Mehmet EZER, Fatma YILMAZ ERTÜRK, Hatice BEŞEREN, Yasemen ADALI

Mesane ürotelyal karsinomlarında Cyclin-D1 immünohistokimyasal ekspresyonunun araştırılması

Amaç:Cyclin D1 hücre siklusunun düzenlenmesinde görev alan ve CCND1 geni tarafından kodlanan bir proteindir. Liteatürde Cyclin D1 ile ilgili az sayıda çalışma mevcut olup çalışmalarda sonuçlar farklılık göstermektedir. Bu çalışmada mesane üretelyal karsinomlarda Cyclin D1ekspresyonunun prognostik faktörler ile ilişkisi araştırıldı. Gereç ve Yöntem: Çalışmaya Kafkas Üniversitesi Sağlık Araştırma ve Uygulama Hastanesi’nde TUR-M yapılmış 46 olgu dahil edildi. Olgulara dair genel bilgiler ve patoloji raporları hastane otomasyon sisteminden elde edildi. Hazır boyalı patoloji preparatlarından tümör içeren kesitler seçilip seçilen kesitlere ait bloklara Cyclin D1 primer antikoru ile immunohistokimyasal boyama aşağıda belirtilen şekilde manuel olarak yapılmıştır.Boyanan kesitler ışık mikroskobunda nükleer ve sitoplazmik olarak ayrı ayrı 0-4 olarak skorlanarak değerlendirildi. Bulgular: Olguların yaş aralığı 51-93 olup ortalama yaş 69.2±11.7’dir. Olguların 12’si (%26.1) kadın, 34’ü (%73.9) erkektir. Histopatolojik bulgular incelendiğinde olguların 29’unun (%63.0) düşük dereceli 17’sinin (%37.0) yüksek dereceli olduğu gözlenmiştir. Olguların 18’i (%39.1) invaziv, 28’i (%60.9) noninvaziv niteliktedir. Yapılan istatistiksel analizlerde invazyon gösteren tümörlerin non-invaziv tümörlere göre (pTa) istatistiksel olarak anlamlı düzeyde yüksek dereceli olduğu dikkati çekmiştir (p= 0.007). Benzer şekilde invaziv tümörlerde lenfovasküler invazyon varlığı non-invaziv tümörlere göre istatistiksel olarak anlamlı derecede fazla saptanmıştır (p=0.001). Nükleer cyclin D1 expresyonunun (p=0.003) invaziv olgularda istatistiksel olarak anlamlı düzeyde daha yüksek olduğu izlenmiştir. Ayrıca düşük dereceli tümörlerde nükleer cyclinD1 ekspresyonu istatistisel olarak anlamlı düzeyde yüksek bulunduğu saptanmıştır (p=0.044). Sonuç: Çalışma sonucunda mesane üretelyal karsinomlu hastalarda Cyclin D1 ekspresyonun tümöe derecesi ve invazyon durumu ile ilişkisi gözlenmiştir ancak Cyclin D1’in biyobelirteç olarak kullanılabilmesi için daha geniş olgu serilerinde çalışmalara ihtiyaç duyulmaktadır.

Anahtar Kelimeler:

Cyclin D1, mesane, üretelyal karsinom, immünohistokimya

Investigation of Cyclin-D1 immunohistochemical expression in bladder urethelial carcinoma

Objective: Cyclin D1 is a protein that is involved in the regulation of the cell cycle and is encoded by the CCND1 gene. There are few studies on Cyclin D1 in the literature, and the results differ in the studies. In this study, the relationship between Cyclin D1 Expression and prognostic factors in bladder urothelial carcinomas was investigated. Materials and Methods: Forty-six patients who underwent TUR-M at the Kafkas University Health Research and Application Hospital were included in the study. General information about the cases and pathology reports were obtained from the hospital automation system. Tumor-containing sections were selected from the ready-stained pathology preparations, and immunohistochemical staining was performed manually with Cyclin D1 primary antibody on the blocks of the selected sections, as indicated below. Stained sections were evaluated under the light microscope by scoring 0-4 separately as nuclear and cytoplasmic. Results: The age range of the cases was 51-93, and the mean age was 69.2±11.7. Twelve (26.1%) cases were female and 34 (73.9%) were male. When the histopathological findings were examined, it was observed that 29 (63.0%) of the cases were low-grade and 17 (37.0%) were high-grade. Eighteen (39.1%) of the cases were invasive and 28 (60.9%) were noninvasive. In the statistical analyzes performed, it was noted that invasive tumors had a statistically significantly higher grade compared to non-invasive tumors (pTa) (p= 0.007). Similarly, the presence of lymphovascular invasion in invasive tumors was statistically significantly higher than in non-invasive tumors (p=0.001). It was observed that nuclear cyclin D1 expression (p=0.003) was statistically significantly higher in invasive cases. In addition, nuclear cyclinD1 expression was found to be statistically significantly higher in low-grade tumors (p=0.044). Conclusion: As a result of the study, a relationship between Cyclin D1 expression and tumor grade and invasion status was observed in patients with bladder urethelial carcinoma, but studies with larger case series are needed to use Cyclin D1 as a biomarker.

Keywords:

bladder, Cyclin D1, urothelial carcinoma, immunohistochemistry,

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