Nazife EROĞLU, Merve KAMBUR, Meral KEKEÇOĞLU

Farklı Bal Arısı Irklarının Propolis Toplama Tercihlerinin Araştırılması

Bu çalışmada farklı arı ırklarının propolis toplamak için farklı bitki kaynaklarını tercih edip etmediği araştırılmıştır. Bu amaçla Türkiye’de doğal olarak bulunduğu bilinen 4 farklı bal arısı ırkı (Apis mellifera caucasica, A. m. carnica, A. m. syriaca and A. m. anatoliaca) izole edilmiş aynı arılığa yerleştirilmiş ve bu ırklardan propolis hasadı yapılmıştır. Hasat edilen propolislerin alkolik ekstraksiyonları yapılarak sıvı kromatogram kütle spektrofotometrisi (LCMS/MS) ile kimyasal içerikleri analiz edilmiştir. Aynı zamanda aynı propolislerin mikroskop ile polen içeriği belirlenmiştir. Elde edilen sonuçlara göre farklı bal arısı ırklarının topladığı propolisler kuersetin ve ferulik asit bakımından önemli düzeyde farklılık göstermiştir. Farklı ırklardan elde edilen propolis örneklerinde en fazla Fabaceae and Apiaceae (PD >45%) familyasından polenlere reslanırken, Campanulaceae familyasından polene yalnızca A. m. anatoliaca ırkının topladığı polenlerde ve Caryophyllaceae familyasından polenlere ise sadece A. m. caucasica ırkının topladığı propolislerde raslanmıştır. Bu çalışmanın sonuçları farklı arı ırklarının farklı bitki kaynaklarına yönelme davranışında bulunduğunu ve ırka göre propolis içeriğinin değişebileceğini göstermiştir

Anahtar Kelimeler:

Anadolu, Bal arısı ırkları, Propolis

The Investigation Propolis Foraging Preference of Different Honey Bee

In this study, it was investigated whether different bee breeds prefer different plant sources to collect propolis. For this purpose four different honey bee race (Apis mellifera caucasica, A. m. carnica, A. m. syriaca and A. m. anatoliaca) naturally have been in Turkey were placed in the same isolated apiary; and Propolis was harvested from these races. chemical contents of alcoholic extractions of the harvested propolis were were analyzed by liquid chromatography-tandem mass spectrometry (LCMS / MS). In addition that, the pollen content of the same propolis samples were determined with a microscope. According to the LCMS / MS results the propolis samples collected by different honey bee race differed significantly in terms of quercetin and ferulic acid. Data obtained from polen analyses revealed that Fabaceae and Apiaceae (PD >45%) families were mostly detected in propolis samples obtained from different races. Although the polen from the Campanulaceae family was dedected only in the propolis samples from A. m. anatoliaca race, thr polen from Caryophyllaceae family was found in other propolis samples collected by A. m caucasica races. The results of this study showed that different honey bee races tend to different plant sources and the content of propolis may differ according to the bee races.

Keywords:

Anatolia, honey bee races, propolis,

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