Aykut PELİT, Mustafa EMRE, Ayşe DEMİRKAZIK, İsmail GÜNAY

Streptozotosin ile Deneysel Diyabet Oluşturulan Sıçanların Soleus Kaslarının Elektrofizyolojik Parametrelerine Elektromanyetik Alanın Etkileri

Amaç: Streptozotosin (STZ) ile deneysel diyabet oluşturulan sıçanlar elektromanyetik alana maruz bırakıldıktan sonra soleus kas preparatlarından dinlenim zar potansiyeli ve kas aksiyon potansiyelinin ölçülerek incelenmesi. Metod: Bu çalışmada 40 adet Wistar türü erkek sıçan kullanıldı. Sıçanlar dört gruba bölündü; Grup I (Kontrol), Grup II (Manyetik alan kontrolü), Grup III (Diyabetik), Grup IV (diyabet uygulanmış manyetik alan grubu). Diyabet tek doz halinde (45 mg/kg ) ketamin ve ksilazin kombinasyon anestezisi altında citrat tamponu içerisine karıştırılmış STZ olarak jügüler venden verildi. Konvensiyonel mikroeletrod tekniği ile dinlenim zar potansiyeli, aksiyon potansiyeli, depolarizasyon ve repolarizasyon süresi, kas aksiyon potansiyeli integrali kayıtlandı. Sonuçlar: Dinlenim zar potansiyeli, Grup I, III ve IV arasında anlamlı bir azalma bulunmuştur (p

Anahtar Kelimeler:

Soleus kası, streptozotosin, Dinlenim zar potansiyeli, Aksiyon Potansiyeli, Manyetik Alan.

Effects of Magnetic Field on the Electrophysiological Parameters of Soleus Muscle in Streptozotocin-Induced Diabetic Rats

Purpose: To investigate the effects of magnetic field (MF) on the resting membrane potential and action potential parameters of the Soleus muscle preparations in streptozotocin (STZ) induced diabetic rats. Methods: Forty male wistar rats were used in this study. The rats were divided into four groups; Group I (control), Group II (control with magnetic field), group III (diabetic), group IV (diabetic with magnetic field). The diabetes was induced by a single intravenous injection of STZ (45 mg/kg) in citrate buffer to the jugular vein under the ketamine and xylazine anesthetic combination. Resting membrane potential (RMP), muscle action potential (MAP), depolarization and half-repolarization time, MAP integral, depolarization and repolarization rates were recorded by means of the conventional microelectrode technique. Results: When group III and IV compared to group I, a significant decrease was found in the RMP (p

Keywords:

Soleus muscle, streptozotocin, resting membrane potential, action potential, magnetic field,

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Paulus SF, Grossie J. Skeletal muscle in alloxan diabetes a comparison of isometric contractions in fast and slow muscle. Diabetes. 1983; 32:1039-53..
Bassett CA. Beneficial effects of EMFs. Journal of Cellular Biochemistry. 1993; 51:387-93.
McLeod KJ, Rubin CT. The effect of low-frequency electrical fields on osteogenesis. Journal of Bone and Joint Surgery. 1992; 74:920-9.
Sisken BF, Kanje M, Lundborg G, Herbst E, Kurtz W. Stimulation of rat sciatic nerve regeneration with pulsed electromagnetic fields. Brain Research.1989; 485:309-16.
McArdle JJ, Michelson L, D’Alanzo AJ. Action Potentials in Fast- and Slow-Twitch Mammalian Muscles during Reinnervation and Development. J. Gen Physiol. 1980; 75:655-72.
Hida W, Shindoh C, Satoh J, Sagara M, Kikuchi Y, Toyota T, Shirato K. N-acetylcysteine inhibits loss of diaphragm function in streptozotocin-treated rats. Am. J. Crit. Care Med. 1996; 153:1875-9.
McGuire M, MacDermott M. The influence of streptozotocin-induced Diabetes and the antihyperglycaemic agent metformin on the contractile characteristics and the membrane potential of the rat diaphragm. Exp. Physiol. 1998; 83:481-7.
McGuire M, Dumbleton M, MacDermott M, Bradford A. Contractile and electrical properties of sternohyoid muscle in streptozocin diabetic rats. Clin. Exp. Pharm. Physiol. 2001; 28:184-7.
Grossie J. Contractile and electrical characteristics of extensor muscle from alloxan-diabetic rats. An in vitro study. Diabetes. 1982; 31:194-202.
Kanje M, Rusova A, Sisken B, Lundborg G. Pretreatment of Rats with Pulsed EMFs Enhances Regeneration of the Sciatic Nerve. Bioelectromagnetics.1993; 14:353-9.
Van Lunteren E, Moyer M. Wheel-running exercise alters rat diaphragm action potentials and their regulation by K channels. J Appl Physiol. 2003; 95:602-10.
Delbono O and Kotsias BA. Relation between action potential duration and mechanical activity on rat diaphragm fibers. Effects of 3,4-diaminopyridine and tetraethylammonium. Pflu gers Arch. 1987; 410:394400.
Lin-Shiau SY, Day SY, and Fu WM. Use of ion channel blockers in studying the regulation of skeletal muscle contractions. Naunyn Schmiedebergs Arch Pharmacol. 1991; 344:691-7.
Miledi R, Parker I, and Zhu PH. Extracellular ions and excitation-contraction coupling in frog twitch muscle fibers. J Physiol. 1984; 351:687-710.
Van Lunteren E, Moyer M. Electrophysiologic and inotropic effects of K-channel blockade in aged diaphragm. Am J Respir Crit Care Med. 1998; 158:820-6.
Van Lunteren E, Moyer M, Dick TE. Modulation of diaphragm action potentials by K- channel blockers. Respir Physiol. 2001; 124:217-30.
Comtois A, Light P, Renaud JM, Kong MK. Tolbutamide, but not glyburide, affects the excitability and contractility of unfatigued frog sartorius muscle. Eur J Pharmacol. 1993; 242: 65-73.
Light PE, Comtois AS, Renaud JM. The effect of glibenclamide on frog skeletal muscle: evidence for KATP channel activation during fatigue. J Physiol. 1994; 475:495-507.
Challiss RAJ, Vranic M, Radda GK. Bioenergetic changes during contraction and recovery in diabetic rat skeletal muscle. Am. J. Physiol. 1989; 256 (Endocrinol. Metab. 19): 129-37.
Kindig C, Sexton W, Fedde R, Poole D. Skeletal muscle microcirculatory structure and hemodynamics in diabetes. Respir. Physiol.1998; 111:163-75.
Sexton W, Poole D. Microcirculatory structurefunction relationships in skeletal muscle of diabetic rats. Am. J. Physiol. 1994; 266:1502-11.
Hill M, Meninger G. Impaired arteriolar myogenic reactivity in early experimental diabetes. Diabetes. 1993; 42:1226-32.
Nielson H, Bonnema S, Flyvbjerg A. Effects of diabetes, insulin treatment and osmolality on contractility of isolated rat resistance arteries. Pharmacol. Toxicol. 1995; 77:209-15.
Mcguire M, Macdermott M. The influence of streptozotocin diabetes and metformin on erythrocyte volume and on the membrane potential and the contractile characteristics of the extensor digitorum longus and soleus muscle in rats. Exp Physiol. 1999; 84:1051-58.
Pelit A, Özaykan B, Tuli A, Demirkazık A, Emre M, Gunay I. The Effects of Magnetic Field on the Biomechanics Parameters of Soleus and Extensor Digitorum Longus Muscles in Rats with Streptozotocin-Induced Diabetes. Diabetes Tech.& Ther. 2008; 10: 2.
Yazışma Adresi / Address for Correspondence: Dr. Aykut Pelit Çukurova University Medical Faculty Biophysics Department 01330 Balcali-ADANA e-mail: apelit@gmail.com; apelit@cu.edu.tr geliş tarihi/received :13.10.2012 kabul tarihi/accepted:07.12.2012