ÖZGE ŞAHİN, MEHMET BURAK TAŞKIN, Emre Can KAYA, Havva TASKIN

Tavuk Gübresi Biyokömürünün Çeltik Bitkisi Arsenik Alımı ve Arsenik Düzeyleri Üzerine Etkisi ve Oksidatif Stres İle İlişkisi

Bu çalışmada, çeltik bitkisinde arsenik (As) birikiminin azaltılması üzerine biyokömürün etkisi araştırılmıştır. Uygulamalar sırasıyla; kontrol, 60 mg kg-1 As (As NaAsO2'den uygulanmıştır.) ve 60 mg kg-1 As + 20 g kg-1 biyokömür şeklindedir. Biyokömür uygulamasına bağlı olarak, As ile kirlenmiş toprakta yetiştirilen çeltik bitkisinin kuru ağırlığı artış göstermiştir. 60 mg kg-1 As uygulaması ile çeltik bitkisinin kuru ağırlığı kontrole göre azalmıştır. As uygulaması ile çeltik bitkisinin As konsantrasyonu artarken, 20 g kg-1 biyokömür uygulaması ile bitkinin As konsantrasyonunu önemli düzeyde azaltmıştır. Arsenik toksisitesi ile bitkide H2O2 birikimi artmış, ilave edilen biyokömür uygulamasına bağlı olarak H2O2 konsantrasyonu azalmıştır. Katalaz (CAT) ve askorbat peroksidaz (APX) aktiviteleri biyokömür uygulaması ile önemli düzeyde artmıştır. Arsenik uygulamasına bağlı olarak çeltik bitkisinin N, S ve Zn konsantrasyonlarını artarken, Cl, Fe, Cu ve Mn konsantrasyonlarını azalmıştır. Kontrol ve As uygulamalarıyla karşılaştırıldığında, biyokömür uygulamasının bitki N, P, K ve S konsantrasyonlarını önemli düzeyde arttırdığı belirlenmiştir. Çeltik bitkisinde As birikimini/toksisitesini önlemek için tavuk gübresinden elde edilen biyokömürün kullanılabileceği sonucuna varılmıştır

Poultry Manure Biochar Reduces Arsenic Induced Oxidative Stress and Arsenic Levels in Rice Plants

The effectiveness of biochar on mitigating excessive arsenic (As) accumulation by rice plants was investigated. The treatments were as follows: control, 60 mg kg-1 As (As applied from NaAsO2) and 60 mg kg-1 As + 20 g kg-1 biochar. Biochar application to As contaminated soil enhanced the dry weight of rice plants. Dry weight of rice plants decreased by 60 mg kg-1 As treatment compared to control. Application of As increased As concentration of rice plants while 20 g kg-1 poultry manure biochar supply to the As contaminated soil significantly decreased the As concentration. Arsenic toxicity induced H2O2 accumulation however the application of biochar reduced H2O2 concentration of plants. Catalase (CAT) and ascorbate peroxidase (APX) activities significantly increased by biochar. Arsenic significantly increased N, S and Zn while decreased Cl, Fe, Cu and Mn concentration of plants. Biochar treatments significantly increased N, P, K and S concentrations of the plants as compared to control and As treated plants. It can be concluded that poultry manure biochar can be used for the prevention of As accumulation in rice plants

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