Türkiye’de Baklagil Nodüllerinden İzole Edilen Bitki Gelişimini Destekleyici Aktinobakterilerin Filogenisi

Aktinobakteriler, tarımsal önemi olan moleküllerin yanı sıra birçok biyoaktif metabolitin üretimindeki işlevlerinden dolayı yararlanılan, biyoteknolojik olarak önemli bir mikroorganizma grubudur. Bu çalışmada, yabani baklagil nodüllerinden kültüre dayalı bir yaklaşımla aktinobakteriler izole edilmiş ve indol-3-asetik asit üretimi, atmosferik azot fiksasyonu ve inorganik fosfatı çözebilme özellikleri açısından bitki gelişimini destekleyici aktiviteleri araştırılmıştır. İzolatları tanımlamak amacıyla 16S rRNA gen dizi analizine dayanan bir moleküler yaklaşım kullanılmıştır. İkili dizi analizleri sonrasında altı izolatın Streptomyces ve Micromonospora cinslerinin üyeleri olduğu tespit edilmiştir. Bütün izolatlar indol-3-asetik asit üretebilmiş ve atmosferik azotu kullanabilmiştir. Ancak sadece bir izolat inorganik fosfatı çözünür hale getirebilmiştir. İzole edilen bu aktinobakteriler, özellikle atmosferik azotu kullanabilmeleri ve indol-3-asetik asit üretebilmeleri nedeniyle umut verici biyolojik gübre adayları olarak değerlendirilmektedir.

Anahtar Kelimeler:

Actinobacteria, 16S rRNA, İndolasetik asit, Lathyrus, Vicia

Phylogeny of Plant Growth-Promoting Actinobacteria Isolated from Legume Nodules in Turkey

Actinobacteria are a biotechnologically important group of microorganisms utilized for their high capacity to synthesize many bioactive substances as well as agriculturally important compounds. In the present study, a culture-dependant approach was employed to isolate actinobacteria from wild legume nodules and their plant growth-promoting activities for indole-3-acetic acid production, atmospheric nitrogen fixation and inorganic phosphate solubilisation was investigated. A molecular approach based on 16S rRNA gene sequence analysis was employed to identify the isolates. After pairwise sequence analysis, six isolates were identified as members of the genera Streptomyces and Micromonospora. All isolates could produce indole-3-acetic acid and utilize atmospheric nitrogen while only one isolate was able to solubilize inorganic phosphate. The isolated actinobacteria are considered to be promising candidates for biological fertilizers especially because of their ability to use atmospheric nitrogen and produce high level of indole-3-acetic acid.

Keywords:

Actinobacteria, 16S rRNA, Indoleacetic acid, Lathyrus, Vicia,

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