminde kalp dokularındaki malondialdehit seviyesi (MDA), antioksidan enzim aktiviteleri (süperoksit dismutaz (SOD), katalaz (CAT), glutatyon peroksidaz (GPx), gluıtatyon-S- transferaz (GST) değerlendirme yapılmıştır. Bulgular: Kontrol grubu ile karşılaştırıldığında, kurşun nitrat, civa klorid ve kurşun nitrat+civa klorid uygulaması SOD, CAT, GPx ve GST aktivitelerinde artışa yol açmıştır. Aynı zamanda MDA seviyesi de artmıştır. Işık mikroskobu incelemelerinde uygulama gruplarında kalp dokularında ciddi histopatolojik değişimler gözlenmiştir. Sonuç: Kurşun nitrat ve civa kloridin birlikte uygulanmasının tek başlarına uygulanmalarına göre daha toksik etkili olduğu tespit edilmiştir.

Anahtar Kelimeler:

Kurşun, Civa, Oksidatif Stres, Patoloji, Kalp

Effects of Mercury Chloride And Lead Nitrate Induced Cardiotoxicity in Male Rats

Background: Lead nitrate and mercury chloride are widespread environmental and industrial pollutant, which induces severe alterations in the body tissues of both humans and animals. In the present study, the effects of lead and mercury induced cardiotoxicity was studied in Wistar rats. Methods: Lead nitrate (LN, 45 mg/kg bw/day) and mercury chloride (MC, 0.02 mg/kg bw/day) and their combination were administered orally for four weeks. Four groups of rats were used in the study: control, LN, MC and LN plus MC groups. Malondialdehyde (MDA) level, antioxidant enzyme activities [superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione-S-transferase (GST)] and histopathological changes in heart tissue were evaluated. Results: Results showed that LN and MC exposure and also their combination resulted in an increase in the SOD, CAT, GPx, GST activities and also MDA levels, with respect to the control group. Light microscopic investigations revealed that mercury chloride and lead nitrate and also co-treatment of them induced histopathological changes in heart tissue. Conclusion: As a result, combination of LN plus MC was more harmful than use them alone.

Keywords:

Lead, Mercury, Oxidative Stress, Pathology, Heart,

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