Kamelia STAMATOVA YOVCHEVA, Rosen DIMITROV, David YOVCHEV, Diyana VLADOVA, ÖMER GÜRKAN DİLEK, Radoslav MIHAYLOV

Histological definition for the gray scale ultrasonography of the rabbit liver

Bu çalışmanın amacı, tavşan karaciğerinin morfolojik ve histolojik özelliklerinin uygun anatomik ultrason (USG) görüntüsünün elde edilmesi için referans teşkil edebileceğini kanıtlamaktır. Bu amaçla, çalışmada 12 adet klinik olarak sağlıklı Yeni Zelandalı tavşanı kullanılmıştır. Histolojik incelemede, Hematoksilen / Eozin rutin boyama yöntemi kullanılmıştır. Ultrason çalışması ise 2D görselleştirme için ultrasonik ekipmanlarla gerçekleştirilmiştir. Tavşan karaciğerinin ultrason görüntüsü, organı oluşturan dokuların farklı akustik empedansı ile üretilmiştir. Tavşan karaciğerinin anatomik USG görüntüsü gözlemlenirken oluşan gri ve beyaz nüansların değişkenliği ise histolojik özellikleri yardımıyla üretilmiştir. Karaciğerin USG akustiğinde USG dalgasında aynı şiddette değişkenlik gözlemlendi. Bu durumun karaciğer ve safra kanalının histolojik özelliklerine bağlı olarak şekillendiği düşünülmektedir. Tavşan karaciğerinin USG görüntülemesi, parankim, perivasküler bağ dokusu ve ekstrahepatik safra yolları tarafından üretilen yankı sinyalinin dağılma karakteri nedeniyle gerçekleşmektedir. Fibröz yapıdaki kapsülün ve karaciğer parankiminin farklı akustiği; parlaklık ve kontrast, gri-beyaz ölçeğe göre, çeşitli gri nüans ve USG dalgasının hızı gibi USG indeksleriyle ilgilidir. Dolayısıyla bu çalışmada çalışılan organın USG morfolojik karakteri, histolojik özellikleri ile tanımlanmakta olduğu sonucuna varılabilir. Karaciğerin bu histolojik özellikleri, USG'nin organın anatomik olarak görselleştirilmesini tanımladığı için "Altın Standart" olarak kabul edilebilir

Tavşan karaciğerinin gri skala ultrasonografsi için histolojik olarak tanımlanması

The aim of the present study is to prove that the morphological and histological features of the rabbit liver are base for the creation of proper anatomical US image. For the purpose, we use 12 clinically healthy New White Zealand rabbits. In the histological study, we use the routine staining with Hematoxylin/Eosin. The US study was carried out with ultrasonic equipment for 2D visualization. The US image of the rabbit liver was produced by the different acoustic impedance of the tissues, which composed the organ. The variability of the grey and white nuances when observing the anatomical US image of the rabbit liver is produced by its histological features. It is not relative to the orientation of the transducer to the field of study. There was a variability of the US acoustics of the liver at the same intensity of the US wave. This is also owing to the histological features of the liver and biliary ducts. US visualization of the rabbit liver is because of the dispersion character of the echo-signal, generated by parenchyma, perivascular connective tissue and extrahepatic biliary ducts. The different acoustics of capsula fibrosa and liver parenchyma is related to the following US indices: brightness and contrast, in accordance to the greywhite scale, a variety of the grey nuance and speed of the US wave. We present the following conclusion: The US morphological character of the studied organ is defned by its histological features. These histological features of the liver could be accepted as “Golden standard”, because they defne the US anatomical visualization of the organ

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