Hindi lenfoid organlarında (Timus, ve Bursa Fabricius) yaşa bağlı olarak mast hücrelarinin dağılımı

Bu çalısma, hindilerde bazı lenfoid organlarda yasa baglı olarak mast hücrelerinin dagılımının belirlenmesi amacıyla yapıldı. Arastırmada, yörede aynı kosullarda yetistirilen 15, 30 ve 120 günlük yaslarında ve her yas grubunda 6 adet olmak üzere toplam 18 Bronz hindi kullanıldı. Hindiler dekapite edilerek timus, dalak ve bursa Fabricius’dan doku örnekleri alındı. Alınan örnekleri Mota’nın bazik kursun asetat (BLA) tespit solüsyonunda tespit edildi. Mast hücrelerin demonstrasyonu için toluidine blue ve Alcian blue/safranin O boyama metotları kullanıldı. Timusda tüm yas gruplarında medullada korteksten daha fazla mast hücresi oldugu belirlendi. Timus ve bursa Fabricius’da 15. günde en yüksek seviyede olan mast hücre sayısı, 30 ve 120. günlerde anlamlı olarak düsüs gösterdi (P

Anahtar Kelimeler:

mast hücreleri, yaş, fabricius kesesi, lenfatik sistem, hindi, dalak, kümes hayvanları, heterojenlik

Age-related distribution and heterogeneity in the number of Mast cells lymphoid of the organs in Turkey

This study was carried out to determine the distribution of mast cells in the lymphoid organs of turkey. The materials were obtained from a local turkey farm. A total of 18 Bronze turkeys were divided into three groups according to their ages (15, 30 and 120-days-old). After having been sacrified by decapitation, the turkeys’ thymus, spleen and bursa of Fabricius tissue samples were taken. Tissues were fixed in Mota’s fixative (BLA) for 24 h and embedded in paraffin. The histologic sections were stained with toluidine blue and alcian blue – safranine O in order to determine the mast cells distribution. In all age groups, MC density was higher in medulla than cortex in the thymus. Mast cells were the highest at the 15-days-old group, however, the numbers were significantly decreased in the thymus and bursa of Fabricius at 30 and 120-day-old group (P<0.05). At the 30-days-old group, the number of mast cell in spleen was higher than at 15-days-old but was lower than at 120-days-old group. Safranine positive MCs were not observed in all organs and in all age groups. These results showed age-related changes in the number of MCs of the lymphoid tissues in turkey.

Keywords:

mast cells, age, bursa fabricii, lymphatic system, turkeys, spleen, poultry, heterogeneity,

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Yüzüncü Yıl Üniversitesi Veteriner Fakültesi Dergisi-Cover

ISSN: 1017-8422
Yayın Aralığı: Yılda 3 Sayı
Başlangıç: 1990
Yayıncı: Yüzüncü Yıl Üniv. Veteriner Fak.

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