Bakır Nefrotoksisitesinde Melatonin ve E Vitamini’nin Antioksidan Parametreler Üzerine Etkileri

Bakır (Cu) bileşikleri özellikle endüstri ve tarımda geniş kullanım alanına sahiptir. İz miktardaki Cu hayatın sürdürülebilirolması için gerekliyken fazlası organizma için toksik etki yapmaktadır. Bu çalışma akut Cu toksisitesinde melatonin ve Evitamini’nin böbreklerdeki antioksidan enzim aktivitelerine etkisinin araştırılması amacıyla planlanmıştır. Çalışmada 28 Wistaralbino sıçanlar kontrol grubu, Cu grubu, Cu+E vitamini grubu ve Cu+Melatonin grubu şeklinde eşit dört gruba (n=7) ayrıldı.Böbrek dokusunda süperoksit dismutaz (SOD), glutatyon peroksidaz (GPx), katalaz (KAT) enzim aktiviteleri ve protein karboniliçeriği analiz edildi. Ayrıca böbrek fonksiyon testleri değerlendirildi. Bakır uygulaması, kreatinin, kan üre nitrojeni (BUN), ürikasit, Na, K ve Mg değerlerini artırırken, E vitamini ve melatonin bu değerlerin azalmasında etkili oldu. Karbonil seviyesi, Cu+Evitamini veya Cu+melatonin gruplarında Cu grubuna göre önemli derecede azaldı. Doku SOD, GPx ve KAT aktiviteleri Cumaruziyeti sonucu düşmesine rağmen; E vitamini veya melatonin uygulaması, bu aktivitelerde önemli bir artışa neden oldu.Sonuç olarak, E vitamini ve melatonin takviyesi antioksidan sistemi güçlendirerek Cu temelli oksidasyonun engellenmesinisağlayabilir. Bu sayede nefrotoksisitenin gelişmesini önleyerek koruyucu bir fonksiyon gösterebilir.

Anahtar Kelimeler:

Antioksidan, E vitamini, Melatonin, Oksidatif stres, Protein oksidasyonu, Protein oksidasyonu

The Effects of Melatonin and Vitamin E on Antioxidant Parameters in Copper Nephrotoxicity

Copper (Cu) compounds have been widely used in various fields especially industrial processes and agriculture.Although trace amounts of Cu are essential to sustain life, excessive Cu is toxic for living organisms. This study aims toinvestigate the effects of vitamin E and melatonin on antioxidant enzymes activities in kidney against acute Cu toxicity. In thisstudy, 28 Wistar albino rats were divided four equal groups (n=7) as control, Cu, Cu+Vitamin E, and Cu+Melatonin groups.Superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) enzymes activities and protein carbonyl contentwere analyzed. Also, kidney function tests were evaluated. While Cu administration increased creatinine, blood urea nitrogen(BUN), uric acid, Na, K and Mg levels, vitamin E and melatonin was effective reduction on these levels. Carbonyl levelsignificantly decreased in Cu+Vitamin E and Cu+melatonin groups compared to Cu group. Although SOD, GPx and CATactivities in tissue decreased by Cu exposure, vitamin E and melatonin treatment showed significant increase on theiractivities. Consequently, vitamin E and melatonin supplements may provide prevention Cu -based oxidation by strengtheningthe antioxidant defense system. In this respect, it may show a protective effect by preventing the development ofnephrotoxicity.

Keywords:

Antioxidant, Melatonin, Oxidative stress, Protein oxidation, Vitamin E.,

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