Olive oil Mill Wastewater triggers Hormonal and Phenolic metabolism shiftings in sunflower

The aim of the study was to investigate the effects of Olive oil Mill Wastewater (OOMW) in the sunflower in terms of hormonal and secondary metabolites (phenols). In all experiments as hormone, abscisic acid (ABA), gibberellic acid (GA), indolacetic acid (IAA), salicylic acid (SA) and jasmonic acid (JA), as phenol, Naringenin (NAR), Catechin (CATEC), Trans sinnapic acid (SINAP), Trans p-coumaric acid (PCOUMAR), Protocatechik acid (PROTOC), Trans cafeik acid (CAFFE), 2-5 Dihidro benzoic acid (DYHIDRO), Gallic acid (GALLIC), values were compared in control and experimental groups. OOMW was applied to the plants at various concentrations (1/1, 1/10, 1/100, 1/1000, 1/10000) for 3-days, 5- days and 10-days. Control plants are watered with water. Based on hormonal analysis; the most OOMW damage was in 5-day treatments, and in 10-day treatments it was partially healed. Similarly, 5-day changes in phenolic analyzes were found to be more severe. Consequently; it has been found that 1) SA is the most active hormone against OOMW stress, 2) In the 5-day trials, JA was active in conjunction with the SA, which was based on OOMW violence, similarly, hormones and phenolic substances are highly variable especially in the 5-day trial, 3), GA and IAA and ABA are generally quite lower levels in all experiments, 4) OOMW breaks hormonal balance in the plant, and 5) the secondary metabolite (phenol) metabolism has been changed considerably.

Anahtar Kelimeler:

Olive oil mill effluent, Olive oil waste waters, sunflower

PDF

___

[1] Banias G., C. Achillas, C. Vlachokostas, N. Moussiopoulos and M. Stefanou.. Environmental impacts in the life cycle of olive oil: a literature review. J Sci Food Agric; 97: 1686–1697 (2017).
[2] IOOC. International olive oil production costs study. Madrid: International Olive Oil Council (2011-2013).
[3] Dermeche, S., M. Nadour, C. Larroche, F. Moulti-Mati, P. Michaud. Olive mill wastes: Biochemical characterizations and valorization strategies. Process Biochem. 48 1532–1552 (2013).
[4] Brscic, K., Poljuha, D. and Krapac, M. Olive Residues - Renewable Source of Energy, Management of Technology - Step to Sustainable Production, Sibenic 10–12 June, Croatia, Embassy of Belgium in Croatia –Economic and Commercial Office (2009).
[5] Komnitsas, K. and Zaharaki, D. Pre-treatment of olive mill wastewaters at laboratory and mill scale and subsequent use in agriculture: legislative framework and proposed soil quality indicators. Resour Conserv Recy 69: 82–89 (2012).
[6] Andreozzi, R., Canterino, M., Di, Somma, I. Lo, Giudice, R., Marotta, R., Pinto, G., Pollio, A. Effect of combined physico-chemical processes on the phytotoxicity of olive mill wastewaters. Water Res 42: 1684–1692 (2008).
[7] El Hadrami, A., Belaqziz, M., El Hassni, M., Hanifi, S., Abbad, A., Capasso, R., Gianfreda, L., El Hadrami, I. Physico-chemical characterization and effects of olive oil mill wastewater fertirrigation on the growth of some mediterranean crops. J. Agron. 3 (4), 247–254, (2004).
[8] Gigliotti, G., Proietti, P., Said-Pullicino, D., Nasini, L., Pezzolla, D., Rosati, L., Porceddu, P.R. Co-composting of olive husks with high moisture contents: organic matter dynamics and compost quality. Int. Biodeterior. Biodegrad. 67, 8–14, (2012).
[9] El Hajjouji, H., Pinelli, E., Guiresse, M., Merlina, G., Revel, J.-C., Hafidi, M. Assessment of the genotoxicity of olive mill waste water (OMWW) with the Vicia faba micronucleus test. Mutat Res 634: 25–31 (2007).
[10] Aybeke M., Sıdal U., Olgun G., Kolankaya D. The Effect of Olive Oil Mill Effluent on the Mitotic Cell Division and Total Protein Amount of the Root Tips of Triticum aestivum L. (in Turkish). Tr.J.Of Biology 24: 127-140, (2000).
[11] Aybeke M.,Sıdal U., Hüseyin G. Structural changes in root tips of wheat (Triticum aestivum L) in response to Olive oil Mill waste water. Pak. J. Biol. Sci. 11 (15): 1957-1960, (2008).
[12] Aybeke, M. Genotoxic effects of olive oil wastewater on sunflower. Ecotoxicol. Environ. Saf. 147, 972–981. http://dx.doi.org/10.1016/j.ecoenv.2017.09.071 (2018).
[13] Sannac, S., Tadjiki, S., Moldenhauer, E. Single particle analysis using the Agilent 7700x ICP-MS. Agil. Technol (Publication number: 5991-2929EN). (2013).
[14] Box, J.D. Investigation of the Folin-Ciocalteau phenol reagent for the determination of polyphenolic substances in natural waters. Water Res. 17, 511–525, (1983).
[15] Li, H.B., Cheng, K.W., Wong, C.C., Fan, K.W., Chen, F., Jiang, Y. Evaluation of antioxidant capacity and total phenolic content of different fractions of selected microalgae. Food Chem. 102, 771–776, (2007).
[16] Hervé, D., Fabre, F., Berrios, E.F., Leroux, N., Al Chaarani, G., Planchon, C., Sarrafi, A., Gentzbittel, L. QTL analysis of photosynthesis and water status traits in sunflower (Helianthus annuus L.) under greenhouse conditions. J. Exp. Bot. 52 (362), 1857–1864, (2001).
[17] Müller, M., Munne´-Bosch, S. Rapid and sensitive hormonal profiling of complex plant samples by liquid chromatography coupled to electrospray ionization tandem mass spectrometry. Plant Method 7:37. (2011).
[18] Doganlar, Z.B. Physiological and genetic responses to pesticide mixture treatment of Veronica beccabunga. Water Air Soil Pollut. doi 10.1007/s11270-012-1350-y, (2012).
[19] Kazan, K. Diverse roles of jasmonates and ethylene in abiotic stress tolerance. Trends Plant Sci., 20, 4, 219-229, (2015).
[20] Tognetti, V.B., Van Aken, O., Morreel, K., Vandenbroucke, K., Van De, Cotte, B., De Clercq, I., Chiwocha, S., Fenske, R., Prinsen, E., Boerjan, W. Perturbation of indole-3-butyric acid homeostasis by the Redox, stress response and plant development UDP-glucosyltransferase UGT74E2 modulates Arabidopsis architecture and water stress tolerance. The Plant Cell 22, 2660–2679, (2010).
[21] Kammerhofer, N., Zoran, Radakovic, Jully, M. A. Regis, Petre, D., Radomira, Vankova, Florian, M.W. Grundler, Shahid, Siddique, Julia, Hofmann and Krzysztof, Wieczorek. Role of stress-related hormones in plant defence during early infection of the cyst nematode Heterodera schachtii in Arabidopsis. New Phytol. 207: 778–789, (2015).
[22] De Bruyne, L., Hofte, M,. De Vleesschauwer, D. Connecting growth and defense: the emerging roles of brassinosteroids and gibberellins in plant innate immunity. Molecular Plant 7: 943–959, (2014).
[23] Xia, Xiao-Jian, Yan-Hong, Zhou, Kai, Shi, Jie, Zhou, C.H., Foyer and Jing-Quan, Yu. Interplay between reactive oxygen species and hormones in the control of plant development and stress tolerance. J. Exp. Bot., 66, 10, 2839–2856, (2015). DOI: https://doi.org/10.1093/jxb/erv089.
[24] Kang, G., G. Li, T. Guo. Molecular mechanism of salicylic acid-induced abiotic stress tolerance in higher plants. Acta Physiol. Plant. 36: 2287–2297, (2014). doi:10.1007/s11738-014-1603-z.
[25] Aybeke, M. Fusarium infection causes genotoxic disorders and antioxidant-based damages in Orobanche spp. Microbiol. Res. 201, 46–51, (2017). http://dx.doi.org/10.1016/j.micres.2017.05.001
[26] Heil, M., Ibarra-Laclette, E., Adame-Alvarez, R.M.,Martinez, O., Ramirez-Chavez, E., Molina-Torres, J. et al. How plants sense wounds: damaged-self recognition is based on plant-derived elicitors and induces octadecanoid signaling. PLoS One; 7:e30537, (2012).
[27] Mazen, A. and Na-Sheng, Lin. Roles of plant hormones in the regulation of host–virus interactions. Mol. Plant Pathol. 16(5), 529–540, (2015).
[28] Venuprasad, M.P., Hemanth, Kumar, Kandikattu, Sakina, Razack, Farhath, Khanum. Phytochemical analysis of Ocimum gratissimum by LC-ESI–MS/MS and its antioxidant and anxiolytic effects. S. Afr. J. Bot. 92,151–158, (2014).
[29] Ahmed, I.M., Umme, Aktari, Nadira, Noreen, Bibi, Fangbin, Cao, Xiaoyan, H., Guoping Zhang, Feibo Wu. Secondary metabolism and antioxidants are involved in the tolerance to drought and salinity, separately and combined, in Tibetan wild barley. Environ. Exp. Bot. 111 1–12, (2015).
[30] Mehrotra, R., P. Bhalothia, P. Bansal, M.K. Basantani, V. Bharti, S. Mehrotra, Abscisic acid and abiotic stress tolerance – Different tiers of regulation. J. Plant Physiol.171 486–496, (2014).
[31] Jia, H.F., Chai, Y.M, Li, C.L., Lu, D., Luo, J.J., Qin, L. et al. (2011). Abscisic Acid Plays an Important Role in the Regulation of Strawberry Fruit Ripening. Plant Physiol. 157 (1):188–99. PMID: ISI:000294491800015. doi: 10.1104/pp.111.177311.
[32] Güler, Ç., Su Kalitesi. T.C. Sağlık Bakanlığı, Çevre sağlığı temel Kaynak dizisi, No: 43, (1997). 〈http://sbu.saglik.gov.tr/Ekutuphane/kitaplar/css43.pdf〉(Accessed on 15 March 2017).
[33] Martinez-Garcia, G., R.Th. Bachmann, C.J. Williams, A. Burgoyne, R.G.J. Edyvean. Int. Biodeter. Biodegr. 58, 231–238. (2006).
[34] Sharma, P., R. S. Dubey. Involvement of oxidative stress and role of antioxidative defense system in growing rice seedlings exposed to toxic concentrations of aluminum. Plant Cell Rep. 26: 2027–2038, (2007).
[35] Panda, S.K., Yamamoto, Y., Kondo, H., Matsumoto, H. Mitochondrial alterations related to programmed cell death in tobacco cells under aluminium stress. C. R. Biol. 331, 597–610, (2008).
[36] Malik, J.A., Goel, S., Kaur, N., Sharma, S., Singh, I., Nayyar, H. Selenium antagonises the toxic effects of arsenic on mungbean (Phaseolus aureus Roxb.) plants by restricting its uptake and enhancing the antioxidative and detoxification mechanisms. Environ. Exp. Bot. 77, 242–248, (2012).
[37] Kumar, M., Bijo, A.J., Baghel, R.S., Reddy, C.R.K., Jha, B. Selenium and Spermine alleviates cadmium induced toxicity in the red seaweed Gracilaria dura by regulating antioxidant system and DNA methylation. Plant Physiol. Biochem. 51, 129–138, (2012).
[38] Jasso-Chávez, R., Pacheco-Rosales, A., Lira-Silva, E., Gallardo-Pérez, J.C., García, N., Moreno-Sánchez, R., Toxic effects of Cr(VI) and Cr(III) on energy metabolism of heterotrophic Euglena gracilis. Aquat. Toxicol. 100, 329–338, (2010).
[39] El‐Jaoual, T., Cox, D.A. Manganese toxicity in plants. J. Plant Nutr. 21 (2), 353–386, (1998).
[40] Sierra, J., Martí, E., Garau, M.A., Crua˜nas, R. Effects of the agronomic use of olive oil mill wastewater field experiment. Sci. Total Environ. 378, 90–94, (2007).
[41] Kelepesi, S., Nikos, G.T. Olive mill wastes-a growing medium component for seedling and crop production of lettuce and chicory. Int. J. Veg. Sci. 15, 325–339, (2009).
[42] Celine, I.L.J., Pereira, R., Freitas, A.C., Rocha-Santos, T.A.P., Panteleitchouk, T.S.L., Duarte, A.C. Olive oil mill wastewaters before and after treatment: a critical review from the ecotoxicological point of view. Ecotoxicology 21, 615–629, (2012).
[43] Barbera A.C., C. Maucieri, V. Cavallaro, A. Ioppolo, G. Spagna. Effects of spreading olive mill wastewater on soil properties and crops, a review. Agric Water Manag.119 43– 53, (2013).