Nasreldin HASSAN AHMED, Nedžad GRADAŠČEVİĆ, Muhamed KATİCA

In Vitro Ortamda Kalsiyum Klorürün Osteoblast Benzeri Hücreler Üzerine Etkisi

Bu çalışmada in vitro ortamda kalsiyum klorürün osteoblast benzeri hücrelerin proliferasyonu üzerine cinsiyet bağımlı olarak etkisi olup olmadğının belirlenmesi ve osteoblast benzeri hücrelerin proliferasyonlarını etkileyen en uygun konsantrasyonun belirlenmesi amaçlanmıştır. Her iki cinsiyetten 90- 95 günlük ratların kemik ilikleri çalışmanın biyolojik materyalini oluşturmuştur. Altı farklı kalsiyum klorür konsantrasyonu test edildi ve 24 ve 48 saat sonra osteoblast benzeri hücrelerin sayısal değerleri tespit edildi. 24 saat sonunda erkekler ve dişiler arasındaki ortalama değerlerin test sonuçları, 0.25 mM ve 1.8 mM kalsiyum klorür konsantrasyonları istatistiki olarak anlamlı derecede farklılık göstermiştir(P

Anahtar Kelimeler:

Kemik iliği, kalsiyum klorür, hücre proliferasyonu, osteoblast

Influence of Calcium Chloride on Osteoblast Like Cells of Both Sexes in Rats in In Vitro Conditions

The aim of the study was to determine whether calcium chloride affects the proliferation of osteoblast like cells in a sex-dependent manner, as well as to determine the most effective concentration on proliferation of osteoblast like cells, in in vitro conditions. Bone marrow was used as biological material from young adult rats, both sexes, aged 90-95 days. Six different concentrations of calcium chloride were tested, determining the numerical representation of osteoblast like cells after 24 and 48 hours. Test results of mean values between males and females after 24 hours, indicate significant differences with a probability of P

Keywords:

osteoblast, bone marrow, Calcium chloride, Cell proliferation,

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