Asmada (Vitis vinifera L.) Adventif Kök Taslaklarının Farklılaşmasının İncelenmesi

Araştırma, asmada (Vitis vinifera L.) adventif köklerin gövde dokularından farklılaşmasını mikroskobik düzeyde belirlemek amacına yönelik olarak temel bir çalışma niteliğinde gerçekleştirilmiştir. Köklendirme ortamında bekletilen Cabernet Sauvignon çeşidinin çelikleri kalınlıklarına (çap 1 cm’nin altı veya üstü) göre ayrılıp bazal kısımlarından 4-5 cm uzunluğunda kesilerek FAA (formalin %10, etanol %50 ve glasiyel asetik asit %5) ortamında muhafaza edilmiştir. 60-90 μm kalınlığında kesitlere ayrılan örnekler safranin ile boyanmıştır. Adventif köklerin gövde kesitini oluşturan dokuların iki farklı bölgesinden farklılaştığı tespit edilmiştir. İnce dal parçalarında (çap1cm) ise daha içte ve öze yakın bölgelerdeki hücre gruplarından farklılaşmıştır. Düzensiz bir hücre yığını halinde oluşan kallus, kambiyumuma bitişik floem bölgesinden gelişmektedir. Birbirlerinden bağımsız şekillenen adventif kök ve kallus oluşumunun aynı zaman aralığında olduğu gözlenmiştir.

Anahtar Kelimeler:

Asma, boyama, çelik, safranin, farklılaşma

Investigation on Differentiation of Adventitious Root Primordia in Grapevine (Vitis vinifera L.)

The research was carried out as a basic study to determine localization of adventitious roots differentiated in the stemtissues of the grapevine (Vitis vinifera L.) at the microscopic level. The cuttings of Cabernet Sauvignon kept in rooting medium were grouped by their thickness (more or less than 1 cm in diameter) and 4.5 cm long basal parts were kept in FAA (formalin 10%, ethanol 50% and glacial acetic acid 5%). Samples cut into sections with a thickness of 60-90 μm were stained with safranin. It was determined that the adventitious roots are differentiated from two different regions of the stem tissues. In the thin samples (diameter 1cm) they were differentiated from the cells in more inner region close to pith. Callus, formed as an irregular cell mass, is developed from the phloem region adjacent to the cambium. Formation of adventitious root and callus, which are shaped of independently each other, is within the same time frame.

Keywords:

grapevine, staining, cutting, safranin, differentiation,

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Amissah, J.N., D.J. Jr, Paolillo and N. Bassuk. 2008. Adventitious root formation in stem cuttings of Quercus bicolor and Quercus macrocarpa and its relationship to stem anatomy. Journal of American Society of Horticultural Science, 133: 479-486. https://doi. org/10.21273/JASHS.133.4.479
Bellini, C., D.I. Pacurar and I. Perrone. 2014. Adventitious roots and lateral roots: similarities and differences. Annual Reviews of Plant Biology, 65: 639-666. https:// doi.org/10.1146/annurev-arplant-050213-035645
Bond, J., L. Donaldson, S. Hill and K. Hitchcock. 2008. Safranine fluorescent staining of wood cell walls. Biotechnic & Histochemistry, 83: 161-71. https://doi. org/10.1080/10520290802373354
Calderón Baltierra, X., G. Montenegro and E. de García. 2004. Ontogeny of in vitro rooting process in Eucalyptus globulus. In Vitro Cell Development and Biology- Plant, 40: 499-503. https://doi.org/10.1079/ IVP2004559
Favre, J.M. 1973. Effets corrélatifs de factuers internes et externs sur la rhizogenés d’un clone de vigne (Vitis riparia × Vitis rupestris) ‘in vitro’. Revue Générale de Botanique, 80: 279-361.
Favre, J.M. and R. Médard. 1969. Ontogénie des raciness adventives chez la vigne (Vitis vinifera L.) cultivée in vitro. Revue Générale de Botanique, 76: 455-467.
Filiti, N., N. Montuschi and P. Rosati. 1987. In vitro rhizogenesis: histo-anatomical aspects on Prunus rootstock. Advances in Horticultural Science, 1: 34-38.
Fujii, T. 1955. Origin and development of root primordia in grape cuttings. Journal of Japanese Society of Horticultural Science 24(3): 160-164.
Hackett, W.P. 1988. Donor plant maturation and adventitious root formation. In: Adventitious Root Formation In Cuttings. (Eds. T.D. Davis, B.E. Haissig and N. Sankhla), Dioscorides, Portland, Ore. pp 11- 28.
Han, Q-Q. 1983. The primary observation on anatomy of the adventitious root formation in grape cuttings. Journal of the Agricultural University of Hebei 6: 45- 48.
Hartmann, H.T., D.E. Kester and J.T. Davies, Jr. 1990. Plant propagation: Principles and practices. 5th ed. Prentice Hall, Englewood Cliffs, N.J.
Hayward, H.E. 1983. The Structure of Economic Plants, Mcmillan, New York, pp. 39-56.
Jásik, J. and G.J. De Klerk. 1997. Anatomical and ultrastuctural examination of adventitious root formation in stem slices of apple. Biologia Plantarum, 39(1): 79- 90. https://doi.org/10.1023/A:1000313207486
Keeley, K., J.E. Preece, B.H. Taylor and I.E. Dami. 2004. Effects of high auxin concentrations, cold storage, and cane position on improved rooting of Vitis aestivalis Michx. Norton Cuttings. American Journal of Enology and Viticulture 55(3): 265-268.
Machado, M.P, J.L.S. Mayer, M. Ritter and L.A. Biasi. 2005. Effects of indole-butyric-acid on the rooting ability of semihardwood cuttings of grapevine rootstock ‘VR 043-43’ (Vitis vinifera x Vitis rotundifolia). Revista Brasileira de Fruticultura, 27(3): 476-479. https://doi. org/10.1590/S0100-29452005000300032
Naija, S., N. Elloumi, N. Jbir, S. Ammar and C. Kevers. 2008. Anatomical and biochemical changes during adventitous rooting of apple rootstock MM 106 cultured in vitro. Comptes Rendus Biologies, 331: 518- 525. https://doi.org/10.1016/j.crvi.2008.04.002
Philippa, H., I. Bryant, J. Stephen and I. Trueman. 2015. Stem anatomy and adventitious root formation in cuttings of Angophora, Corymbia and Eucalyptus. Forests, 6: 1227-1238. https://doi.org/10.3390/ f6041227
Schumann, F. and F. Uhl. 1975. On the use of auxins favouring root formation. Mitteilungen Klosterneuburg, 25:339-346.
Van der Lek, H. 1924. Root development in woody cuttings. Mededelingen Landbouwhogeschool Wageningen 28: 211-230.