Oksijen Radikal Absorbans Kapasitesi (ORAK) Seviyeleri Farklı Bitki Masere Yağlarının Yoğun Stoklanmış Gökkuşağı Alabalıklarının (Oncorhynchus mykiss) Bazı Kan Parametrelerine Etkileri

Bu çalışmada, oksijen radikal absorbans kapasiteleri (ORAK) farklı bitkilerin [soğan (Allium cepa), sarımsak (Allium sativum), reyhan (Ocimum basilicum), haşhaş (Papaver somniferum), zencefil (Zingiber officinale), zerdeçal (Curcuma longa)], masere yağları elde edilmiştir. Çalışma, kontrol grubu (ayçiçek yağı) dahil olmak üzere toplam 7 grupta, bir hafta boyunca 30 litrelik havlandırmalı tanklarda, su sirkülasyonu olmadan gerçekleştirilmiştir. Her tanka 20 adet (14,6±1,0 gr) gökkuşağı alabalığı yüksek yoğunlukta stoklanmıştır. Masere yağlar, ilk gün balığın lateral çizgisi boyunca derisine sürülmüştür. Masere yağların su ve balık üzerine etkilerini belirlemek için suyun Oksidasyon-redüksiyon potansiyeli (ORP) ve gökkuşağı alabalıklarının hematolojik parametreleri [granulosit (GRAN), beyaz kan hücresi (WBC), kırmızı kan hücresi (RBC), hemoglobin (HGB), kırmızı kan hücresindeki ortalama hemoglobin miktarı (MCH), eritrositlerin hacmindeki değişikliğin standart sapması (RDW-SD), trombositlerin ortalama büyüklüğü (MPV), kan hücrelerindeki trombositlerin dağılım aralığı (PDW), büyük hücreli trombosit oranı (P-LCR)] balıklarda incelenmiştir. Sonuçlar suyun ORP seviyesinde azalmalar olduğunu göstermiştir. En yüksek yaşama oranı haşhaş yağı uygulamasında olduğu belirlenmiştir. 7 günlük uygulama sonunda, en yüksek WBC, LYM, HGB, MCV, MCH, MPV değerleri zencefil yağı uygulamasında (ORAK: 28.811) belirlenmiştir. En yüksek RBC değeri zerdeçal yağı (ORAK: 159.277) uygulamasında, en yüksek GRAN değeri haşhaş yağı (ORAK: 481) uygulamasında, en yüksek HCT ve PLT değerleri reyhan yağı (ORAK: 67.553) uygulamasında olduğu tespit edilmiştir. Çalışma sonunda masere yağların deriye sürülmesinin kan parametrelerini uyardığı tespit edilmiştir.

Anahtar Kelimeler:

Masere yağ, Gökkuşağı alabalığı, Oncorhynchus mykiss, Oksijen radikal absorbans kapasitesi (ORAK), Oncorhynchus mykiss

Effects of Different Plant Oils Having Different Oxygen Radical Absorbance Capacity (ORAC) on Hematological Parameters of Rainbow Trout (Oncorhynchus mykiss) at High Stocking Density

In this study, macerated oils of different plant [sunflower (Helianthus annuus), onion (Allium cepa), garlic (Allium sativum), basil (Ocimum basilicum), opium poppy (Papaver somniferum), ginger (Zingiber officinale), turmeric (Curcuma longa)] were obtained. The study was conducted at a total of 7 groups, including the control group (sunflower oil), in 30 liter aerated tanks without water circulation during a week. Twenty rainbow trout per tank (14.6±1.0 g) were stocked at high density. Macerated oils were applied to the skin of the fish along the lateral line on the first day. For determination of effects of macerated oils on water and fish, oxidation-reduction potential (ORP) of water and hematological parameters [granulocyte (GRAN), white blood cell (WBC), red blood cell (RBC), hemoglobin (HGB), mean corpuscular hemoglobin (MCH), red cell distribution width (RDW-SD), mean platelet volume (MPV), platelet distribution width (PDW), platelet large cell ratio (P-LCR)] were examined in fish. The results indicated that decreases were determined in ORP level of water. It was determined that the highest survival rate was in the application of basil oil. At the end of 7 days, the highest WBC, LYM, HGB, MCV, MCH, MPV values were determined in the application of ginger oil (ORAC: 28.811). It was determined that the highest RBC value was in the application of turmeric oil (ORAC: 159.277), the highest GRAN value was in the application of poppy oil (ORAK: 481), the highest HCT, PLT values was in the application of basil oil (ORAC: 67.553). At the end of the study, it was determined that the macerated oil application to lateral line induced blood parameters.

Keywords:

Macerated oil, Oncorhynchus mykiss, Oxygen Radical Absorbance Capacity (ORAC), Rainbow trout,

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