ANTIOXIDANT ACTIVITY of DIFFERENT EXTRACTS FROM STALK PART of RHUB ARB (RHEUM RHABARBARUM L.)
Bu çalış
mada, ravent (Rheum rhabarbarum L.)saplarının metanol, etil asetat ve heksan ekstraktlarının antioksidan
aktiv itele ri farklı in vitro model
sistemle ri ku llan ıla rak değerlendirilmiştir.Ravent saplarının polar çözücüdeki ekstraktlarının
serbest radikal süpürme aktiv
itele ri, 2,2'-a zino-b is (3- etilbenzotia
zo lin-6-sülfon ik asit),
hidro ksil ve 1,1-difenil-2-p ikrilh idra
zil radika lle ri kullanılara k
ayrı ayrı ölçülmüştür. Her bir ekstraktın
demir iyonlarını şelatlama kabiliyetleri ve toplam
antioksidan kapasiteleri araştırılmıştır. Buna ek olarak, ravent sap
ekstraktlarının toplam fenolik, flavonoid ve flavonol içerikleri be lirlen
miştir. Metanol ekstresi, en yüksek1,1-difenil-2-pikrilhidrazil radikalini temizleme
aktivitesini göstermişte olup, ardından etilasetat
vehekzan ekstreleri
ve
son olarak
da
bütil hidroksitoluen yer
almaktaydı. Etil asetat ekstraktı,
en yüksek 2,2'-azino-bis (3-etilbenzotiazolin-6-sülfonik asit) radika
l temizle me aktiv itesini gösterirken (IC50
değeri 59,7±1,1 μg/mL), bunu bütil hidroksi toluen ve metanol
ekstraktı takip etmekteydi (sırasıyla53.1±2.2 ve 47,9±1,3
μg/mL), ancak hidroksilradikalini temizleme aktivitesini en fazla metanol
ekstraktı göstermekteydi (IC50
değeri
75.1±2.9 μg/mL). Toplam antioksidan
aktivite, en fazla metanol ve etilasetat ekstraktlarında bulunmuştur
(1 mg ekstrakta sırasıyla 284,1±11,6 ve 237,6±10 μg askorbik asit eşdeğeri).
100 μg/ mL konsantrasyonundaki metanol ve etilasetat ekstraktları, demir
iyonlarına karşı en yüksek şelatlama akt ivitesini göstermişlerd ir (sırasıy la
% 96,3±2,2 ve % 89,8±1,5). Ravent sap ekstraktları, kullanılan çözücüye ve
konsantrasyona bağlı olarak oldukça belirgin bir antioksidan aktivite
göstermiştir. Ravent sap ekstraktlarının güçlü bir antioksidan
potansiyel göstermesi, Rheum rhabarbarum L.’nin
ümit verici bir bitki olduğunu düşündürmektedir.
Anahtar Kelimeler:
Rheum rhabarbarum L., ravent, sap, antioksidan aktivite
In this study, the antioxidant
activities of methanol, ethyl acetate and hexane extracts from stalks of
Rhubarb (Rheum rhabarbarum L.) were evaluated using different model systems
ivitro. Free radical scavenging activities were assessed by measuring the
scavenging activities of stalks different polar e xt racts separately using
2,2'-azino-bis (3-
ethylbenzothiazoline-6-sulphonic acid, hydro xyl and 1,1-diphenyl-2 picrylhydrazyl radicals. Effect
of extracts on ferrous ions chelating ability and total antioxidant
capacity were also investigated for
each extract. In addition, total
phenolic, flavonoid and flavonol content of rhubarb stalks ext racts were
determined. The stalk methanol e xtract showed significantly the highest
1,1-diphenyl-2- picrylhydrazyl radical scavenging activity, followed by stalks
ethylacetate extract, stalks hexane extract and
lastly, butylated hydroxytoluene. Ethylacetate extract showed the highest
2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) radical
scavenging activity with
IC50 value
of 59.7±1.1 μg/mL, fo llowed by
butylated hydroxytoluene and methanol e xt ract with IC50 value of
53.1±2.2 and 47.9±1.3 μg/ mL, respectively, whereas the highest hydroxyl
radical inhibition activity was found for methanol extracts with IC50
values of 75.1±2.9 μg/mL. The total antioxidant
activity was the highest in methanol and ethylacetate
extracts (284.1±11.6 and 237.6±10.0
μg ascorbic acid
equivalent for 1 mg extract, respectively). Methanol
and ethylacetate extracts at concentration
of 100 μg/ mL showed the highest chelating activity against ferrous ions
(96.3±2.2% and 89.8±1.5%, respectively). All rhubarb stalk extracts showed
pronounced antioxidant activities in a dose-dependent manner and the effects
depend strongly on the solvent used for extraction. The results showed that
extracts of the stalk of rhubarb exhibit antioxidant potential suggesting that
Rheum rhabarbarum L. is a promising plant.
Keywords:
Rheum rhabarbarum L., rhubarb, stalk, antioxidant activity,
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- ISSN: 2667-5072
- Başlangıç: 2018
- Yayıncı: Van Yüzüncü Yıl Üniversitesi
Sayıdaki Diğer Makaleler
KRONİK HASTALIK YÖNETİMİNDE TAMAMLAYICI VE ALTERNATİF TEDAVİ KULLANIMI VE HEMŞİRENİN ROLÜ
ANTIOXIDANT ACTIVITY of DIFFERENT EXTRACTS FROM STALK PART of RHUB ARB (RHEUM RHABARBARUM L.)
Mehmet BERKÖZ, Mehmet YILDIRIM, Mirosław KROŚNİAK, Renata FRANCİK, Oruç ALLAHVERDİYEV
Tahir KAHRAMAN, Mehmet BERKÖZ, Ramazan ÜSTÜN, M. Kürşat TÜRKDOĞAN
AŞİL TENDON YIRTIKLARI TEDAVİSİNDE AÇIK ve PERKÜTAN TEKNİKLERİN KARŞILAŞTIRILMASI