Emel OZTURK, Tufan OGE, Yunus AYDİN, Ozben ISİKLAR, Hikmet HASSA

İn vitro fertilizasyon olgularında serım ve folliküler sıvı total oksidan ve antioksidan seviyelerinin incelenmesi

Amaç: İnfertilite teşhisi konmuş hastaların İn Vitro Fertilizasyon (IVF) uygulamalarında serum ve folliküler sıvılarında total oksidan kapasite (TOC) ,total antioksidan kapasite (TAC) ve oksidatif stres indeksi (OSI) değerlerinin oosit gelişimi,fertilizasyon, embriogenezis ve klinik gebelik üzerine etkilerini değerlendirmek. Materyal Metod: Kliniğimizde IVF protokolüne alınan 100 hastanın çalışma grubunu oluşturdu ve gonodatropın başlandığı gün, embriyo transferi günü ve oosit toplandığı gün kan örnekleri alındı. İlave olarak oosit toplandığı sırada folliküler sıvı da toplandı. Serumda ve folliküler sıvıda TOC , TAC ve OSI değerleri klinik gebe olan ve olmayanlarda karşılaştırıldı. Bulgular: Gebe olan ve olmayan hastaların yaş, infertilite süresi, ovarian reserve ve transfer edilen embriyo sayıları arasında istatistiksel bir fark yok idi. Ayrıca serum TAC,TOC ve OSI değerleri arasında her üç fazda da (gonodatropın başlandığı gün, embriyo transferi günü ve oosit toplandığı gün) ve folliküler sıvıda anlamlı bir fark saptanmadı (p<0.05). Sonuç: Yardımcı üreme tekniklerine giden hastalarda TAC, TOC ve OSI değerleri klinik gebeliği öngörmede etkisiz gözükmektedir.

Anahtar Kelimeler:

Folliküler sıvı, İn vitro fertilizasyon, Total oksidan kapasite, Total antioksidan kapasite

Assessment of serum and follicular fluid total oxidant and total antioxidant levels in In Vitro Fertilization (IVF) cases

Objective: To assess the effects of serum and follicular fluid total oxidant (TOC) levels, total antioxidant capacity (TAC), and oxidative stress index (OSI) on oocyte maturation, fertilization, embryogenesis, and clinical pregnancy outcomes in In Vitro Fertilization (IVF) cycles of infertile patients.Materials and Methods: One hundred patients having infertility and underwent ART enrolled the study group and blood samples were collected on gonadotropin starting, oocyte pick-up (OPU) and embryo transfer (ET) days. Additionally, follicular fluid specimen obtained during OPU was collected. TOC, TAC levels and OSI in serum samples and follicular fluid specimens between clinically pregnant and non-pregnant patients were compared.Results: No significant difference was noted between clinically pregnant and nonpregnant patients in terms of the woman's age, duration of infertility, ovarian reserve or number of transferred embryos. There was also no significant difference in TAC, TOC and OSI levels in serum samples obtained during three phases of treatment cycle (basal gonadotropin starting day, OPU day and ET day) and in follicular fluid samples. Conclusion: TAC, TOC and OSI seem to be ineffective to predict clinical pregnancy as an outcome in patients who underwent ART.

Keywords:

Follicular Fluid, Infertility, Total Oxidant Capacity, Total Antioxidant Capacity,

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