Fatih SEYİS, Emine YURTERİ, Aysel ÖZCAN, Haydar KÜPLEMEZ, Merve AKSU

Thiadiazuron ve Zeatin’in Helichrysum pallasii’de Bitki Rejenerasyonuna Etkisi

Helichrysum cinsi, tedavi edici etkileri olan ve halk hekimliğinde kullanılan birçok türü içermektedir. H. pallasii yörede geleneksel tıp ve süs amaçlı kullanılan bu türlerden bir tanesidir. Bu nedenle, bu türler için doku kültüründe alternatif mikroçoğaltım protokollerinin ve sekonder metabolit üretiminin optimize edilmesi gerektirmektedir. Bu çalışmada Zeatin (ZEA) ile kombine edilmiş Thidiazuran (TDZ)’ nın H. pallasi'de sürgün rejenerasyonu üzerine etkileri incelenmiştir. İn vitro koşullarda çimlendirilen bitkiciklerden alınan yaprak, gövde ve kök kısımları eksplant olarak kullanılmıştır. Sonuç olarak, en yüksek bitki rejenerasyonu kök eksplantlarına uygulanan 1 mg L-1 Thidiazuran (TDZ) + 0.1 mg L-1 Zeatin (ZEA) (%88.9) ve 1 mg L-1 TDZ (%85.7) uygulamalarından elde edilmiştir. En düşük bitki rejenerasyon yüzdesi (%16.7) ise yaprak eksplantları kullanılan kontrol uygulamasından elde edilmiştir.

Anahtar Kelimeler:

ölmez çiçek, mikro çoğaltım, doku kültürü, Thidiazuran, Zeatin

Effects of Thidiazuran and Zeatin on Plant Regeneration in Helichrysum pallasii

The genus of Helichrysum comprises many species which have therapeutical effects and used in folk medicine. H. pallasii is one of these species, used in the region for traditional medicine and ornamental purposes. Therefore, optimizing alternative micropropagation protocols of tissue culture conditions and secondary metabolite production for these species needs attention. The effect of Thidiazuran (TDZ) combined with Zeatin (ZEA) on shoot regeneration in H. pallasi was investigated during this study. The leaf, stem and root parts taken from the seedling germinated under in vitro conditions were used as explants. As a result, the root explants were more efficient compared to other explants in inducing plant regeneration using 1 mg L-1 Thidiazuran (TDZ) + 0.1 mg L-1 Zeatin (ZEA) (88.9%) and 1 mg L-1 TDZ treatment (85.7%). The lowest plant regeneration percentage (16.7%) was found in control medium using leaf explants.

Keywords:

immortal flower, micropropagation, tissue culture, Thidiazuran, Zeatin,

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Angioni, A., Barra, A., Arlorio, M., Coisson, J. D., Russo, M. T., Pirisi, F. M., Satta, M., & Cabras, P. (2003). Chemical composition, plant genetic differences, and Antifungal activity of the essential oil of Helichrysum italicum G. Don ssp. microphyllum (Willd) nym. Journal of Agricultural and Food Chemistry, 51(4), 1030-1034.
Appendino, G., Ottino, M., Marquez, N., Bianchi, F., Giana, A., Ballero, M., Sterner, O., Fiebich, B. L., & Munoz, E. (2007). Arzanol, an anti-inflammatory and Anti-HIV-1 Phloroglucinol α-pyrone from Helichrysum italicum ssp. microphyllum. Journal of Natural Products, 70(4), 608-612.
Baytop, T. (1986). Farmakognozi Ders Kitabı. Cilt I. İstanbul Üniversitesi Yayınları, No:3399, İstanbul.
Cragg, G. M., Body, M. R., Cardell, N., Grever, J., Schepartz, M., & Spader, K. (1993). The Role of Plants in the Drug Discovery Program of the US Manupilation. International Crop Science 1 Maddison, USA: Crop Science Society of America.
Davis, P. H. (1975). Flora of Turkey and the East Aegean Islands. Edinburgh, UK: Edinburgh University Press.
Davis, P. H., Mill, R. R., & Tan, K. (1988). Flora of Turkey and the East Aegean Islands. Edinburgh, UK: Edinburgh University Press.
Đorđević, A., Lazarević, J., Šmelcerović, A., & Stojanović, G. (2013). The case of Hypericum rochelii Griseb. and Schenk and Hypericum umbellatum A. Kern. essential oils: Chemical composition and antimicrobial activity. Journal of Pharmaceutical and Biomedical Analysis, 77, 145-148.
Eröztürk, N. (2000). Bir Yudum Sağlık. Anahtar Kitap Yayınları, İstanbul.
Farnsworth, N. R. (1993). Ethnopharmacology and future drug development: The North American experience. Journal of Ethnopharmacology, 38(2-3), 137-143.
Ghasemi Pirbalouti, A., Hashemi, M., & Ghahfarokhi, F. T. (2013). Essential oil and chemical compositions of wild and cultivated Thymus daenensis Celak and Thymus vulgaris L. Industrial Crops and Products, 48, 43-48.
Giovannini, A., Amoretti, M., Savona, M., Di Guardo, A., & Ruffoni, B. (2003). Tissue Culture in Helichrysum spp. Acta Horticulturae, 616, 339-342.
Guner, A., Ozhatay, N., Ekim, T., & Baser, K. H. C. (2000). Flora of Turkey and the East Aegean Islands. Edinburgh, UK: Edinburgh University Press.
Kalogeropoulos, N., Kaliora, A. C., Artemiou, A., & Giogios, I. (2014). Composition, volatile profiles and functional properties of virgin olive oils produced by two-phase vs three-phase centrifugal decanters. Food Science and Technology, 58(1), 272-279.
Lawrence, B. M. (1998). Helichrysum oil and extract. Perfumer Flavorist, 23(5), 55–59.
Li, Y., Kong, D., & Wu, H. (2013). Analysis and evaluation of essential oil components of Cinnamon barks using GC–MS and FTIR spectroscopy. Industrial Crops and Products, 41, 269-278
Machado, D. G., Cunha, M. P., Neis, V. B., Balen, G. O., Colla, A., Bettio, L. E., Oliveira, Á., Pazini, F. L., Dalmarco, J. B., Simionatto, E. L., Pizzolatti, M. G., & Rodrigues, A. L. (2013). Antidepressant-like effects of fractions, essential oil, carnosol and betulinic acid isolated from Rosmarinus officinalis L. Food Chemistry, 136(2), 999-1005.
Mebatsion, H., Paliwal, J., & Jayas, D. (2012). Evaluation of variations in the shape of grain types using principal components analysis of the elliptic fourier descriptors. Computers and Electronics in Agriculture, 80, 63-70.
Murashige, T., & Skoog,, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, 15(3), 473-497.
Nigg, H. N., & Seigler, D. (1992). Phytochemical Resources for Medicine and Agriculture. Springer, USA.
Olmedo, R., Nepote, V., & Grosso, N. R. (2014). Antioxidant activity of fractions from oregano essential oils obtained by molecular distillation. Food Chemistry, 156, 212-219.
Perrini, R., Ruta, C., & Morone Fortunato, I. (2009). Regeneration through organogenesis from leaves of Helichrysum italicum (Roth) G. Don. Acta Horticulturae, 812, 217-222.
Quassinti, L., Bramucci, M., Lupidi, G., Barboni, L., Ricciutelli, M., Sagratini, G., Papa, F., Caprioli, G., Petrelli, D., Vitali, L. A., Vittori, S., & Maggi, F. (2013). In vitro biological activity of essential oils and isolated furanosesquiterpenes from the neglected vegetable Smyrnium olusatrum L. (Apiaceae). Food Chemistry, 138(2-3), 808-813.
Rossi, D., Guerrini, A., Paganetto, G., Bernacchia, G., Conforti, F., Statti, G., Maietti, S., Poppi, I., Tacchini, M., & Sacchetti, G. (2013). Croton lechleri Müll. Arg. (Euphorbiaceae) stem bark essential oil as possible mutagen-protective food ingredient against heterocyclic amines from cooked food. Food Chemistry, 139(1-4), 439–447.
Soković, M., Tzakou, O., Pitarokili, D., & Couladis, M. (2002). Antifungal activities of selected aromatic plants growing wild in Greece. Die Nahrung, 46(5), 317–320.
Sumbul, H., Gokturk, R. S., & Dusen, O. D. (2003). A new endemic species of Helichrysum Gaertn. (Asteraceae–Inuleae) from south Anatolia. Botanical Journal of the Linnean Society, 141, 251–254.