Serkan YILDIRIM, Saadet BELHAN, Hasan UYAR, Zübeyr HUYUT, Gökhan OTO, Yavuz Selim SAĞLAM

Kronik Olarak Flor ve 7,12-Dimetilbenzantrasene (DMBA) Maruz Kalmanın Ratlarda Spermatogenezis ve Testisin Histopatolojisine Etkileri

Bu çalışmada, sıçanlarda florür ve polisiklik aromatik hidrokarbon (PAH) türevi olan 7,12-dimetilbenz [a] antrasen (DMBA)'in spermatogenesis ve testisin histopatolojisi üzerine tek tek ve kombinasyon halinde etkilerinin araştırılması amaçlandı. 250-300 g ağırlığında 40 erkek Wistar albino sıçanı kullanıldı. Deneyin 90. gününde intrakardiak kan örnekleri ve testis doku örnekleri alındı. Gruplarda ki FSH ve LH düzeylerinde her hangi bir değişiklik gözükmezken testosteron düzeylerinin kontrol grubuna göre diğer gruplarda önemli ölçüde düştüğü belirlendi (P<0.001). Kronik flor ve DMBA toksikasyonu ratlarda sperm motilite oranını, yoğunluğunu önemli ölçüde düşürmekte ve anormal sperm oranı ise yükseltmektedir. Histopatolojik incelemelerde; NaF (30 ppm) grubunda ki ratların testislerinin tubulus duvarında spermatosit ve spermatozoa sayısında azalma görüldü. DMBA (10 mg/kg) grubunda bulunan ratların testis dokuları incelendiğinde, tubuluslarda atrofi, intertubuler aralıklarda ödem ve tubulus duvarında bulunan spermatosit sayısında şidettli azalma tespit edildi. NaF (30 ppm) + DMBA (10 mg/kg) grubunda ki ratların testis dokuları incelendiğinde ise, seminifer tubullerde atrofi, tubullerin duvarında spermatogoniumlarda şiddetli dejeneratif ve nekrotik değişiklikler ve buna bağlı olarakta tubul duvarlarında incelme saptandı. Tubul lümenlerinde spermatozoa rastlanmadı. İntertubuler aralıklarda şiddetli ödem, bu bölgelerde bulunan damarlarda dilatasyon ve hiperemi saptandı. Sonuç olarak NaF ve DMBA nın testis dokusu ve döl verimi üzerine her birinin tek tek olumsuz etki ettiği ve birlikte kombinasyon halinde ise bu etkinin daha da şiddetlendiği görüldü.

Anahtar Kelimeler:

Rat, Spermatogenezis, Kronik Florozis, 7-12-dimethylbenzanthracene (DMBA), Testis

Effects of Chronically Exposure to Flor and 7,12-Dimethylbenzanthracene (DMBA) on Spermatogenesis and Testicular Histopathology in Rats

This study aimed to investigate the effects of both sodium fluoride (NaF) and polycyclic aromatic hydrocarbon (PAH)derivative such as 7,12-dimethylbenz[a]anthracene (DMBA) singly and in combination on spermatogenesis and testishistopathology in rats. Forty male Wistar albino rats weighing 250–300 g used. On the 90th day of the experiment,intracardiac blood samples and testes were taken. While there was no change in FSH and LH levels in the groups, testosteronelevels were significantly decreased in the other groups according to the control group (P<0.001). It was decreased the ratioof motility of sperm, sperm density and, increased the abnormal sperm rate by chronic fluorosis and DMBA toxicity in ratssignificantly. In histopathological examinations; A decrease in the number of spermatocytes and spermatozoa in the tubuluswall of the testes of rats in the NaF (30 ppm) group was observed. When testicular tissues of rats in the DMBA (10 mg / kg)group were examined, atrophy in tubulus, edema in intertubular spaces and a decrease in the number of spermatocytes inthe tubulus wall were found. When testes tissues of rats in the group of NaF (30 ppm) + DMBA (10 mg / kg) were examined,atrophy in seminiferous tubules, severe degenerative and necrotic changes in spermatogoniums on the walls of tubules andthinning in tubule walls were observed. There is not found spermatozoon in the tubul lumens. Severe edema in theintertubular spaces, dilatation and hyperemia in the veins found in these regions. As a result, it was observed that NaF andDMBA had a negative effect on testicular tissue and fertility singly, and in combination, this effect was further exacerbated.

Keywords:

Chronic Fluorosis, 7-12-dimethylbenzanthracene (DMBA), Rat, Spermatogenesis, Testis,

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