Leyla PAŞAYEVA, Ufuk İNCE, Havva KARADELİ, Funda MARAŞLI, Dilbeste ÖZTÜRK, Selin URMAK, Osman TUGAY

Stenocephalae Bunge. Seksiyonuna Dâhil Olan Türlerin Antidiyabetik, Antimikrobiyal Etkisi ve Fitokimyasal İçeriğinin İncelenmesi

Bu çalışmanın amacı, Stenocephalae seksiyonuna dâhil olan Cousinia davisiana, C. foliosa, C. ramosissima ve C. stenocephala türlerinin hipoglisemik ve antimikrobiyal etkisinin incelenmesidir. Çalışma kapsamında bitkilerin toprak üstü kısmı toplandıktan sonra metanol ile oda sıcaklığında maserasyon yöntemiyle ekstreleri hazırlanmış ve bu ekstrelerin α-amilaz, α-glikozidaz inhibitör ve antimikrobiyal etkisi tayin edilmiştir. Elde edilen sonuçlar değerlendirildiğinde bitkiler arasında C. ramosissima ekstresinin 246.784±1.528 µg mL-1 IC50 değeri ile diğer ekstrelere ve akarboza göre daha yüksek α-amilaz etki ve α-glikozidaz (155.458±0.279 µg mL-1) etki, ayrıca çalışılan ekstrelerin orta derecede antimikrobiyal etki gösterdiği bulunmuştur. Bunun dışında aktif bulunan ekstrenin LC-MS/MS ile yapılan inceleme sonucunda fenolik bileşikler ve flavonoitleri yoğun olarak içerdiği belirlenmiştir. Bu çalışmanın sonuçları ile özellikle C. ramosissima ekstresinin etkili α-amilaz ve α-glikozidaz inhibitör etkiye sahip olduğu ilk kez kanıtlanmakla birlikte, bu ekstrelerin postprandiyal glikoz düzeylerini azaltmaya yardımcı olabileceğini göstermek için daha ileri çalışmalara ihtiyaç duyulmaktadır.

Anahtar Kelimeler:

Cousinia, Asteraceae, hipoglisemik etki, fenolik bileşikler, flavonoitler

Investigation of Antidiabetic, Antimicrobial Effects and Phytochemical Composition of The Species From Stenocephalae Bunge. Section

The aim of this study is to examine the hypoglycemic and antimicrobial effects of Cousinia davisiana, C. foliosa, C. ramosissima, and C. stenocephala species, which are included in the Stenocephalae section. Within the scope of the study, after the above-ground parts of the plants were collected, their extracts were prepared by maceration at room temperature with methanol, and the α-amylase, α-glucosidase inhibitory, and antimicrobial effects of these extracts were determined. When the results were evaluated, among the plants, the C. ramosissima extract was found to be more active in α-amylase with 246.784±1.528 µg mL-1 values compared to other extracts and higher α-amylase activity and α-glucosidase (155.458±0.279 µg mL-1) effect compared to acarbose. It was also found that the extracts showed moderate antimicrobial activity. Apart from this, it was determined that the active extract contained phenolic compounds and flavonoids intensely as a result of the examination by LC-MS/MS. Although the results of this study prove for the first time that especially C. ramosissima extract has effective α-amylase and α-glucosidase inhibitory effects, further studies are needed to show that these extracts can help reduce postprandial glucose levels.

Keywords:

Cousinia, Asteraceae, hypoglycemic effect, phenolic compounds, flavonoids,

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