Achillea millefolium (Civanperçemi) Ekstraktı ve Luteolin’in İn Vitro Rat Mesanesi Düz Kas Kontraksiyonları Üzerine Etkileri

Civanperçemi (CP) alternatif tıpta yaygın olarak kullanılmakta ve yapısında birçok bileşikle birlikte Luteolin debulunmaktadır. Bu çalışmada, CP metanol ekstraktı ve Luteolin’in izole rat mesane düz kas kontraktilitesi üzerine etkileriaraştırıldı. Çalışmada 20 adet erkek Sprague Dawley rat kullanıldı ve ratlar anestezi altında dekapite edildiler. İzole edilenmesanelerin temizlikleri yapıldı ve mesanelerden hazırlanan kas şeritleri organ banyosuna asıldı. Dokuya 1 gram gerimuygulandı ve 1 saatlik inkübasyon periyoduna bırakıldı. İnkübasyon periyodu sonunda banyoya CP ekstraktı, Luteolin,asetilkolin (ACh), potasyum klorür (KCl) ve Nifedipin bir protokol çerçevesinde uygulandı. Veriler one way ANOVA’da Tukeytesti ile değerlendirildi. CP ekstraktı ve Luteolin’nin farklı dozlarının, ACh ve KCl ile indüklenen izole mesane kontraksiyonlarınıinhibe ettikleri belirlendi. ACh’nın submaksimal dozu ile indüklenen kontraksiyonların banyoda Nifedipin varlığında CPekstraktı ve Luteolin ile daha güçlü bir şekilde baskılandığı görüldü. KCl’nin submaksimal dozu ile indüklenen kontaksiyonlarıCP ekstraktı ile Nifedipin’in birlikte daha fazla inhibe ettikleri belirlendi, ancak banyoda Nifedipin varlığında Luteolin’inetkisinin değişmediği tespit edildi. Bu çalışmada CP ekstraktı ve Luteolin’nin farklı dozlarının mesane düz kas kontraksiyonlarınıinhibe ettikleri ve CP ekstraktı ve Luteolin’in L tipi Ca2+ kanalları aracılığıyla etkili olurken, CP ekstraktının ayrıca M3 reseptöryolağı üzerinden de etkili olabileceği belirlendi.

Anahtar Kelimeler:

Civanperçemi, Luteolin, Mesane, Rat., Rat.

The Effects of Achillea millefolium (Yarrow) Extract and Luteolin on In Vitro Rat Urinary Bladder Muscles

Yarrow (CP) is commonly used in alternative medicine and in its structure is found Luteolin together with manycompounds. In this study, effects of CP methanol extract and Luteolin on isolated rat bladder smooth muscle contractilitywere investigated. In the study, twenty male Sprague Dawley rats were used and the rats were decapitated under anesthesia.The cleaning of isolated bladders were done and muscle strips prepared from the bladders were placed to organ baths. Thetissues were applied to 1 gram tesnion and left for 1 hour incubation period. At the end of the incubation period, CP extract,Luteolin, acetylcholine (ACh), potassium chloride (KCl) and Nifedipine was applied with a protocol the bath. Data wereevaluated by Tukey test in one way ANOVA. It was determined that different doses of CP extract and Luteolin inhibited theisolated bladder contractility induced by ACh and KCl. It was seen that of the contractions induced by ACh submaximal dosemore strongly suppressed by CP extract and Luteolin in the presence of Nifedipine in the bath. Subcutaneous dose-inducedindications of KCl were found to be more inhibitory in co-existence of CP extract and Nifedipine. It was determined thattogether with CP extract and Nifedipine were more inhibited the KCl’s submaximal dose-induced contractions, but but theeffect of Luteolin in the presence of Nifedipine in bath was not changed. In this study, it was assigned that inhibiting thebladder smooth muscle contractions of different doses of CP extract and Luteolin and of CP extract to be also effective on theM3 receptor pathway.

Keywords:

Luteolin, Rat, Urinary bladder, Yarrow,

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1. Passalacqua N., Guarrera P., De Fine G., 2007. Contribution to the knowledge of the folk plant medicine in Calabria region (Southern Italy). Fitoterapia, 78, 52-68.
2. Pieroni A., Quave CL., 2005. Traditional pharmacopoeias and medicines among Albanians and Italians in southern Italy: A comparison. J Ethnopharmacol, 101, 258-270.
3. Judzentiene A., 2016. Atypical Chemical Profiles of Wild Yarrow (Achillea millefolium L.) Essential Oils. Rec Nat Prod, 10, 262-268.
4. Nematy M., Mazidi M., Jafari A., Baghban S., Rakhshandeh H., Norouzy A., Esmaily H., Etemad L., Patterson M., Mohammadpour AH., 2017. The effect of hydro-alcoholic extract of Achillea millefolium on appetite hormone in rats. Avicenna J Phytomed, 7, 10-15.
5. Lakshmi T., Geetha R., Roy A., Aravind Kumar S., 2011. Yarrow (Achillea millefolium Linn.) A Herbal Medicinal Plant with Broad Therapeutic Use–A Review. Int J Pharm Sci Rev Res, 9, 136-41.
6. Nemeth E., Bernath J., 2008. Biological activities of yarrow species (Achillea spp.). Curr Pharm Des, 14, 3151-3167.
7. Barut N., Barut B., Engin S., Yıldırım S., Yaşar A., Türkiş S., Özel A., Sezen FS., 2017. Antioxidant capacity, anti-acetylcholinesterase activity and inhibitory effect on lipid peroxidation in mice brain homogenate of Achillea millefolium. Turk J Biochem, 42, 493-502.
8. Ahmadi-Dastgerdi A., Ezzatpanah H., Asgary S., Dokhani S., Rahimi E., 2017. Phytochemical, Antioxidant and Antimicrobial Activity of the Essential Oil from Flowers and Leaves of Achillea millefolium subsp. millefolium. J Essent Oıl Bear Pl, 20, 395-409.
9. Yaeesh S., Jamal Q., Khan AU., Gilani AH., 2006. Studies on hepatoprotective, antispasmodic and calcium antagonistactivities of the aqueous–methanol extract of Achillea millefolium. Phytother Res, 20, 546-551.
10. Khan AU., Gilani AH., 2011. Blood pressure lowering, cardiovascular inhibitory and bronchodilatory actions of Achillea millefolium. Phytothe Res, 25, 577-583.
11. Ali SI., Gopalakrishnan B., Venkatesalu V., 2017. Pharmacognosy, Phytochemistry and Pharmacological Properties of Achillea millefolium L.: A Review. Phytother Res, 31, 1140-1161.
12. Borrelli F., Romano B., Fasolino I., Tagliatatela‐Scafati O., Aprea G., Capasso R., Capasso F., Coppola Bottazzi E., Izzo A., 2012. Prokinetic effect of a standardized yarrow (Achillea millefolium) extract and its constituent choline: studies in the mouse and human stomach. J Neurogastroenterol Motil, 24,164-190.
13. Benedek B., Gjoncaj N., Saukel J., Kopp B., 2007. Distribution of phenolic compounds in middleeuropean taxa of the Achillea millefolium L. aggregate. Chem Biodivers, 4, 849-857.
14. Barnes J., Anderson LA., Phillipson JD., 2003. Herbal Medicines: A Guide for Healthcare Professionals. 2th ed., Pharmaceutical Press, London.
15. Lemmens-Gruber R., Marchart E., Rawnduzi P., Engel N., Benedek B., Kopp B., 2006. Investigation of the spasmolytic activity of the flavonoid fraction of Achillea millefolium on isolated guinea-pig ilea. Arzneimittelforschung, 56, 582-588.
16. Babaei M., Abarghoei ME., Akhavan MM., Ansari R., Vafaei AA., Taherian AA., Mousavi S., Toussy J., 2007. Antimotility effect of hydroalcoholic extract of yarrow (Achillea millefolium) on the guinea-pig ileum. Pak J Biol Sci, 10, 3673-3677.
17. Packer L., Sies H., 2001. Flavonoids and Other Polyphenols: Methods in Enzymology. 1st ed., Academic Press, Cambridge, Massachusetts.
18. Kasala ER., Bodduluru LN., Barua CC., Gogoi R., 2016. Antioxidant and antitumor efficacy of Luteolin, a dietary flavone on benzo (a) pyrene-induced experimental lung carcinogenesis. Biomed Pharmacother, 82, 568-577.
19. Naso LG., Lezama L., Valcarcel M., Salado C., Villacé P., Kortazar D., Ferrer EG., Williams PA., 2016. Bovine serum albumin binding, antioxidant and anticancer properties of an oxidovanadium (IV) complex with luteolin. J Inorg Biochem, 157, 80-93.
20. Lemanska K., van der Woude H., Szymusiak H., Boersma MG., Gliszczynska-Swiglo A., Rietjens IM., Tyrakowska B., 2004. The effect of catechol O-methylation on radical scavenging characteristics of quercetin and Luteolin-A mechanistic insight. Free Radic Res, 38, 639-647.
21. Qi L., Pan H., Li D., Fang F., Chen D., Sun H., 2011. Luteolin improves contractile function and attenuates apoptosis following ischemia–reperfusion in adult rat cardiomyocytes. Eur J Pharmacol, 668, 201-207.
22. Philyppov IB., Paduraru ON., Gulak KL., Skryma R., Prevarskaya N., Shuba YM., 2016. TRPA1-dependent regulation of bladder detrusor smooth muscle contractility in normal and type I diabetic rats. J Smooth Muscle Res, 52, 1-17.
23. Şengül E., Çelebi F., Gelen V., Çınar A., 2017. The effects of rosmarinus officinalis (rosemary) aqueous extract on smooth muscle contractions in ısolated rat urinary bladder. Atatürk Üniversitesi Vet Bil Derg, 12, 130-136.
24. Karaki H., Ozaki H., Hori M., Mitsui-Saito M., Amano KI., Harada KI., Miyamoto S., Nakazawa H., Won KJ., Sato K., 1997. Calcium movements, distribution, and functions in smooth muscle. Pharmacol Rev, 49, 157-230.
25. Anderson KE., 1993. Pharmacology of lower urinary tract smooth muscles and penile erectile tissues. Pharmacol Rev, 45, 253-308.
26. Moradi M., Rafieian-Koupaei M., Imani-Rastabi R., Nasiri J., Shahrani M., Rabiei Z., Alibabaei Z., 2013. Antispasmodic effects of yarrow (Achillea millefolium l.) extract in the isolated ileum of rat. Afr J Tradit Complement Altern Med, 10, 499-503.
27. Abrams P., Andersson KE., Buccafusco JJ., Chapple C., Groat WC., Fryer AD., Kay G., Laties A., Nathanson NM., Pasricha PJ., 2006. Muscarinic receptors: Their distribution and function in body systems, and the implications for treating overactive bladder. Br J Pharmacol, 148, 565-578.
28. Chess‐Williams R., 2002. Muscarinic receptors of the urinary bladder: detrusor, urothelial and prejunctional. Auton Autacoid Pharmacol, 22, 133-145.
29. Andersson KE., Wein AJ., 2004. Pharmacology of the lower urinary tract: basis for current and future treatments of urinary incontinence. Pharmacol Rev, 56, 581-631.
30. Niazmand S., Saberi Z., 2010. The chronotropic and inotropic effects of aqueous-ethanolic extract of Achillea millefolium on rat’s isolated heart. Pharmacologyonline, 3, 791-798.
31. Van Den Broucke C., Lemli J., 1983. Spasmolytic activity of the flavonoids from Thymus vulgaris. Pharm Weekbl, 5, 9-14.
32. Wang L., Han C., Wang P., 1986. Experimental study on hypotensive effect of semi-synthesis luteolin. Chin Pharmacol Bull, 2, 34-36.
33. Wang L., Han C., Wang P., 1992. Experiment research on luteolin affecting coronary circulation dynamics. Chin Pharmacol Bull, 8, 388-390.
34. Chan EC., Pannangpetch P., Woodman OL., 2000. Relaxation to flavones and flavonols in rat isolated thoracic aorta: mechanism of action structure-activity relationships. J Cardiovasc Pharmacol, 35, 326-333.
35. Duarte J., Vizcaíno FP., Utrilla P., Jiménez J., Tamargo J., Zarzuelo A., 1993. Vasodilatory effects of flavonoids in rat aortic smooth muscle. Structure-activity relationships. Gen Pharmacol: The Vascular System, 24, 857-862.
36. Jiang H., Xia Q., Wang X., Song J., Bruce IC., 2005. Luteolin induces vasorelaxion in rat thoracic aorta via calcium and potassium channels. Pharmazie - Int J Pharm Sci, 60, 444-447.
37. Fleer H., Verspohl E., 2007. Antispasmodic activity of an extract from Plantago lanceolata L. and some isolated compounds. Phytomedicine, 14, 409-415.