Sıçanlarda Yüksek Oranda Doymuş Yağlı Diyet ile Aralıklı Beslemenin Deneysel Kolon Gelişimi ve Bazı Serum İnflamasyon Markırları Üzerine Etkisi 1 Adiponektin ve Lipid Metabolizması

Bu çalışmada yüksek oranda yağlı diyetle beslenen ve kolonotropik kanserojen maddeye maruz bırakılan ratlarda, haftada sadece 2 gün tam gıda kısıtlamasının (modifiye aralıklı besleme) serum adiponektin ve lipit metabolizması üzerine etkileri incelendi. Wistar albino ratlar her iki grupta 15 rat olacak şekilde deney (aralıklı besleme) ve kontrol olmak üzere iki gruba ayrıldı. Her iki grupta da yüksek yağlı diyetle 2 hafta ön besleme uygulaması sonrasında 10 deneme haftası boyunca yüksek yağlı beslemeye devam edildi. Deney grubu haftada sadece 2 gün tamamen yemden uzak tutuldu. Her iki gruba da deneme başında kolonotropik kimyasal karsinojen olan Dimetilhidrazin (DMH) 2 doz halinde 25 ve 125 mg/kg subkutan enjeksiyon şeklinde yapıldı. Çalışma sonunda serumda adiponektin, trigliserit, kolesterol, Vldl ve Hdl parametrelerine bakıldı. Deney grubunda adiponektin, kolesterol ve Vldl değerleri kontrol grubuna göre yüksek, trigliserit (p

Anahtar Kelimeler:

Yüksek Yağlı Diyet, Adiponektin, Trigliserit Obezite, Rat

The Effect of a Highly Saturated Fat Diet and Intermittent Fasting Diet on Experimental Colon Cancer Development and Some Serum Inflammation Markers in Rats, 1 Adiponectin and Lipid Metabolism

In this study, the effects of two-days food restriction per week (intermittent feeding) on the serum adiponectin and lipid profiles in rats subjected to high fat diet and exposed to Dimetilhidrazin (DMH), a potent colon carcinogen, were investigated. The Wistar albino rats were divided into two groups as experimental and control. After two weeks period of pre-feeding with high fat diet for adaptation and adjustment, the both groups were fed with the same diet for a course of 10 weeks. Experimental group was deprived of food twice a week only. Both groups received two doses of Dimethylhydrazine (DMH), 25 and 125 mg/kg, a chemical colonotropic carcinogen, at the beginning of the experiment. At the end of the study, adiponectin, triglyceride, cholesterol, VLDL and HDL were measured. In the experimental group, adiponectin, cholesterol and VLDL levels were found to be higher than control group, though not statistically significant, but triglyceride levels were significantly lower than the control group (p<0.05). However, HDL levels were lower than the control group insignificantly. As a result, this study suggest that it could be possible to modulate obesity, which is a chronic inflammation state and trigger of many metabolic abnormalities, and to prevent some metabolic pathologies by just 2 days intermittent fasting regime without stopping high fat diet feeding

Keywords:

Highly fat diet Adiponectin, Trigliserid, Obesity, Rat,

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