Servet KILIÇ, Ökkeş YILMAZ, Cafer Tayar İŞLER, Sami ÜNSALDI

Effect of xylazine-thiopental anesthesia on canine fatty acid profiles

Bu çalışmada 5 kontrol grubu (KG) ve 5 ksilazin-tiopental grubu (KTG) köpekte yağ asitlerinin tip ve konsantrasyon profili araştırıldı. KG’u köpeklerin kan örnekleri alındıktan sonra tiopental ile ötenazi edilip böbrek ve karaciğerlerinden doku örnekleri alındı. KTG’u olgular i.m. 1.5 mg/kg ksilazin hidroklorid ve i.v 20 mg/kg tiopental sodyum verilerek aneztezi edildiler. KTG’u olgular anesteziden tamamen çıkıp kan örnekleri alındıktan sonra tiopental verilerek ötenazi edilip karaciğer ve böbreklerinden doku parçası alındı. Alınan tüm örnekler gaz kromotografisi kullanılarak analiz edildiler. Analiz sırasında KG’unun serum, karaciğer ve böbreklerinden sırasıyla 12, 17 ve 18, KTG’unun serum, karaciğer ve böbreklerden 13, 19 ve 17 farklı tipte yağ asidi saptandı. KTG’unun verileri KG’nununki ile karşılaştırıldığında böbreklerdeki palmitik asit (C16:0) ve stearik asit (C18:0) ile karaciğerdeki linoleik asit (C18:2n-6) verilerinde azalma, buna karşın karaciğerdeki eikosatrienoik asit (C20:3n-9) miktarında belirgin bir artış saptandı. Ayrıca KTG’unun serum, karaciğer ve böbreklerindeki toplam yağ asidi konsantrasyonunun KG’undakilerden yüksek olduğu fakat bu yüksekliğin istatsitiksel olarak önemli olmadığı gözlemlendi. Sonuç olarak, mevcut verilerden ksilazin-tiopentalın yağ aistleri profili üzerine hücresel düzeyde etki oluşturabileceği kanısına varıldı.

Köpeklerde ksilazin-thiopental anestezisinin yağ asidi profili üzerine etkisi

In this study, the profiles of fatty acid types and concentrations were investigated on 5 control group (CG) and xylazine-thiopental group (XTG) dogs. In CG, after obtaining the blood samples, the dogs were killed with thiopental application and then the tissue sections from their livers and kidneys were harvested. All XTG dogs were anesthetized with i.m. administration of 1.5 mg/ kg xylazine hydrochloride and i.v. administration of 20 mg/kg thiopental sodium. After collecting blood samples following full anesthetic recovery they were killed with overdose thiopental application, then tissue sections from their livers and kidneys were taken. All samples were analyzed with a gas chromatograph. During this investigation we found 12, 17 and 18 different types of FAs in serum, liver and kidney tissues of CG and 13, 19 and 17 in serum, liver and kidney tissues of XTG, respectively. When XTG data were compared with those of CG, a significant decrease in palmitic acid (C16:0) and stearic acid (C18:0) data of kidney and linoleic acid (C18:2n-6) data of liver, whereas an increase in eicosatrienoic acid (C20:3n-9) data of the liver were determined. It was also detected that total FA concentrations of serum, liver and kidney in XTG was lower than CG, but this reduction was not statistically significant. In conclusion, the present results suggest that thiopental-xylazine may have some effects on FA profiles at cellular level.

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