SP-2 (Prunus spinosa) Klonal Anaç Adayının in vitro Rejenerasyonu Üzerine Farklı Bitki Büyüme Düzenleyici Konsantrasyonlarının Etkileri

Bu çalışma, Kahramanmaraş doğal ortamından seleksiyon ıslahıyla elde edilen klonal anaç adayı SP-2 (Prunus spinosa) (Uğur ve Paydaş Kargı 2017)’nın doku kültürüyle üretilebilme olanaklarının araştırılması amacıyla 2017 yılında yürütülmüştür. Çalışmada, MS (Murashige and Skoog) besi ortamı ve bu ortama ilave edilen iki farklı hormon ve bitki büyüme düzenleyici kombinasyonları MS-1 (TDZ 0.75 mg/l, NAA 0.5 mg/l, GA3 0.5 mg/l, 0.5 mg/l) MS-2 (TDZ 1.5 mg/l, NAA 1.5 mg/l, GA3 0.5 mg/l, 0.5 mg/l ), kontrol olarak ise MS-0 (Hormonsuz normal MS) kullanılmıştır. Çalışma sonunda explant başına en yüksek kardeş bitkicik sayısının MS-1 ortamından (12.60 adet/explant) ve en uzun sürgün uzunluğunun 14.19 mm değeriyle MS-0 ortamından elde edildiği saptanmıştır. Çalışma sonunda en yüksek kardeşlenme oranının elde edildiği ve daha az miktarda hormonun kullanıldığı MS1 ortamının, MS2 ortamına göre daha başarılı ve ekonomik olduğuna karar verilmiştir.

Anahtar Kelimeler:

Anaç, Erik, in vitro, Prunus spinosa

Effects of Different Concentrations of Plant Growth Rugulators on in vitro Regeneration of SP-2 (Prunus spinosa) Clonal Candidate Rootstock

Progagation possibilities in vitro conditions with applied plant growth regulators concentration of SP-2 (Prunus spinosa) (Ugur and Paydas Kargi 2017) candidate rootstock which obtained by selection breeding programm in Kahramanmaras natural environment was determined in this study at 2017 year. MS (Murashige and Skoog) medium, and this medium added two different plant growth regulator combinations MS-1 (TDZ 0.75 mg L-1, NAA 0.5 mg L-1, Ga3 0.5 mg L-1 0.5 mg L-1) MS-2 (TDZ 1.5 mg L-1, NAA 1.5 mg L-1, Ga3 0.5 mg L-1, 0.5 mg L-1) and MS-0 (normal MS without any hormone) as control was used material. Maximum cumulated shoots per explant at the MS-1 medium (12.60 pieces explant-1) and longest shoot lenght at the MS-0 medium (14.19 mm) was found.It was decided that would be MS-1 medium more economic than MS-2 medium because less amount of hormones used.

Keywords:

Rootstock, Plum, in vitro, Prunus spinosa,

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