Identification and Characterization of Fluorescent Pseudomonas Producing Active Compounds Controlling Plant Pathogens

Identification and Characterization of Fluorescent Pseudomonas Producing Active Compounds Controlling Plant Pathogens

Fluorescent pseudomonad is one of the biocontrol agents against plant pathogens. Various compounds reportedly can be produced by fluorescent pseudomonad, including chitinase, β-1,3-glucanase, HCN, siderophore, antibiotics, Indole Acetic Acid (IAA), phosphate solvent compounds, and 2,4-diacetylphloroglucinol (DAPG). In this study, it was identified and characterized six isolates of fluorescent pseudomonad (PfPj1, PfPj2, PfKd7, PfCas, PfCas3, and LAHp2). All isolates were isolated from the rhizosphere of various types of plants. The results showed that six isolates were identical to Pseudomonas aeruginosa (93-94%). All bacterial isolates tested were able to produce siderophore, HCN, and solubilize phosphates. The highest siderophore was produced by isolate PfPj2. Whereas isolate PfKd7 had the highest at HCN production and the ability to dissolve phosphates.

___

  • Advinda, L. (2009). Physiological Response of Banana Plants Introduced by the Fluorescent Pseudomonad Formula Against Blood Disease Bacteria (BDB). Doctoral Thesis (PhD), Universitas Andalas, Padang, Indonesia.
  • Advinda, L., Fifendy, M., & Anhar, A. (2018). The addition of several mineral sources on growing media of fluorescent pseudomonad for the biosynthesis of hydrogen cyanide. IOP Conf. Series: Materials Science and Engineering, 335. doi:10.1088/1757-899X/335/1/012016.
  • Advinda, L., Pratama, I., .Fifendy, M., Anhar, A., & Armaleni. (2019). The addition of various carbon sources on growing media to increase the siderophore level of fluorescent pseudomonad bacteria. IOP Conf. Series: Journal of Physics: Conf. Series, 1317. doi:10.1088/1742-6596/1317/1/012078.
  • Ahemad, M., & Kibret, M. (2014). Mechanisms and applications of plant growth promotingrhizobacteria: Current perspective. Journal of King Saud University – Science, 26, 1-20.
  • Ahmed, E & Holmström, S.J.M. (2014). Siderophores in environmental research: roles and applications. Microb Biotechnol., 7(3): 196-208.
  • Alizadeh, O. (2011). Effect of Plant Growth Promoting Bacteria on Crop Growth. American-Eurasian Journal of Sustainable Agriculture, 5(3): 344-349.
  • Anhar, A., Doni, F & Advinda, L. (2011). Response of Rice Plant Growth (Oryza Sativa L.) Against the introduction of fluorescent pseudomonad. Eksakta, 1(12).
  • Anitha, G & Kumudini, B.S (2014). Isolation and characterization of fluorescent pseudomonads and their effect on plant growth promotion. Journal of Environmental Biology, 35, 627-634.
  • Cody, Y.S & Gross, D.C. (1987). Characterization of Pyoverdinpss, the Fluorescent Siderophore Produced by Pseudomonas syringae pv. syringae. Applied and Environmental Microbiology, 53(5): 928-934.
  • Deshwal, V.K & Kumar, P. (2013). Plant growth promoting activity of Pseudomonads in Rice crop. Int.J.Curr.Microbiol.App.Sci, 2(11): 152-157.
  • Elad, Y & Baker R. (1985). The role of competition for iron and carbon in suppression of chlamydospore germination of Fusarium spp. by Pseuydomonas spp. Phytopathology, 75:1053-1059.
  • Karthikeyan, M., K. Radhika., S. Mathiyazhagan., R. Bhaskaran., R. Samiyappan & Velazhahan. (2006). Induction of phenolics and defense-related enzymes in coconut (Cocos nucifera L.) roots treated with biocontrol agents. Braz. J. Plant Physiol., 18(3), 367-377.
  • Kumar Aj, Kumar Am, Devi S, Patil S, Payal C & Negi S. (2012). Isolation, screening and characterization of bacteria from Rhizospheric soils for different plant growth promotion (PGP) activities: an in vitro study. Recent Research in Science and Technology, 4(1): 01-05.
  • Maheswar, N.U. & Sathiyavani, G. (2012). Solubilization of phosphate by Bacillus sps, from groundnut rhizosphere (Arachis hypogaea L.). Journal of Chemical and Pharmaceutical Research, 4(8): 4007-4011.
  • Maleki, M., Mostafaee, S., Mokhtarnejad, L., & Farzaneh, M. (2010). Characterization of Pseudomonas fluorescens strain CV6 isolated from cucumber rhizosphere in Varamin as a potential biocontrol agent. Australian Journal of Crop Science AJCS, 4(9):676-683.
  • Nguyen, M.T., & Ranamukhaarachchi, S,L. (2010). Soil-borne Antagonists for Biological Control of Bacterial Wilt Disease Caused by Ralstonia solanacearum in Tomato and Pepper. Journal of Plant Pathology, 92(2): 385-395.
  • Olanrewaju, O.S., Glick, B.R., & Babalola, O.O. (2017). Mechanisms of action of plant growth promoting bacteria. World J Microbiol Biotechnol, 33:197.
  • Paul, D. & Sarma, Y.R. (2006). Antagonistic effects of metabolites of Pseudomonas fluorescens strains on the different growth phases of Phytophthora capsici, foot rot pathogen of black pepper (Piper nigrum L.). Archives of Phytopathology and Plant Protection Month, 39(0): 1-6.
  • Podile, A.R & G.K. Kishore. (2006). Plant growth-promoting rhizobacteria. In: Plant-Associated Bacteria (Ed: S.S. Gnanamanickam). Springer. Printed in the Netherlands. pp. 195–230.
  • Prasad, M.R., Sagar, B.V., Devi, G.U., Triveni, S., Koteswar-Rao, S. R. & Chari, K.D. (2017). Isolation and Screening of Bacterial and Fungal Isolates for Plant Growth Promoting Properties from Tomato (Lycopersicon esculentum Mill.). Int.J.Curr.Microbiol.App.Sci, 6(8): 753-761.
  • Qessaoui, R., Bouharroud, R., Furze, J.N., El Aalaoui, M., Akroud, H., Amarraque, A., VanVaerenbergh, J., Tahzima, R., Mayad, E.H., & Chebli, B. 2019. Applications of New Rhizobacteria Pseudomonas Isolates in Agroecology via Fundamental Processes Complementing Plant Growth. Scientific Reports, 9:12832. Doi: https://doi.org/10.1038/s41598-019-49216-8.
  • Reetha, S., G. Bhuvaneswari, P. Thamizhiniyan & T. Ravi Mycin. (2014). Isolation of indole acetic acid (IAA) producing rhizobacteria of Pseudomonas fluorescens and Bacillus subtilis and enhance growth of onion (Allium cepa L.). Int.J.Curr.Microbiol.App.Sci, 3(2): 568-574.
  • Salaheddin, K., Valluvaparidasan, V., Ladhalakshmi, D., & Velazhahan, R. (2010). Management of bacterial blight of cotton using a mixture of Pseudomonas fluorescens and Bacillus subtilis. Plant Protect.Sci., 46: 41–50.
  • Subramanian, J. & Satyan, K. (2014). Isolation and selection of fluorescent pseudomonads based on multiple plant growth promotion traits and siderotyping. Chilean Journal of Agricultural Research, 74(3).
  • Vanitha, S. & Ramjegathesh, R. (2014). Bio Control Potential of Pseudomonas fluorescens against Coleus Root Rot Disease. J Plant Pathol Microb, 5: 216.
  • Viveros, O.M., Jorquera, M.A., Crowley, D.E., Gajardo, G., & Mora, M.L. (2010). Mechanisms and Practical Considerations Involved in Plant Growth Promotion by Rhizobacteria. J. Soil Sci. Plant Nutr. 10 (3): 293-319.
  • Vyas, P. & Gulati, A. (2009). Organic acid production in vitro and plant growth promotion in maize under controlled environment by phosphate-solubilizing fluorescent Pseudomonas. BMC Microbiology, 9:174.
  • Widnyana, I.K. & Javandira, C. (2016). Activities Pseudomonas spp. and Bacillus sp. to Stimulate Germination and Seedling Growth of Tomato Plants. Agriculture and Agricultural Science Procedia, 9: 419-423.
  • Yadav, A., Yadav, K., & Vashistha, A. (2016). Phosphate solubilizing activity of Pseudomonas fluorescens PSM1 isolated from wheat rhizosphere. Journal of Applied and Natural Science, 8(1): 93-96.
  • Yanti, Y., Habazar, T., Reflinaldon., Nasution, C.R., & Felia, S. (2017). Indigenous Bacillus spp. ability to growth promoting activities and control bacterial wilt disease (Ralstonia solanacearum). Biodiversitas, 18(4): 1562-1567.
  • Yeole, R.D. & Dube, H.C. (2001). Siderophore production by fluorescent pseudomonads colonizing roots of certain crop plants. Indian Journal of Experimental Biology, 39(5):464-468.
Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi-Cover
  • ISSN: 1308-7576
  • Başlangıç: 1991
  • Yayıncı: Yüzüncü Yıl Üniversitesi Ziraat Fakültesi
Sayıdaki Diğer Makaleler

Investigations of Some Morphological, Pomological, and Physiological Parameters with Mineral Content of Different Rosa L. Taxa Grown under Greenhouse Condition

Ibrahim Halil HATİPOGLU, Bekir Erol AK, Birgül DİKMETAŞ, Meral DOĞAN

Cations-Base Application in Rubber Plantation: The Change of Calcium, Magnesium, and Potassium Status in the Soil and Leaves and Its Relation to Latex Yield

Irwan Agusnu PUTRA, Hamidah HANUM, Radite TISTAMA, Edison PURBA

Identification of Paddy Rice Diseases Using Deep Convolutional Neural Networks

Hakkı Fırat ALTINBİLEK, Ünal KIZIL

Assessment of climate variability and the determinants of rice productivity in Southeastern Nigeria

Chinedu MBA, Christopher EZEH, Ignatius A. MADU, Chukwudi EMERİBE

Genetic Diversity and Phylogenetic Position of Traditional Rice (Oryza sativa L.) Landraces: A Case Study of South Kalimantan in Indonesia

Dindin Hidayatul MURSYİDİN

Extraction and Identification of Volatile Compounds in Rosa laxa Retz var harputense T. Baytop "Kişmiri rose"

Şevket ALP, Mozhgan ZARIFIKHOSROSHAHI, Gülşah YAŞA ÖZTÜRK, Sezai ERCİSLİ

Significant Natural Wound Healing Agents: Herbs and Single Bioactive Principles

Sayed FİRDOUS, Marwa FAYED, Varnita KARMAKAR, Ratna MANDAL, Syeda FATHİMA

Study on Correlation of Agromorphologic Properties in Some Camelina (Camelina sativa (L.) CRANTZ.) Genotypes

Yusuf ARSLAN, İlhan SUBAŞI, Halil HATİPOĞLU, Servet ABRAK, Berfin İŞLER

Determination of the Effect of Salt Stress on Germination, Biochemical and Antioxidant Defense Systems in Linas Safflower Seeds

Civan ÇELİK, Yaşar KARAKURT

Sex Reversal of the Giant Freshwater Prawn Macrobrachium dacqueti through Androgenic Gland Ablation

Roy VİLLANUEVA, Princess TABERNA, Marjorie VİLLANUEVA, Jaro AJIK, Albaris TAHILUDDIN