Gözde YAMAN BÜLBÜL, Leyla MİS, Emin ŞENGÜL, Serkan YILDIRIM, Fikret ÇELEBİ, Ali ÇINAR

Cyclophosphamide İle İndüklenmiş Ratlarda Karaciğer Enzimleri (AST, ALT, ALP) ve Histopatolojisi Üzerine Naringin’in Protektif Etkileri

Bu çalışmada Cyclophosphamide (CYP) uygulaması yapılan ratlarda karaciğer enzimleri (Aspartat aminotransferaz (AST), Alanin aminotransferaz (ALT), Alkalen fosfataz (ALP) ve histopatolojisi üzerine Naringin’in protektif etkilerinin araştırılması amaçlanmaktadır. Araştırmamızda yaklaşık 200-250 g ağırlığında, 40 adet erkek erişkin Sprague Dawley ırkı rat kullanıldı ve 5 grup oluşturuldu. Karaciğer enzimlerinin analizleri otoanalizörde ve spektrofotometrede yapıldı. Hematoksilen eosin ile boyanan karaciğer doku örnekleri ışık mikroskobunda incelendi. CYP’nin ratlarda hepatotoksiteye yol açtığı ve karaciğer enzimlerini artırdığı görüldü. AST, ALT ve ALP değerleri Kontrol grubunda 58±11, 32±8, 38±6; CYP grubunda 237±42, 168±44, 74±11; Naringin 50+CYP grubunda 223±33, 158±42, 62±9; Naringin 100+CYP grubunda 117±25, 107±24, 48±8; Naringin 100 grubunda ise 54±9, 31±7, 36±6 sırasıyla olarak bulundu. Kontrol grubundan elde edilen değerlere göre CYP verilen gruplarda ki AST, ALT ve ALP değerlerinin tamamında P<0.001 düzeyinde artış söz konusudur. CYP’nin karaciğer dokusunda oluşturduğu dejeneratif ve nekrotik doku hasarının Naringin’in etkisiyle dozlara bağlı olarak azaldığı saptandı. Sonuç olarak, Naringin’in farklı dozlarının, CYP ile indüklenen hepatotoksisitede protektif etkili olduğu belirlendi.

Anahtar Kelimeler:

Cyclophosphamide, Histopatoloji, Karaciğer Enzimleri, Naringin, Rat

Protective Effects of Naringin on Liver Enzymes (AST, ALT, ALP) and Histopathology in Cyclophosphamide-Induced Rats

In this study, it is aimed to investigate the protective effects of Naringin on liver enzymes (Aspartate Aminotransferase (AST), Alanine Aminotransferase (ALT), Alkaline Phosphatase (ALP) and histopathology in CYP-induced rats. In our study, forty male adult Sprague Dawley rat weighing approximately 200-250 g were used and 5 gropus formed. Analyzes of liver enzymes were done in auto analyzer and spectrophotometer. The liver tissue samples stained with hematoxylin eosin were examined by light microscopy. CYP was found to cause hapatotoxicity and showed increased liver enzymes in rats. AST, ALT and ALP values were 58±11, 32±8, 38±6 in the control group; 237±42, 168±44, 74±11 in the CYP group; 223±33, 158±42, 62±9 in the Naringin 50+CYP group; 117±25, 107±24, 48±8 in Naringin 100+CYP group; 54±9, 31±7, 36±6 in the group of Naringin 100 were found respectively. According to the values obtained from the control group, there is an increase of P<0.001 in all of the AST, ALT and ALP values in CYP-treated groups. Degenerative and necrotic tissue damage caused by CYP in the liver tissue was found to be reduced as dose-dependent with effect of Naringin. In conclusion, different doses of Naringin were determined to be protective effective for CYP-induced hepatotoxicity.

Keywords:

Cyclophosphamide, Histopathology, Liver Enzeymes, Naringin, Rat,

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