Bu çalışmada, farklı fiksasyon metotlarının koyun ve rat paratiroidlerinin ultrastrüktürü üzerine etkisi incelendi, immersiyon-fiksasyonla tespit edilen koyun paratiroid (PT) bezlerinin elektron mikroskopisinde, multi-nükleer hücrelerde düzensiz şekilli çekirdeklerin bir birine çok yakın olduğu ve aralarında plazma membran kalıntılarının bulunduğu belirlendi. Kromatinin, çekirdek içinde ince grandiler ve düzensiz parçacıklar şeklinde dağıldığı görüldü. Yoğunluğu az olan sitoplazmik matrikste, az sayıda salgı granülüne ve Golgi kompleksinin küçük keseciklerine rastlandı. Endoplazmik retikulum sisternaları ve mitokondriyonların oldukça fazla şekilde dilate olduğu tesbit edildi. Perfüzyon-fiksasyonla tespit edilen rat PT bezlerinin elektron mikroskop incelenmesinde ise, multi-nükleer hücreler yerine uniform hücreler gözlendi. Bu hücrelerde plazma membranının kıvrımlı ve sitoplazmik matriksin yoğun olduğu belirlendi. Endoplazmik retikulum sisternaları, Golgi kompleks kesecikleri ve mitokondriyonların önemsiz derecede dilate olduğu ve salgı grandilerine çok az rastlandığı tesbit edildi. Bu bulgulardan, koyun paratiroid bezlerinde multi-nükleer hücrelerin oluşumunun farklı fiksasyon metotlarından kaynaklanabileceği sonucuna varıldı.
The effects of different fixation methods on the ultrastructure of parathyroid cells was studied in sheep and rats. Electron microscopy of parathyroid (PT) glands of sheep fixed by immersion demonstrated multi-nucleated cells with close to each other irregularly shaped nuclei showing folds and undulations, with remnants of the plasma membrane between them. The chromatin is finely granular and dispersed over the nucleus in irregular clusters. The electron-light cytoplasmic matrix containing few secretory granules (SG), small Golgi complex (GC), rough endoplasmic reticulum (RER) with dilated cisternae were indicated. Mitochondria were markedly swollen. In contrast, the PT glands of the rats fixed by perfusion exhibited uniform cells instead of multi-nuclear cells. The uniform cells containing electron-dense cytoplasmic matrix, tortuous plasma membranes, slightly dilated RER and GC, few SG and inconspicuous dilated mitochondria were observed. From these finding we conclude that the occurrence of the "multi-nucleated cells" in PT glands of sheep depends on fixation methods.
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