Hale ERGİN EĞRİTAĞ, Oğuz MERHAN, Kadir BOZUKLUHAN, Kemal VAROL, Türker ATCALI

Investigation of serum leptin, ghrelin, irisin, insulin levels and their correlations in cattle with subclinical ketosis

In this study, it was aimed to investigate the correlations with leptin, ghrelin, irisin and insulin levels in the blood serum of cattle with subclinical ketosis. For this purpose, 10 healthy and 10 Holstein cattle with subclinical ketosis obtained from farms in Burdur region were used. A diagnosis of subclinical ketosis was made according to the Rothera test performed in milk, by performing a general clinical examination of the animals. Blood samples were taken from vena jugularis into tubes without anticoagulant. Serum leptin, ghrelin, irisin and insulin levels were measured in the obtained sera using commercial ELISA kits. In cows with subclinical ketosis, serum ghrelin, leptin, irisin and insulin values were increased compared to the control group (p<0.05). In the correlation findings, a highly and quite significant positive correlation was found between serum ghrelin and serum irisin values (r= 0.802; p<0.001). A moderately and quite significant positive correlation was found between plasma ghrelin value and plasma insulin value (r=0.673; p=0.001). A moderately and significant positive correlation was determined between plasma ghrelin value and plasma leptin value, between plasma irisin value and plasma leptin value, and between plasma irisin value and plasma insulin value (r=0.623; p=0.003; r=0.474; p= 0.035; r=0.558; p=0.011). In conclusion, in this study, correlations were observed between serum levels of leptin, irisin, ghrelin and insulin hormones in animals with subclinical ketosis. However, it is thought that leptin, ghrelin, insulin and irisin hormones, which are associated with lipid and carbohydrate metabolism, can be used as important biomarkers in the diagnosis of subclinical ketosis and in the follow-up of its prognosis.

Keywords:

ghrelin insulin, irisin, ketosis, leptin, negative energy balance,

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