Nosema ceranae Enfeksiyonunun İran, Mazandaran İlinde Mikroskopik ve Moleküler Çalışması

Nosema ceranae, tüm kıtalarda Apis mellifera'dan mantar paraziti olarak rapor edilmiştir. Bal arısı kolonilerinde yaşam süresinin azalmasına, nüfus azalmasına, üretimin azalmasına ve koloni kayıplarına neden olur. Bu çalışmada Mazandaran ili arılıklarında N.ceranae yaygınlığının belirlenmesi amaçlanmıştır. Bu çalışmada, otuz iki arılıktan rastgele 320 kovan seçtik ve arılıklardan yirmi yaşlı bal arısı topladık. Toplanan örnekler mikroskop ve moleküler yöntemlerle incelendi. Mikroskobik incelemelerde arılıkların %78,12'sinin Nosema spp belirlendi. Ayrıca N. ceranae, PCR ile %84.37'sinde tespit edilirken, hiçbir numune N. apis ile enfekte olmamıştır. Sıralı örneklerin gen hizalama analizi sonucunda arılıklarda N. ceranae enfeksiyonunun varlığı doğrulandı. Elde edilen sonuçlara göre Mazandaran ilindeki arılıklarda yüksek oranda N.ceranae tespit edilmiştir.

Anahtar Kelimeler:

Nosema ceranae, Bal arısı, PCR, İran

A MICROSCOPY AND MOLECULAR STUDIES OF NOSEMA CERANAE INFECTION IN MAZANDARAN PROVINCE OF IRAN

Nosema ceranae as a fungal parasite has been reported from the Apis mellifera in all continents. It causes reduced longevity, depopulation, decreased production, and colony losses in honeybee colonies. This study aimed to determine the prevalence of N.ceranae in the apiaries of Mazandaran province. In this study, we randomly selected 320 hives from thirty-two apiaries and collected twenty old honeybees from the apiaries. The collected samples were examined by microscopy and molecular methods. The results of the microscopic examinations showed that 78.12% of apiaries were infected with Nosema spp. In addition, N. ceranae was identified 84.37% of apiaries by PCR, while no samples were infected by N. apis. Blast analysis of the sequenced samples confirmed the presence of N. ceranae infection in the apiaries. Based on the obtained results, a high frequency of N.ceranae was detected in apiaries in Mazandaran province.

Keywords:

Nosema ceranae, Honeybee, PCR, Iran,

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